✓ Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is an cell—specific mitogen that structurally related to platelet-derived (PDGF). induces angiogenesis in vivo and may play a critical role tumor angiogenesis. Using immunohistochemical analysis, the authors demonstrated presence of VEGF/VPF protein surgical specimens glioblastoma multiforme cultured glioma cells. By means enzyme-linked immunosorbent assay (ELISA) cell supernatants, showed variably secreted by all...

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), an cell-specific cytokine, induces proliferation of cells and increases vascular dramatically. All gliomas secrete significant amounts VEGF, whereas meningiomas are variable in expression. Thus, we sought to determine whether the extent VPF/VEGF expression correlated with differences brain edema associated these tumors.Meningioma tissue samples from 37 patients (15 men, average age 65 +/- 13 yr; 22 women, 60 10 yr)...

Currently, the effectiveness of minimally invasive evacuation intracerebral hemorrhage (ICH) utilizing endoscopic method is uncertain and technique considered investigational. The authors analyzed their experience with this in terms case selection, surgical technique, long-term results.The performed a retrospective analysis clinical radiographic data obtained 68 patients treated endoscope-assisted ICH evacuation. Rebleeding, morbidity, mortality were recorded as primary end points. Hematoma...

Background. Carriage and subsequent infection with methicillin resistant S. aureus (MRSA) its transmission between hospital community settings have not been studied in dialysis patients their contacts. Methods. Surveillance for nasal MRSA carriage among patients, healthcare workers (HCWs) family members a centre was prospectively undertaken during three time periods within 1 year. Molecular typing used to determine epidemiological relationship. Results. Among 1687 samples collected,...

Three hundred palmar hyperhidrosis (PH) patients have been treated with video endoscopic laser sympathectomy during the last 2 years. Monitoring skin perfusion (PSP) and temperature (PST) has used intraoperatively to aid confirmation of correct sympathetic segment for ablation. The preoperative postoperative PSP PST response (SSR) also measured evaluate therapeutic effect this method. An apparent increase would occur after interruption T2 segment, then a gradual elevation follow extirpation...

A total of 71 fusidic acid-resistant Staphylococcus aureus (45 methicillin-resistant and 26 methicillin-susceptible) isolates were examined for the presence resistance determinants. Among 45 S. (MRSA), isolates, 38 (84%) had fusA mutations conferring high-level to acid (the MIC was ≥128 μg/ml 22/38), none fusB, 7 (16%) fusC. For methicillin-susceptible (MSSA), only 3 possessed mutations, but 15 (58%) fusB 8 (31%) Low-level (MICs ≤ 32 μg/ml) found in most fusB- or fusC-positive isolates. 41...

Abstract Background and Objectives In Taiwan, more than two million people have the betel quid (BQ) chewing habit which is a risk factor related to premalignant lesion squamous cell carcinoma of oral cavity. We developed light‐emitting diode (LED) array combined with topical 5‐aminolevulinic acid (ALA) for photodynamic therapy (PDT) evaluated its effectiveness treatment lesions. Study Design/Materials Methods compared ALA‐PDT effect homemade LED that commercial light source on cultured...

ABSTRACT Susceptibilities to 16 antimicrobial agents were determined by measurement of MICs for 344 isolates anaerobic bacteria recovered from patients with significant infections. Resistance rates varied among and the species tested. The β-lactams more active in gram-positive than gram-negative anaerobes. meropenem was low (<1%). For β-lactam-β-lactamase inhibitors, piperacillin-tazobactam most all (resistance, <6%). resistance cefoxitin (31 65%) clindamycin (50 70%) non- Bacteroides...

Abstract Background and Objectives Multidrug‐resistant (MDR) Pseudomonas aeruginosa infection is becoming a critical problem worldwide. Currently, only limited therapeutic options are available for the treatment of infections caused by MDR P. , therefore, development new alternative treatments needed. Toluidine blue O (TBO) an effective antibacterial photosensitizing agent against various bacteria. However, reports on photosensitization bacteria limited. This study aims to determine in vitro...

The full-length sequences of the groESL genes (also known as cpn10/60) Streptococcus anginosus, constellatus, gordonii, and sanguis near sequence intermedius, bovis, mitis, mutans, oralis, salivarius were determined. lengths groES from 10 species listed above ranged 282 to 288 bp, groEL determined for 4 (S. S. sanguis) revealed that each was 1,623 bp. intergenic region (spacer) between varies in size (15 111 bp) species. variation among tested greater (62.1 95.1% nucleotide identities) than...

Abstract Background and Objectives Photodynamic therapy (PDT) has been proposed as an alternative approach in overcoming multidrug resistance (MDR) phenotype. To verify whether 5‐aminolevulinic acid (ALA)‐mediated PDT is effective MDR cells, we studied the protoporphyrin IX (PpIX) content, intracellular localization, phototoxicity human breast cancer cells MCF‐7 derived subline, MCF‐7/ADR. Study Design/Materials Methods The fluorescence kinetics of ALA‐induced PpIX was evaluated by...

ObjectivesSmall colony variants (SCVs) of Staphylococcus aureus are associated with persistent and drug-resistant infections. We demonstrated for the first time emergence SCVs in a patient vancomycin-intermediate S. (VISA) infection during long-term treatment daptomycin.

We analyzed the occurrence and mechanisms of fusidic acid resistance present in staphylococci isolated from 59 healthy volunteers. The fingers volunteers were screened for presence staphylococci, collected isolates tested to acid. A total 34 resistant staphylococcal strains (all coagulase-negative) 22 individuals (22/59, 37.3%). Examination genes revealed that acquired fusB or fusC was Staphylococcus epidermidis, capitis subsp. urealyticus, hominis hominis, warneri haemolyticus. Resistance...

To evaluate the feasibility of detecting bacterial pathogens directly from clinical brain abscess specimens by polymerase chain reaction (PCR) amplification and sequencing 16S ribosomal deoxyribonucleic acid (rDNA).A total 14 were tested both culture PCR amplification, targeting full-length or a partial region rDNA. rDNA is known to be conserved in bacteria. Sequencing partial-length was performed. The sequence data compared with sequences National Center for Biotechnology Information...

Recent studies have revealed a correlation between specific genetic changes, such as loss of chromosome 1p and 19q, sensitivity oligodendroglial neoplasm to radiotherapy chemotherapy; epigenetic changes also play an important role in some tumors. In this retrospective study, we analyzed chromosomal alterations 17 loci promoter methylation status 8 tumor-related genes 49 tumors (29 WHO grade II 11 III oligodendrogliomas; 7 2 oligoastrocytomas) using quantitative microsatellite analysis...

Anesthesia can become inadequate inadvertently or by misjudgment during surgery emergence, and the surgical stress pain stimulation will increase without adequate treatment. Overt may activate sympathetic nervous system, blood level of catecholamines, lead to splanchnic arterial vasoconstriction.We divided 30 male Wistar rats into following 3 groups: control, (SSP), + dexmedetomidine (SSP Dex). The received midline laparotomy exteriorize a segment terminal ileum for microcirculation...

The supraorbital keyhole approach has been used in anterior skull base tumor and aneurysm surgery. However, there are debates regarding the safety limitations of this kind approach.To determine feasibility potential benefits surgical robotic technology minimally invasive neurosurgery.Two fresh cadaver heads were studied with da Vinci Surgical System 0° 30° stereoscopic endoscopes to visualize neuroanatomy. ability system suture place clips under was tested.The throughout transeyebrow...