Abstract Modified interleukin-2 (IL-2) formulations are being tested in cancer patients. However, IL-2 immunotherapy damages receptor (IL-2R)-positive endothelial cells and stimulates IL-2Rα (CD25)-expressing lymphocytes that curtail anti-tumor responses. A first generation of IL-2Rβ (CD122)-biased IL-2s addressed some these drawbacks. Here, we present a second-generation CD122-biased IL-2, developed by splitting permanently grafting unmutated human (hIL-2) to its antigen-binding groove on...

Aims: Little is known about the effects of phytochemicals against Borrelia sp.causing Lyme disease.Current therapeutic approach to this disease limited antibiotics.This study examined anti-borreliae efficacy several plantderived compounds and micronutrients.Methods Results: We tested 15 micronutrients three morphological forms burgdoferi garinii: spirochetes, latent rounded biofilm.The results showed that most potent substances spirochete B. burgdorferi garinii were cis-2-decenoic acid,...

Although fully treatable in the early stages, once cervical cancer has metastasized, patient outcome is poor. The main objective of this study was to examine effect dietary supplementation with a nutrient mixture (NM) containing lysine, ascorbic acid, proline, green tea extract and other micronutrients on HeLa cell xenografts nude female mice. Tumor growth measured immunohistochemical staining evaluated for following markers: Ki67 (proliferation); matrix metalloproteinase (MMP)-2 -9...

Borrelia sp. is a causative pathogen of Lyme disease which has become worldwide health concern. Non-toxic approaches especially directed toward latent persistent forms this are desired. Lipids in the form volatile and non-volatile oils, fatty acids with proven anti-borreliae efficacy could an additional support or alternative for consideration treatment approaches. In study we investigated 47 lipids (30 17 acids) plant animal origin against typical motile, knob/round-shaped persisters,...

ABSTRACT For animals to survive until reproduction, it is crucial that juveniles successfully detect potential predators and respond with appropriate behavior. The recognition of cues originating from can be innate or learned. Cues various modalities might used alone in multi-modal combinations distinguish but studies investigating integration predator avoidance are scarce. Here, we wild, naive tadpoles the Neotropical poison frog Allobates femoralis ( Boulenger, 1884) test their reaction...

Abstract Aims: Based on the poor prognosis associated with ovarian cancer and reported anticancer properties of specific nutrients, we investigated effect a nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid epigallocatechin gallate human cells SK‐OV‐3 by measuring: cell proliferation, modulation matrix metalloproteinase (MMP)‐2 ‐9 expression, invasive potential. Methods: Cell proliferation was evaluated MTT assay, MMP activity gelatinase zymography, invasion through...

The role of PEG-coated gold nanoparticles (Au NPs) on the anti-proliferative effect Specific Nutrient Synergy (SNS) HTLV-1 infected (C91-PL and HuT-102) non-infected (CEM Jurkat) malignant T-lymphocytes cells, was investigated. When Au NPs (of different molecular weights) were added alone, there no either viability or proliferation leukemic cell lines studied. Treatment cells with SNS PEG (5 10 kDa) coated NP reduced significantly in all tested; this reached more than 50% reduction as...

Introduction: Matrix metalloproteinases (MMPs) have received much attention in recent years for their role various malignancies, and been implicated tumor invasion, metastasis angiogenesis. Certain MMPs, such as MMP-2 MMP-9, shown a special propensity promoting cancer. Development of MMP inhibitors has approach to controlling cancer blocking metastasis. Objective: We investigated the effect EGCG individually combination with lysine, proline, ascorbic acid (LPA), vitro, on human fibrosarcoma...

Phytochemicals and micronutrients represent a growing theme in antimicrobial defense; however, little is known about their anti-borreliae effects of reciprocal cooperation with antibiotics.A better understanding this aspect could advance our knowledge help improve the efficacy current approaches towards Borrelia sp.In study, phytochemicals such as baicalein, luteolin, 10-HAD, iodine, rosmarinic acid, monolaurin, well as, vitamins D3 C were tested combinations doxycycline for vitro...

Psoriasis is a chronic inflammatory skin disease characterized by thickened, silvery-scaled patches. There currently no cure and treatments only attempt to reduce the severity of symptoms. This study reports case 36-year-old female who presented clinic with severe psoriasis had been treated topical steroid cream for past 14 years. After adherence prescribed dietary changes 6 months, including abundant intake vegetables, minimal consumption meat, avoidance junk food sugar in or drinks, as...

In the search for novel agents against oral pathogens in their planktonic and biofilm form, we have focused our attention on 10-undecynoic acid as representative of acetylenic fatty acids. Using macro-broth susceptibility testing method first established MIC value. Next, MBC value was determined from a broth dilution minimum inhibitory concentration test by sub-culturing it to BHI agar plates that did not contain agent. Anti-biofilm efficacy tested 96-well coated with saliva using...

The dysregulation of renin-angiotensin system-signaling is a contributing factor to the development atherosclerosis and cardiovascular disease. We studied progression angiotensin II (AngII)-induced arterial wall after its induction. Atherosclerosis was accelerated in ApoE (-/-) C57B6 mice by 4 weeks continuous osmotic pump administration AngII (23 nmol/24 h). Changes aortae were evaluated these at 16 installation, as well control that not administered AngII. In comparison non-AngII controls,...

The cases of Lyme disease caused by Borrelia burgdorferi infection have been increasing throughout Northern America and Europe. This pathogen, if not treated in a timely manner with antibiotics, can cause persisting debilitating health outcomes. In the search for novel agents against B. burgdorferi, we investigated phenolic compound-gallic acid-for its anti-Borrelia anti-inflammatory effects. Our results showed biocidal effect starting from 100 μg/mL active spirochetes,...

Borrelia sp., a causative pathogenic factor of Lyme disease (LD), has become major public health threat. Current treatments based on antibiotics often lead to relapse after their withdrawal. Naturally derived substances that could work synergistically display higher efficacy compared with the individual components may serve as resource for development novel approaches combat both active and latent forms sp.Using checkerboard assay, we investigated anti-borreliae reciprocal cooperation...

Lyme disease (LD) is a tick-borne infection caused by Borrelia burgdorferi sensu lato. The current therapeutic approach to this limited antibiotics. However, after their administration, about 20% of patients experience delayed onset illness manifesting as lingering persistent symptoms.To determine suitable that would help reduce number, we examined the efficacy composition polyphenolic compounds (baicalein, luteolin, and rosmarinic acid) with fatty acids (monolaurin cis-2-decenoic acid),...

is a major pathogenic habitant of oral caries. Owing to its physiological and biochemical features, it prevails in the form plaque biofilm together with another important mutans streptococci species,