A5023244715- Acute Myeloid Leukemia Research
- Protein Degradation and Inhibitors
- Chronic Myeloid Leukemia Treatments
- Histone Deacetylase Inhibitors Research
- COVID-19 epidemiological studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Acute Lymphoblastic Leukemia research
- Retinoids in leukemia and cellular processes
- Kruppel-like factors research
- Multiple Myeloma Research and Treatments
- HER2/EGFR in Cancer Research
- Mast cells and histamine
- Blood disorders and treatments
- Neuroblastoma Research and Treatments
- Chromatin Remodeling and Cancer
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Chemokine receptors and signaling
- RNA Research and Splicing
- Cancer-related gene regulation
Chiba Cancer Center
2024-2025
Kyoto University
2013-2023
Kyoto City Hospital
2022
The University of Tokyo
2009-2021
Kyoto University of Education
2020
Matsumoto University
2017
Japan Science and Technology Agency
2012-2014
University of Tokyo Hospital
2014
National Human Genome Research Institute
2007-2011
National Institutes of Health
2009-2011
Runt-related transcription factor 1 (RUNX1) is generally considered to function as a tumor suppressor in the development of leukemia, but growing body evidence suggests that it has pro-oncogenic properties acute myeloid leukemia (AML). Here we have demonstrated antileukemic effect mediated by RUNX1 depletion highly dependent on functional p53-mediated cell death pathway. Increased expression other RUNX family members, including RUNX2 and RUNX3, compensated for antitumor elicited silencing,...
kpm is a human serine/threonine kinase that homologous to Drosophila tumor suppressor warts/lats and its mammalian homologue LATS1. In order define the biological function of kpm, we generated stable transfectants wild-type (kpm-wt), kinase-dead mutant ofkpm (kpm-kd), luciferase in HeLa Tet-Off cells under tetracycline-responsive promoter. Western blot analysis showed high levels expression kpm-wt as well kpm-kd with an apparent mass 150 kDa were induced after removal doxycycline. Induction...
The dual function of runt-related transcriptional factor 1 (RUNX1) as an oncogene or oncosuppressor has been extensively studied in various malignancies, yet its role gastric cancer remains elusive. Up-regulation the ErbB2/HER2 signaling pathway is frequently-encountered and contributes to maintenance these cells. This cascade partly mediated by son sevenless homolog (SOS) family, which adaptor proteins RTK cascades. Herein we report that RUNX1 regulates cells through transactivating SOS1...
Abstract Although runt-related transcription factor 1 (RUNX1) and its associating core binding factor-β (CBFB) play pivotal roles in leukemogenesis, inhibition of RUNX1 has now been widely recognized as a novel strategy for anti-leukemic therapies, it elusive how leukemic cells could acquire the serious resistance against RUNX1-inhibition therapies also whether CBFB participate this process. Here, we show evidence that p53 (TP53) are sequentially up-regulated response to depletion, their...
We have focused on pyrrole-imidazole (PI) polyamide compounds, which preferentially bind to their target DNA sequences. To validate our "CROX (Cluster Regulation of RUNX)" strategy, we created a novel PI polyamide-based inhibitor against RUNX termed Chb-M'. Recently, confirmed its cancer-specific uptake in mouse xenograft derived from HER2-positive gastric cancer cells. The accumulation and efficacy Chb-M' has not yet been investigated vivo, is simpler less expensive method other than...
Abstract Autophagy occurs in human neutrophils after the phagocytosis of multidrug-resistant bacteria and drug-sensitive strains, including Escherichia coli Pseudomonas aeruginosa. The present study detected autophagy by immunoblot analysis LC3B conversion, confocal scanning microscopic examination aggregate formation transmission electron bacteria-containing autophagosomes. Patients with severe bacterial infections are often treated IVIG alongside antimicrobial agents. Here, we showed that...
Multiple myeloma (MM) is an incurable disease. The acquisition of resistance to drugs, including immunomodulatory drugs (IMiDs), has a negative effect on its prognosis. Cereblon (CRBN) key mediator the bioactivities IMiDs such as lenalidomide. Moreover, genetic alteration CRBN frequently detected in IMiD-resistant patients and considered contribute IMiD resistance. Thus, overcoming IMiDs, expected improve clinical outcomes. Here, we examined potential mechanisms histone deacetylase (HDAC)...
Abstract Glioblastoma is the most common adult brain tumour, representing a high degree of malignancy. Transcription factors such as RUNX1 are believed to be involved in malignancy glioblastoma. functions an oncogene or tumour suppressor gene with diverse target genes. Details effects on acquisition glioblastoma remain unclear. Here, we show that downregulates p21 by enhancing expressions BIRC5 and PIF1, conferring anti-apoptotic properties A switch-off therapy using alkylating...
Besides being a classical tumor suppressor, runt-related transcription factor 1 (RUNX1) is now widely recognized for its oncogenic role in the development of acute myeloid leukemia (AML). Here we report that this bidirectional function RUNX1 possibly arises from total level RUNX family expressions. Indeed, analysis clinical data revealed intermediate-level gene expression marked poorest-prognostic cohort relation to AML patients with high- or low-level Through series knockdown experiments...
Mixed-lineage leukemia (MLL) gene rearrangements are among the most frequent chromosomal abnormalities in acute myeloid (AML). MLL fusion patterns associated with patient's prognosis; however, their relationship driver mutations is unclear. We conducted sequence analyses of 338 genes pediatric patients MLL-rearranged (MLL-r) AML (n = 56; JPLSG AML-05 study) alongside data from TARGET study's cohorts MLL-r 104), non-MLL-r 581), and adult 81). KRAS were high-risk fusions (MLL-MLLLT10,...