A5070686533- Liver Disease Diagnosis and Treatment
- Nanoparticles: synthesis and applications
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Immune cells in cancer
- Liver Disease and Transplantation
- Effects and risks of endocrine disrupting chemicals
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Advanced Proteomics Techniques and Applications
- Air Quality and Health Impacts
- Hepatitis C virus research
- Advanced biosensing and bioanalysis techniques
- biodegradable polymer synthesis and properties
- 3D Printing in Biomedical Research
- Bariatric Surgery and Outcomes
- Graphene and Nanomaterials Applications
- S100 Proteins and Annexins
- Metabolomics and Mass Spectrometry Studies
- Immune Response and Inflammation
- Computational Drug Discovery Methods
- Fibroblast Growth Factor Research
- Heavy Metal Exposure and Toxicity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nanopore and Nanochannel Transport Studies
Helmholtz Centre for Environmental Research
2019-2025
Innsbruck Medical University
2023
Universität Innsbruck
2023
ORCID
2021
University of Bucharest
2020
Federal Institute for Risk Assessment
2020
BASF (United States)
2020
University of Namur
2020
Extibacter muris is a newly described mouse gut bacterium which metabolizes cholic acid (CA) to deoxycholic (DCA) via 7α-dehydroxylation. Although bile acids influence metabolic and inflammatory responses, few in vivo models exist for studying their metabolism impact on the host. Mice were colonized from birth with simplified community Oligo-MM12 or without E. muris. As of known affect lipid homeostasis, mice fed either low- high-fat diet eight weeks before sampling analyses targeting liver....
Abstract Parabens are preservatives widely used in consumer products including cosmetics and food. Whether low-dose paraben exposure may cause adverse health effects has been discussed controversially recent years. Here we investigate the effect of prenatal on childhood overweight by combining epidemiological data from a mother–child cohort with experimental approaches. Mothers reporting use paraben-containing cosmetic have elevated urinary concentrations. For butyl (BuP) positive...
The obesity pandemic is presumed to be accelerated by endocrine disruptors such as phthalate-plasticizers, which interfere with adipose tissue function. With the restriction of plasticizer di-(2-ethylhexyl)-phthalate (DEHP), search for safe substitutes gained importance. Focusing on master regulator adipogenesis and functionality, peroxisome proliferator-activated receptor gamma (PPARγ), we evaluated 20 alternative plasticizers well their metabolites binding activation PPARγ assessed effects...
The danger signal extracellular calcium is pathophysiologically increased in the synovial fluid of patients with rheumatoid arthritis (RA). Calcium activates NLRP3-inflammasome via calcium-sensing receptor monocytes/macrophages primed by lipopolysaccharide, and this effect mediated uptake calciprotein particles (CPPs) formed out calcium, phosphate, fetuin-A. Aim study was to unravel influence on monocytes when priming not present. Monocytes were isolated from blood healthy controls RA...
Bisphenol A (BPA), which is used in a variety of consumer-related plastic products, was reported to cause adverse effects, including disruption adipocyte differentiation, interference with obesity mechanisms, and impairment insulin- glucose homeostasis. Substitute compounds are increasingly emerging but not sufficiently investigated.We aimed investigate the mode action BPA four its substitutes during differentiation human preadipocytes adipocytes their molecular interaction peroxisome...
Emerging studies revealed that the Aryl hydrocarbon receptor (AhR), a sensing environmental contaminants, is executing an immunomodulatory function. However, it open question to which extent this achieved by its role as transcription factor or via non-genomic signaling. We utilized multi-post-translational modification-omics approach examine AhR-signaling after activation with endogenous (FICZ) exogenous (BaP) ligand in endotoxin-activated (LPS) monocyte-derived macrophages. While AhR...
Since hepatocytes produce majority of serum proteins, patients with cirrhosis display substantial alterations in the proteome. The aim current study was to characterize these changes and prognostic utility hepatocellular proteins available routine clinical testing.Sera from 29 healthy controls 43 were subjected untargeted proteomic analysis. Unsupervised hierarchical clustering performed Perseus software R. Ingenuity pathway analysis (IPA) suggested upstream regulators that validated liver...
In respect to the high number of released nanomaterials and their highly variable properties, novel grouping approaches are required based on effects nanomaterials. Proper calls for a combination an experimental setup with higher structurally similar employing integrated omics identify mode action. Here, we analyzed seven well-characterized NMs comprising different chemical compositions, sizes surface modifications rat alveolar macrophage cell line NR8383. The were investigated at three...
The use of the plasticizer bis(2-ethylhexyl)phthalate (DEHP) and other plasticizers in manufacture plastic products has been restricted due to adverse health outcomes such as obesity, metabolic syndrome, asthma, for which inflammation described be a driving factor. emerging alternative 1,2-cyclohexanedioic acid diisononyl ester (DINCH) still lacks information regarding its potential effects on immune system. Here, we investigated DINCH naturally occurring metabolite...
Abstract Background Nanomaterials (NMs) can be fine-tuned in their properties resulting a high number of variants, each requiring thorough safety assessment. Grouping and categorization approaches that would reduce the amount testing are principle existing for NMs but still mostly conceptual. One drawback is limited mechanistic understanding NM toxicity. Thus, we conducted multi-omics vitro study RLE-6TN rat alveolar epithelial cells involving 12 covering different materials including...
Human white adipose tissue (AT) is a metabolically active organ with distinct depot-specific functions. Despite their locations close to the gastrointestinal tract, mesenteric AT and epiploic (epiAT) have only scarcely been investigated. Here, we aim characterise these ATs in-depth estimate contribution alterations in whole-body metabolism.Mesenteric, epiploic, omental abdominal subcutaneous were collected from 70 patients obesity undergoing Roux-en-Y gastric bypass surgery. The...
The application of quantitative proteomics provides a new and promising tool for standardized toxicological research. However, choosing suitable method still puzzles many researchers because the optimal needs to be determined. In this study, we investigated advantages limitations two most commonly used global methods, namely label-free quantitation (LFQ) tandem mass tags (TMT). As case exposed hepatocytes (HepG2) environmental contaminant benzo[a]pyrene (BaP) using concentration 2 μM. Our...
Mesenchymal stromal cell (MSC) transplantation ameliorated hepatic lipid load; tissue inflammation; and fibrosis in rodent animal models of non-alcoholic steatohepatitis (NASH) by as yet largely unknown mechanism(s). In a mouse model NASH; we transplanted bone marrow-derived MSCs into the livers; which were analyzed one week thereafter. Combined metabolomic proteomic data applied to weighted gene correlation network analysis (WGCNA) subsequent identification key drivers. Livers...
The immense diversity and constant development of nanomaterials (NMs) increase the need for a facilitated risk assessment, which requires knowledge modes action (MoAs) NMs. This necessitates comprehensive data basis, can be obtained using omics. Furthermore, establishment suitable in vitro test systems is essential to follow 3R concept cope with high number In present study, we aimed compare NM effects vivo multi-omics approach. We applied an integrated analysis strategy based on proteomics...
The function of mucosal-associated invariant T (MAIT) cells highly depends on the mode activation, either by recognition bacterial metabolites via their cell receptor (TCR) or in a TCR-independent manner cytokines. underlying molecular mechanisms are not entirely understood. To define activation MAIT level, we applied multi-omics approach with untargeted transcriptomics, proteomics and metabolomics. Transcriptomic analysis E. coli- TCR-activated showed distinct transcriptional reprogramming,...
An equilibrium between proinflammatory and anti-inflammatory immune responses is essential for maternal tolerance of the fetus throughout gestation. To study participation fetal tissue-derived factors in this delicate balance, we analyzed effects human chorionic gonadotropin (hCG) on murine Treg cells Th17 vitro , pregnancy outcomes, placental growth, blood flow velocities remodeling uterine vascular bed vivo . Compared with untreated CD4 + CD25 T cells, hCG increased frequency upon...
Sensing microbial tryptophan catabolites by the aryl hydrocarbon receptor (AhR) plays a pivotal role in host-microbiome homeostasis modulating host immune response. Nevertheless, involved cellular processes triggered metabolites are mainly unknown. Here, we analyzed proteomic changes macrophages after treatment with indole-3-acetic acid (I3AA) or indole-3-aldehyde (IAld), as well prototypic exogenous AhR-ligand benzo(a)pyrene (BaP) absence and presence of lipopolysaccharide (LPS) to identify...
A bstract Tumor-associated macrophages (TAM) enhance cancer progression by promoting angiogenesis, extracellular matrix (ECM) remodeling, and immune suppression. Nerve infiltration is a hallmark of various cancers known to directly contribute tumor growth. However, the role TAM in intratumoral nerve growth remains poorly understood. In this study, we demonstrate that expressed distinct “neural growth” gene signature. actively neural within tumors promote neurites outgrowth. We identify...
Nanomaterials (NMs) taken up from the environment carry a complex ecocorona consisting of dissolved organic matter. An is assumed to influence interactions between NMs and endogenous biomolecules consequently affects formation biological corona (biocorona) fate NMs. This study shows that in fish plasma attach immediately (within <5 min) surface SWCNTs evolution biocorona size dependent phenomenon. Quantitative proteomics data revealed nanotube also influences protein composition on SWCNTs....
Although macrophages are known to be affected by their redox status, oxidation is not yet a well-recognized post-translational modification (PTM) in regulating and immune cells general. While it has been described that the status of single cysteines specific proteins relevant for macrophage functions, global information scarce. Hence, we globally assessed impact on activation using untargeted proteomics PTM-omics. We exposed THP-1 lipopolysaccharide (LPS) 4 h 24 applied sequential iodoTMT...