A5016159315- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Enzyme Production and Characterization
- Advanced Glycation End Products research
- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Atherosclerosis and Cardiovascular Diseases
- Adenosine and Purinergic Signaling
- Peroxisome Proliferator-Activated Receptors
- Quantum, superfluid, helium dynamics
- Protein Kinase Regulation and GTPase Signaling
- Adipose Tissue and Metabolism
- Atomic and Subatomic Physics Research
- Cardiovascular Health and Disease Prevention
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Microbial Metabolites in Food Biotechnology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiovascular Function and Risk Factors
- Food composition and properties
- Neuropeptides and Animal Physiology
- Biofuel production and bioconversion
- High voltage insulation and dielectric phenomena
Hoshi University
2016-2025
Ebara (Japan)
2010-2025
Chiba University
2020
Saga University
2013-2015
Obayashi (Japan)
2013
Toshiba (Japan)
1987-2012
Tohoku University
2005
RIKEN
1995-2005
The University of Tokyo
1950-2003
Toho University
2003
The phosphatidylinositol 3-kinase (PI3-K) pathway, which activates serine/threonine protein kinase Akt, enhances endothelial nitric oxide synthase (eNOS) phosphorylation and (NO) production. We investigated the involvement of PI3-K/Akt pathway in relaxation responses to acetylcholine (ACh) clonidine a new type 2 diabetic model (streptozotocin plus nicotinamide-induced mice). Plasma glucose insulin levels were significantly elevated our model, intravenous tolerance tests revealed clear...
We previously reported that in mesenteric arteries from aged Otsuka Long-Evans Tokushima fatty (OLETF) rats (a type 2 diabetes model) endothelium-derived hyperpolarizing factor (EDHF)-type relaxation is impaired while contracting (EDCF)-mediated contraction enhanced (Matsumoto T, Kakami M, Noguchi E, Kobayashi Kamata K. Am J Physiol Heart Circ 293: H1480-H1490, 2007). Here we investigated whether acute and/or chronic treatment with metformin might improve this imbalance between the effects...
In isolated superior mesenteric artery rings from age-matched control rats and streptozotocin (STZ)-induced diabetic rats, we investigated the role of cAMP in endothelium-derived hyperpolarizing factor (EDHF)-type relaxation. The ACh-induced EDHF-type relaxation was significantly weaker STZ-induced than both groups it attenuated by 18α-glycyrrhetinic acid (18α-GA), an inhibitor gap junctions, enhanced IBMX, a cAMP-phosphodiesterase (PDE) inhibitor. These responses were very similar magnitude...
Insulin resistance is associated with atherosclerosis, but its mechanism unknown. It has been reported that insulin receptor substrate (IRS)-1 deficient (IRS-1-/-) mice showed without type 2 diabetes, whereas the IRS-2 (IRS-2-/-) diabetes.We investigated neointima formation in IRS-1-/- and IRS-2-/- at 8 20 weeks. The much greater than wild-type At weeks, had mice, which more enhanced mice. dyslipidemia, hypertension, resistance. metabolic abnormalities expression was detected, IRS-1 not...
Experiments were designed to investigate the involvement of superoxide anions in attenuated endothelium‐dependent relaxation rat aorta from streptozotocin (STZ)‐induced diabetic rats. The responses acetylcholine (ACh, 10 −7 m ) helical strips precontracted with noradrenaline (NA, 5 × −8 ∼3 significantly decreased STZ‐induced recovery phase after single administration ACh rats was more rapid than those control vessels. Preincubation aortic dismutase (SOD, 60 u ml −1 normalized ACh, whereas...
We investigated whether the balance between endothelium-derived relaxing factors (EDRFs) and contracting (EDCFs) might be altered in mesenteric arteries from aged Otsuka Long-Evans Tokushima Fatty (OLETF) rats (a Type 2 diabetic model) [vs. age-matched control (LETO) rats]. ACh-induced relaxation was impaired OLETF group, a tendency for to reverse at high ACh concentrations observed both groups. This abolished by indomethacin. Nitric oxide- and/or hypolarizing factor-mediated protein...
Accumulating evidence demonstrates that dietary intake of <i>n</i>-3 polyunsaturated fatty acids (PUFAs) is associated with a reduced incidence several cardiovascular diseases involve endothelial dysfunction. However, the molecular mechanism remains unclear. We previously reported mesenteric arteries from type 2 diabetic Otsuka Long-Evans Tokushima (OLETF) rats exhibit dysfunction, leading to an imbalance between endothelium-derived vasodilators [namely, nitric oxide (NO) and hyperpolarizing...
In type 2 diabetes, impaired insulin-induced Akt/endothelial nitric oxide synthase (eNOS) signaling may decrease the vascular relaxation response. Previously, we reported that this response was negatively regulated by G protein-coupled receptor kinase (GRK2). study, investigated whether/how in aortas from ob/ob mice (a model of diabetes) GRK2 and β-arrestin might regulate signaling. Endothelium-dependent measured aortic strips. GRK2, 2, Akt/eNOS pathway proteins activities were mainly...
Diabetic vascular complications are associated with endothelial dysfunction. Various plant-derived polyphenols benefit cardiovascular function by protecting nitric oxide (NO) production through as yet unclear mechanisms. This study compared the effects of two structurally similar polyphenols, Morin (MO) and Quercetin (QU), on in isolated aorta from control streptozotocin (STZ)-induced diabetic mice. Vascular under treatment MO, QU, various signaling pathway modulators was measured isometric...
We investigated the effects of chronic pravastatin treatment on impaired endothelium‐dependent relaxation seen in aortae from established streptozotocin (STZ)‐induced diabetic rats. Starting at 6 weeks diabetes, (10 mg kg −1 ) was administered to STZ‐induced rats for 4 weeks. The increased total cholesterol and low‐density lipoprotein (LDL) levels were not restored normal by pravastatin. Aortae pravastatin‐treated did show an acetylcholine. expression mRNA endothelial nitric oxide synthase...
Endothelin (ET)-1 is a likely candidate for key role in diabetic vascular complications. However, no abnormalities the responsiveness to ET-1 have been identified chronic stage of type 2 diabetes. Our goal was look roles played by ET receptors (ET A and B ) mesenteric artery Goto-Kakizaki (GK) rat identify molecular mechanisms involved. Using arteries from later-stage (32–38 wk old) individuals, we compared ET-1-induced contraction relaxation induced selective receptor agonist IRL1620...
Arteries from hypertensive subjects are reportedly hyperresponsive to 5-hydroxytryptamine (5-HT), but it remains unclear whether this is true in chronic type 2 diabetes. We have assessed responses 5-HT shown by mesenteric arteries diabetic ob/ob mice (27-32 weeks old) and identified the molecular mechanisms involved.Contractions of rings were examined vitro. Activation RhoA, Rho kinase Src was measured Western blotting or modified enzyme-linked immunosorbent assay.Concentration-dependent...
In diabetic states, hyperinsulinemia may negatively regulate Akt/endothelial nitric oxide synthase (eNOS) activation. Our main aim was to investigate whether and how insulin might Akt/eNOS activities via G protein-coupled receptor kinase 2 (GRK2) in aortas from ob/ob mice. Endothelium-dependent relaxation measured aortic rings mice (a type diabetes model). GRK2, β-arrestin2, signaling-pathway protein levels were mainly assayed by Western blotting. Plasma significantly elevated...
Scope Endothelial dysfunction is a critical factor during the initiation of diabetic cardiovascular complications. Polyphenols may represent beneficial dietary components eliciting protection. Although we previously reported that polyphenol morin (MO) ameliorated diabetes‐induced endothelial dysfunction, underlying mechanism remains unclear. Here, investigated protective effects and mechanisms MO in streptozotocin STZ induced aorta dysfunction. Methods results Diabetes was by tail vein...
Mineralocorticoid receptor (MR) blockers reduce cardiovascular complications as MRs play a crucial role in regulation. Diabetic are caused by vascular endothelial dysfunction. This study used type 2 diabetic mouse model (DM) to investigate whether esaxerenone (ESAX), an MR blocker, ameliorates ESAX (3 mg/kg/d) was administered via diet KK-Ay mice or C57BL/6J mice, nondiabetic control (Control), for 8 weeks, and metabolic parameters blood pressure were measured. Vascular responses of the...