A5084503235- Nuclear physics research studies
- Atomic and Molecular Physics
- Advanced Chemical Physics Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Chronic Myeloid Leukemia Treatments
- Astronomical and nuclear sciences
- Acute Myeloid Leukemia Research
- Quantum Chromodynamics and Particle Interactions
- High-Energy Particle Collisions Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Chronic Lymphocytic Leukemia Research
- Congenital Diaphragmatic Hernia Studies
- Virus-based gene therapy research
- Metabolism, Diabetes, and Cancer
- Cardiovascular Disease and Adiposity
- Nuclear Physics and Applications
- Immune Cell Function and Interaction
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
- Extracellular vesicles in disease
- IL-33, ST2, and ILC Pathways
- Genetics and Neurodevelopmental Disorders
- Energy and Environment Impacts
Tokyo Medical University
2016-2024
The University of Tokyo
1999-2014
Systems Biology Institute
2010
Japan Science and Technology Agency
2006
Tohoku University
1999-2003
Japan Radioisotope Association
2002
RIKEN
2001
Adipose tissue expression and circulating concentrations of monocyte chemoattractant protein-1 (MCP-1) correlate positively with adiposity. To ascertain the roles MCP-1 overexpression in adipose, we generated transgenic mice by utilizing adipocyte P2 (aP2) promoter (aP2-MCP-1 mice). These had higher plasma increased macrophage accumulation adipose tissues, as confirmed immunochemical, flow cytometric, gene analyses. Tumor necrosis factor-α interleukin-6 mRNA levels white non-esterified fatty...
Ecotropic viral integration site 1 (Evi1), a transcription factor of the SET/PR domain protein family, is essential for maintenance hematopoietic stem cells (HSCs) in mice and overexpressed several myeloid malignancies. Here, we generate reporter which an internal ribosome entry (IRES)-GFP cassette knocked-in to Evi1 locus. Using these mice, find that predominantly expressed long-term HSCs (LT-HSCs) adult bone marrow, stem/progenitor fraction aorta-gonad-mesonephros, placenta, fetal liver...
Hepatic insulin signalling involves receptor substrates (Irs) 1/2, and is normally associated with the inhibition of gluconeogenesis activation lipogenesis. In diabetes obesity, no longer suppresses hepatic gluconeogenesis, while continuing to activate lipogenesis, a state referred as 'selective resistance'. Here, we show that resistance' caused by differential expression Irs1 Irs2 in different zones liver. We demonstrate Irs2-knockout mice develop resistance', whereas lacking Irs1, or both...
Relapse of chronic myeloid leukemia (CML) is triggered by stem cells with a reconstituting capacity similar to that hematopoietic (HSCs) and CML are source resistance in drug therapy tyrosine kinase inhibitors (TKIs). Ecotropic viral integration site 1 (EVI1), key transcription factor HSC regulation, known predict poor outcomes malignancies, however, incapability prospective isolation EVI1-high leukemic precludes the functional evaluation intraindividual cells. Introduction into...
The reaction rate of the stellar 13C(alpha,n)16O, which is currently considered to be main neutron source for slow (s) process at low energies, has been rederived using direct alpha-transfer 13C(6Li,d)17O leading subthreshold state 6.356 MeV in 17O. contribution found much smaller than accepted predictions s process, indicating less a role this as s-process scenario low-mass stars asymptotic giant branch.
Levels in ${}^{22}\mathrm{Mg}$ between 4 and 7 MeV excitation energy have been populated a high-resolution study of the ${}^{24}\mathrm{Mg}{(p,t)}^{22}\mathrm{Mg}$ reaction. Two new states observed at energies ${E}_{x}=5090$ 6323 keV, while two were 5962 6046 keV. The precision measured for several other levels has improved substantially. In addition, state 8141 keV was ${}^{23}\mathrm{Mg}.$ Using spin parity restrictions from present previous work, we discuss $T=1$ analog assignments $A=22$...
Mesenchymal stem cells (MSCs) are promising therapeutic tools in regenerative medicine. In particularly adipose tissue derived MSC (AMSC) has powerful potential for the therapeutics of rheumatoid arthritis (RA) because these can control immune balance. RA systemically occurs autoimmune disease. Interestingly, IL-1 receptor antagonist deficient (IL-1ra-/-) mice induce inflammation joints like RA. therapy, although AMSC improves activity, it is little known to play roles extracellular...
The ${}^{20}\mathrm{Ne}(p,t){}^{18}\mathrm{Ne}$ reaction has been studied in order to investigate the properties of ${}^{18}\mathrm{Ne}$ excited states. A missing ${3}^{+}$ state ${}^{18}\mathrm{Ne},$ which could have a great influence on ${}^{17}\mathrm{F}(p,\ensuremath{\gamma}){}^{18}\mathrm{Ne}$ rate, was searched for, but existence this not clearly verified. spins and parities 5.11- 5.15-MeV states were assigned tentatively by angular distribution measurements, as ${2}^{+}$...
DICER1 syndrome is a tumor predisposition caused by familial genetic mutations in DICER1. Pathogenic variants of have been discovered many rare cancers, including cystic liver tumors. However, the molecular mechanisms underlying lesions induced these remain unclear. In present study, we sought to gain better understanding pathogenesis generating mouse model liver-specific syndrome. The developed bile duct hyperplasia with fibrosis, similar congenital hepatic as well tumors resembling those...
The innate cytokine response to nucleic acid is the most challenging problem confronting practical use of medicine. degree stimulation strongly depends on length acid. In this study, we developed a 30-nucleotide single-strand RNA, termed "guide hairpin RNA (ghRNA, ghR)", that has physiological function similar miRNA and siRNA. ghR caused no either in vitro or vivo. addition, its structure does not contain passenger strand seed sequence, reducing unwanted gene repression relative existing...
Humanized mice, which refers to immunodeficient mice repopulated with the human immune system, are powerful tools for study in field of immunology. It has been difficult, however, generate these transgenic (Tg) directly from such strains as NOD/SCID mouse. In this study, we describe a method developed by us generation Tg on an background. First, obtained fertilized eggs efficiently means vitro fertilization (IVF); then, attempted CAG-EGFP background, finding that delayed timing...
Targeted mutant mice generated on a C57BL/6 background are powerful tools for analysis of the biological functions genes, and gene targeting technologies using mouse embryonic stem (ES) cells have been used to generate such mice. Recently, bacterial artificial chromosome (BAC) recombineering system was established construction vectors. However, retrieval from BACs generation vectors this remains difficult. Even when vector is successful, efficiency production targeted ES derived poor....
Proton single-particle strengths in ${}^{19}\mathrm{F}$ were investigated up to an excitation energy of 14 MeV through the $(d,n)$ reaction on ${}^{18}\mathrm{O}$ at ${E}_{d}=25$ MeV. Spectroscopic deduced from adiabatic-deuteron-breakup-approximation analysis. Most for $2s1d$-shell orbitals observed present measurement. Strength distributions compared with shell-model predictions framework complete basis. Results ${1d}_{5/2}$ and ${2s}_{1/2}$ good agreement calculation, but theoretical...
Abstract Since cervical cancer still afflicts women around the world, it is necessary to understand underlying mechanism of development. Infection with HPV essential for development intraepithelial neoplasia (CIN). In addition, estrogen receptor signaling implicated in cancer. Previously, we have isolated human wings apart-like (WAPL), which expected cause chromosomal instability process HPV-infected precancerous lesions However, role WAPL CIN unknown. this study, order elucidate early...
Abstract Chronic myeloid leukemia (CML) is a myeloproliferative disorder by BCR-ABL, which can transform hematopoietic stem cells (HSCs) into leukemic (LSCs) with limitless capacity for self-renewal. Despite substantial prognostic improvement of CML specific debulking tumor burden tyrosine kinase inhibitor (TKI) targeting ABL kinase, imatinib-treated patients in chronic phase relapse and progress to blast due the remnant cells. Ecotropic viral integration site 1 (EVI1), key transcription...
Epigenetics including DNA methylation is involved in gene silencing and fundamental to embryonic development mammals. Genome‐wide analyses have indicated that there are numerous tissue‐dependent differentially methylated regions (T‐DMRs). Mammals four Spalt‐like genes ( Sall1–4 ), which encode C2H2 type transcription factors indispensable for normal development. We found all the Sall family contained T‐DMRs were hypomethylated stem (ES) cells but hypermethylated trophoblast (TS) cells,...