A5075515862- Mechanisms of cancer metastasis
- Cancer Mechanisms and Therapy
- Plant Genetic and Mutation Studies
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Ferrocene Chemistry and Applications
- Caveolin-1 and cellular processes
- Cancer-related molecular mechanisms research
- Metastasis and carcinoma case studies
- Cancer, Lipids, and Metabolism
- Cell Adhesion Molecules Research
- Vitamin D Research Studies
- Polyamine Metabolism and Applications
- Animal Nutrition and Physiology
- Neonatal Respiratory Health Research
- Glycosylation and Glycoproteins Research
- Growth Hormone and Insulin-like Growth Factors
- Hypothalamic control of reproductive hormones
- Retinoids in leukemia and cellular processes
- Monoclonal and Polyclonal Antibodies Research
- Cholesterol and Lipid Metabolism
- Glioma Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Mesenchymal stem cell research
- Melanoma and MAPK Pathways
University of Kentucky
2005-2023
VA Maryland Health Care System
2021
University of Maryland, Baltimore
2012-2020
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2017-2020
University of Baltimore
2017-2019
Markey Cancer Center
2010-2018
U-M Rogel Cancer Center
2017
University of Mary
2017
The Graduate Center, CUNY
2011
Inserm
2011
Cyclic AMP responsive elements (CRE) have been identified in several genes, including those encoding the a-subunit of glycoprotein hormones and cytosolic form phosphoenolpyruvate carboxykinase (PEPCK) from rat.Common to CRE these genes is palindromic sequence T(G/T)ACGTCA.Based upon strong conservation this element, we hypothesize that functions by binding a protein has conserved across tissue species lines.Scatchard analysis gel mobility shift assays indicate nuclear extracts prepared rat...
The platelet-derived growth factor (PDGF)-A promoter is regulated by a number of GC-rich regulatory elements that possess non-B-form DNA structures. Screening HeLa cDNA expression library with the C-rich strand PDGF-A silencer sequence (5′-S1 nuclease-hypersensitive site (SHS)) yielded three clones encoding NM23-H1, protein implicated as suppressor metastasis in melanoma and breast carcinoma. Recombinant human NM23-H1 cleaved within 3′-portions both 5′-SHS strands either single-stranded or...
The Asn-linked oligosaccharides from bovine lutropin (bLH(Pit] are predominantly dibranched complex-type structures with the terminal sequence SO4-4GalNAc beta 1,4GlcNAc 1,2Man alpha. Recombinant bLH expressed in Chinese hamster ovary cells (bLH(CHO] bears di- (60%) and tribranched (30%) oligosaccharides; however, these terminate Sia alpha 2,3Gal In contrast to limited spectrum of oligosaccharide present on recombinant bLH(CHO), endogenous glycoproteins synthesized by CHO bear a...
NM23-H1 belongs to a family of eight gene products in humans that have been implicated cellular differentiation and development, as well oncogenesis tumor metastasis. We defined biochemically 3′-5′ exonuclease by virtue its ability stoichiometric amounts excise single nucleotides stepwise manner from the 3′ terminus DNA. The activity is dependent upon presence Mg2+, most pronounced with single-stranded substrates or mismatched bases at double-stranded substrates, inhibited both ATP...
ErbB2+ human breast cancer is a major clinical problem. Prior results have suggested that tetraspanin CD151 might contribute to ErbB2-driven growth, survival, and metastasis. In other types, sometimes supports tumor growth However, definitive test of effects on de novo initiation, metastasis has not previously been done. We used gene-deleted mice expressing the MMTV-ErbB2 transgene show strongly mammary initiation Delayed onset (by 70-100 days) in absence was accompanied by reduced survival...
The metastasis suppressor NM23-H1 possesses 3 enzymatic activities in vitro, a nucleoside diphosphate kinase (NDPK), protein histidine and more recently characterized 3'-5' exonuclease. Although the has been implicated suppression of motility breast carcinoma cell lines, potential relevance NDPK exonuclease to function not addressed detail. To this end, site-directed mutagenesis biochemical analyses bacterially expressed mutant proteins have identified mutations that disrupt alone (Glu(5)...
Melanoma is a metastatic cancer associated with poor survival. Here, we study subpopulation of melanoma cells displaying stem cell (MCS cells) properties including elevated expression markers, increased ability to survive as spheroids, and enhanced migration invasion. We show that the Ezh2 survival protein enriched in MCS knockdown or treatment small molecule inhibitors, GSK126 EPZ‐6438, reduces activity. This reduction reduced spheroid formation, migration, Moreover, diet‐derived prevention...
Reduced expression of the metastasis suppressor NM23-H1 is associated with aggressive forms multiple cancers. Here, we establish that (termed H1 isoform in human, M1 mouse) and two its attendant enzymatic activities, 3'-5' exonuclease nucleoside diphosphate kinase, are novel participants cellular response to UV radiation (UVR)-induced DNA damage. deficiency compromised kinetics repair for total polymerase-blocking lesions nucleotide excision (6-4) photoproducts vitro. Kinase activity was...
// Lauren A. Baldwin 1,2,* , John T. Hoff Jason Lefringhouse Michael Zhang 1 Changhe Jia Zeyi Liu Sonia Erfani Hongyan Jin Mei Xu Qing-Bai She R. van Nagell 2 Chi Wang Li Chen Rina Plattner David M. Kaetzel 3 Luo Lu 4 Dava West Chunming Fred Ueland Ronny Drapkin 5 Binhua P. Zhou and Xiuwei H. Yang Department of Pharmacology Nutritional Science, Molecular Cellular Biochemistry, Markey Cancer Center, University Kentucky, Lexington, KY Division Gynecologic Oncology, Obstetrics Gynecology,...
Abstract Melanoma is a lethal skin cancer prone to progression and metastasis, resistant therapy. Metastasis therapy resistance of melanoma other cancers are driven by tumor cell plasticity, largely via acquisition/loss stem-like characteristics transitions between epithelial mesenchymal phenotypes (EMT/MET). NME1 metastasis suppressor gene that inhibits metastatic potential when its expression enforced in cancers. Herein, we have unmasked novel role for as driver growth distinct from...
all trans-retinoic acid (RA) enhances alveolarization in neonates and reinitiates emphysematous adult rat lungs, suggesting that RA may stimulate cell proliferation by upregulating growth factor ligand and/or receptor expression either indirectly or directly acting on RA-responsive genes encoding factors. We report 1,25-dihydroxyvitamin D 3 (Vit D), alone combination, significantly increase [ H]thymidine incorporation cultured fetal postnatal lung fibroblasts ( P < 0.05). The greatest...
Human CG contains an α-subunit, common to the pituitary glycoprotein hormones, and a hormone-specific β-subunit, but unlike βsubunits, hCGβ is characterized by O-glycosylated carboxy-terminal extension. A mutant des-(122–145)hCGβ, was prepared using site-directed mutagenesis, pRSV expression plasmids were transfected into Chinese hamster ovary cells that produce bovine α-subunit (bα). The β-subunit binds bα, heterologous gonadotropin, bα-des-(122–145)hCGβ, capable of stimulating...
Cutaneous malignant melanoma is the most lethal form of skin cancer, with 5-year survival rates <5 % for patients presenting metastatic disease. Mechanisms underlying spread UVR-induced are not well understood, in part due to a paucity animal models that accurately recapitulate disease its advanced forms. We have employed transgenic mouse strain harboring tandem deletion nm23-m1 and nm23-m2 genes assess combined contribution these suppression metastasis. Crossing nm23-h1/nm23-h2 knockout...
Biologically active bovine luteinizing hormone (LH) has been obtained through expression of the alpha- and LH beta-subunit genes in stably transformed clones DUXB11, a Chinese hamster ovary cell line deficient dihydrofolate reductase (DHFR). Expression alpha-and mRNAs expected sizes (approximately 910 770 nucleotides, respectively) were revealed by blot analysis after electrophoresis total cellular RNA. Furthermore, presence or absence gonadotropin several clonal lines was directly...
Abstract A complex profile of gene expression elicited by autocrine platelet-derived growth factor (PDGF) signaling was identified in U87 MG glioblastoma cells microarray analysis. The most striking pattern observed a PDGF-dependent activation at least 25 genes involved with biosynthesis and/or uptake cholesterol and isoprenoids, including mevalonate pyrophosphate decarboxylase, 3-hydroxy-3-methylglutaryl coenzyme (HMG-CoA) synthase, HMG-CoA reductase, low-density lipoprotein receptor....