Martí Ninot‐Pedrosa
A5049182978- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Alzheimer's disease research and treatments
- Animal Virus Infections Studies
- Supramolecular Self-Assembly in Materials
- Virus-based gene therapy research
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Drug Transport and Resistance Mechanisms
- 14-3-3 protein interactions
- Lipid Membrane Structure and Behavior
Université Claude Bernard Lyon 1
2021-2024
Centre National de la Recherche Scientifique
2021-2024
Institute for Research in Biomedicine
2016-2018
Significance Numerous reports indicate that amyloid-β peptide (Aβ) oligomers, considered the pathogenic molecular form of Aβ in Alzheimer´s disease (AD), exert their neurotoxicity within membrane. Therefore, it is critical to characterize them such an environment. Here, we worked with two major variants and handled as if they were membrane proteins. By doing so, found variant most strongly linked AD assembled into stable oligomers adopted a specific structure incorporated membranes pores,...
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands redirection of scientific efforts criteria organized research projects. international COVID19-NMR consortium seeks provide such new approaches gathering expertise worldwide. In particular, making available viral proteins RNAs will pave way understanding molecular components in detail. resources provided through are fully disclosed accelerate access exploitation. NMR...
SARS-CoV-2 (SCoV2) and its variants of concern pose serious challenges to the public health. The increased vaccines, thus necessitating for development new intervention strategies including anti-virals. Within international Covid19-NMR consortium, we have identified binders targeting RNA genome SCoV2. We established protocols production NMR characterization more than 80 % all SCoV2 proteins. Here, performed an screening using a fragment library binding 25 proteins hits also against...
SARS-CoV-2, the virus responsible for COVID-19 pandemic, encodes several accessory proteins, among which ORF6, a potent interferon inhibitor, is recognized as one of most cytotoxic. Here, we investigated structure, oligomeric state, and membrane interactions ORF6 using NMR spectroscopy molecular dynamics simulations. Using chemical-shift-ROSETTA, show that in proteoliposomes adopts straight α-helical structure with an extended, rigid N-terminal part flexible C-terminal residues....
Abstract ORF7b is an accessory protein of SARS-CoV-2, the virus behind COVID-19 pandemic. Using cell-free synthesized ORF7b, we experimentally show that assembles into stable multimers. The sequence shows a transmembrane segment, which multimerizes through leucine zipper. We hypothesize has potential to interfere with important cellular processes involve leucine-zipper formation, and present two particularly striking examples. First, zippers are central in heart rhythm regulation...
Abstract SARS‐CoV‐2 (SCoV2) and its variants of concern pose serious challenges to the public health. The increased vaccines, thus necessitating for development new intervention strategies including anti‐virals. Within international Covid19‐NMR consortium, we have identified binders targeting RNA genome SCoV2. We established protocols production NMR characterization more than 80 % all SCoV2 proteins. Here, performed an screening using a fragment library binding 25 proteins hits also against...
SARS-CoV-2 carries a sizeable number of proteins that are accessory to replication but may be essential for virus–host interactions and modulation the host immune response. Here, we investigated structure largely unknown ORF7b, small membranous membrane protein SARS-CoV-2. We show structural predictions indicate transmembrane (TM) leucine zipper experimentally confirm predominantly α-helical secondary within phospholipid mimetic by solid-state NMR. also ORF7b forms heterogeneous higher-order...
Background: The aggregation of the amyloid-beta peptide (Aβ) in brain is strongly associated with Alzheimer´s disease (AD). However, heterogeneous and transient nature this process has prevented identification exact molecular form Aβ responsible for neurotoxicity observed disease. Therefore, characterizing utmost importance field AD. Nuclear magnetic resonance spectroscopy (NMR) a technique that holds great potential to achieve goal. it requires use specific labels introduced through...