A5031383668- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- HVDC Systems and Fault Protection
University of Michigan
2022-2024
While the centrality of posttranscriptional modifications to RNA biology has long been acknowledged, function vast majority modified sites remains be discovered. Illustrative this, there is not yet a discrete biological role assigned for one most highly conserved modifications, 5-methyluridine at position 54 in tRNAs (m
Significance Pseudouridine is among the most-abundant RNA modifications. We present a framework for conceptualizing how eukaryotic pseudouridine synthases select their substrates. This work reveals structure of yeast synthase 7 (Pus7) and presents cell-based biochemical investigations enzyme binding activity. demonstrate that Pus7 interacts promiscuously with RNAs containing UG U AR sequences. Our observations raise question why these enzymes only modify <5% sequences in transcriptome,...