A5046884070- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Tea Polyphenols and Effects
- Plant nutrient uptake and metabolism
- Plant biochemistry and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Food Quality and Safety Studies
- Transgenic Plants and Applications
- Soybean genetics and cultivation
- Molecular Biology Techniques and Applications
- Plant Reproductive Biology
- Light effects on plants
- Genetics and Plant Breeding
- Botanical Research and Chemistry
- Biological Activity of Diterpenoids and Biflavonoids
- RNA and protein synthesis mechanisms
- Plant and Biological Electrophysiology Studies
- Legume Nitrogen Fixing Symbiosis
Guizhou University
2023-2025
Third Xiangya Hospital
2021-2025
Central South University
2020-2025
University of North Texas
2022-2024
University of Georgia
2012-2024
Fujian Medical University
2017-2024
University of North Texas Health Science Center
2022-2024
Mengchao Hepatobiliary Hospital
2023-2024
Applied Genetic Technologies (United States)
2024
National Institutes for Food and Drug Control
2022-2023
MCScan is an algorithm able to scan multiple genomes or subgenomes in order identify putative homologous chromosomal regions, and align these regions using genes as anchors. The MCScanX toolkit implements adjusted for detection of synteny collinearity that extends the original software by incorporating 14 utility programs visualization results additional downstream analyses. Applications several sequenced plant gene families are shown examples. can be used effectively analyze chromosome...
Completion of genome sequences for the diploid Setaria italica reveals features C4 photosynthesis that could enable improvement polyploid biofuel crop switchgrass (Panicum virgatum). The genetic basis biotechnologically relevant traits, including drought tolerance, photosynthetic efficiency and flowering control, is also highlighted. We generated a high-quality reference sequence foxtail millet (Setaria italica). ∼400-Mb assembly covers ∼80% >95% gene space. was anchored to 992-locus map...
Simple sequence repeats (SSRs), also referred to as microsatellites, are highly variable tandem DNAs that widely used genetic markers. The increasing availability of whole-genome and transcript sequences provides information resources for SSR marker development. However, efficient software is required efficiently identify display along with other gene features at a genome scale. We developed novel package Genome-wide Microsatellite Analyzing Tool Package (GMATA) integrating mining,...
Abstract Teff ( Eragrostis tef ) is a cornerstone of food security in the Horn Africa, where it prized for stress resilience, grain nutrition, and market value. Here, we report chromosome-scale assembly allotetraploid teff (variety Dabbi) patterns subgenome dynamics. The genome contains two complete sets homoeologous chromosomes, with most genes maintaining as syntenic gene pairs. TE analysis allows us to estimate that polyploidy event occurred ~1.1 million years ago (mya) subgenomes...
Root growth control plays an important role in plant adaptation to drought stress, but the underlying molecular mechanisms of this remain largely elusive. Here, a root-specific nuclear factor Y (NF-Y) transcription PdNF-YB21 was isolated from Populus. The functional mechanism characterised by various morphological, physiological, molecular, biochemical and spectroscopy techniques. Overexpression poplar promoted root with highly lignified enlarged xylem vessels, resulting increased...
Low temperature restricts the planting range of all crops, but cold acclimation induces adaption to stress in many plants. Camellia sinensis, a perennial evergreen tree that is source tea, mainly grown warm areas. sinensis var. (CSS) has greater tolerance than assamica (CSA). To gain deep insight into molecular mechanisms underlying adaptation, we investigated physiological responses and transcriptome profiles by RNA-Seq two tea varieties, resistant SCZ (classified as CSS) susceptible YH9...
Oxylipins, including jasmonic acid (JA) and volatiles, are important for signaling in plants, these formed by the lipoxygenase (LOX) enzyme family. There is a large gap understanding of underlying molecular basis their roles tea plants. Here, we identified 11 CsLOX genes from plant (Camellia sinensis), characterized phylogeny, gene structure protein features into three subclasses. We then examined enzymatic activities, LOX expression alternative splicing transcripts during development...
Applied nitrogen (N) fertilizer significantly increases the leaf yield. However, most N is not utilized by plant, negatively impacting environment. To date, little known regarding utilization genes and mechanisms in production. understand this, we investigated transcriptomes using RNA-seq amino acid levels with treatment tea (Camellia sinensis), popular beverage crop. We identified 196 29 common differentially expressed roots leaves, respectively, response to ammonium two varieties. Among...
Background Type 2 diabetes mellitus (T2DM) was a major global health threat. As chronic low-grade inflammatory disease, the prognosis of associated with inflammation. The advanced lung cancer inflammation index (ALI) served as comprehensive to assess This study aimed estimate association between ALI and all-cause, cardiovascular disease (CVD), mortality in T2DM patients. Methods We extracted cohort data from National Health Nutrition Examination Survey (NHANES) spanning 1999-2018 for...
Abstract Finger millet is a key food security crop widely grown in eastern Africa, India and Nepal. Long considered ‘poor man’s crop’, finger has regained attention over the past decade for its climate resilience nutritional qualities of grain. To bring breeding into 21 st century, here we present assembly annotation chromosome-scale reference genome. We show that this ~1.3 million years old allotetraploid high level homoeologous gene retention lacks subgenome dominance. Population structure...
Simple Sequence Repeats (SSR), also called microsatellite, is very useful for genetic marker development and genome application. The increasing whole sequences of more large genomes provide sources SSR mining in silico. However currently existing tools can't process efficiently generate no or poor statistics. Genome-wide Microsatellite Analyzing Tool (GMATo) a novel tool statistics at aspects. It faster accurate than existed Locator MISA. If DNA sequence was too long, it chunked to short...
We report the first study on genetic control of flowering in Setaria, a panicoid grass closely related to switchgrass, and same subfamily as maize sorghum. A recombinant inbred line mapping population derived from cross between domesticated Setaria italica (foxtail millet) its wild relative viridis (green millet), was grown eight trials with varying environmental conditions identify small number quantitative trait loci (QTL) that differences time. Many QTL across colocalize, suggesting is...
Eucommia ulmoides, also called hardy rubber tree, is an economically important tree; however, the lack of its genome sequence restricts fundamental biological research and applied studies this plant species. Here, we present a high-quality assembly ∼1.2-Gb (scaffold N50 = 1.88 Mb) with at least 26 723 predicted genes for E. first sequenced order Garryales, which was obtained using integrated strategy combining Illumina sequencing, PacBio BioNano mapping. As sister taxon to lamiids...
Tea, made from leaves of Camellia sinensis, has long been consumed worldwide for its unique taste and aroma. Terpenoids play important roles not only in tea beverage aroma formation, but also the productivity quality plantation due to their significant contribution light harvesting pigments phytohormones. To date, however, regulation terpenoid synthase genes remains unclear. Herein, analyses metabolomics, sRNAs, degradome, transcriptomics were performed integrated identifying key regulatory...
Research on orphan crops is often hindered by a lack of genomic resources. With the advent affordable sequencing technologies, genotyping an entire genome or, for large-genome species, representative fraction has become feasible any crop. Nevertheless, most genotyping-by-sequencing (GBS) methods are geared towards obtaining large numbers markers at low sequence depth, which excludes their application in heterozygous individuals. Furthermore, bioinformatics pipelines flexibility to deal with...
Herbivore-induced plant volatiles (HIPVs) play important ecological roles in defense against stresses. In contrast to model plants, reports on HIPV formation and function crops are limited. Tea (Camellia sinensis) is an crop China. α-Farnesene a common produced tea plants response different herbivore attacks. this study, C. sinensis α-farnesene synthase (CsAFS) was isolated, cloned, sequenced, functionally characterized. The CsAFS recombinant protein Escherichia coli able transform farnesyl...
ABSTRACT We conducted a comprehensive analysis of the total microbiome and transcriptionally active communities in roots root nodules Prosopis cineraria , an important leguminous tree arid regions many Asian countries. Mature P. trees growing desert did not exhibit any detected nodules. However, we observed on farm young plants growth chamber, when inoculated with rhizosphere soil, including soil from near that had no Compared to nearby non-nodulated were enriched Actinobacteria (e.g.,...
Cold environment is the main constraint for tea plants (Camellia sinensis) distribution and farming. We identified two cultivars, called var. sinensis cv. Shuchazao (SCZ) with a high cold-tolerance assamica Yinghong9 (YH9) low cold-tolerance. To better understand response mechanism of under cold stress improving breeding, we compared physiological biochemical responses, associated genes expression in to 7-day 14-day acclimation, followed by de-acclimation these cultivars. found that EL50,...
Simple sequence repeats (SSRs) are tandem of DNA that have been used to develop robust genetic markers. These molecular markers powerful tools for basic and applied studies such as breeding. In the model plants in Nicotiana genus e.g. N. benthamiana, a comprehensive assessment SSR content has become possible now because several genomes sequenced. We conducted genome-wide characterization marker development across seven genomes.Here, we initially characterized 2,483,032 SSRs (repeat units...
Anthracnose disease is caused by Colletotrichum gloeosporioides, and common in leaves of the tea plant (Camellia sinensis). MicroRNAs (miRNAs) have been known as key modulators gene expression response to environmental stresses, resistance, defense responses, immunity. However, role miRNAs responses C. gloeosporioides remains unexplored plant. Therefore, present study, six miRNA sequencing data sets two degradome were generated from gloeosporioides-inoculated control leaves. A total 485...
Alternative splicing (AS) regulates mRNA at the post-transcriptional level to change gene function in organisms. However, little is known about AS and its roles tea plant (Camellia sinensis), widely cultivated for making a popular beverage tea.In our study, landscape dynamics were characterized eight tissues (bud, young leaf, summer mature winter old stem, root, flower, fruit) of by Illumina RNA-Seq confirmed Iso-Seq. The most abundant (~ 20%) was intron retention involved RNA processes....