A5049013633- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virology and Viral Diseases
- Malaria Research and Control
- HIV Research and Treatment
- Viral Infections and Outbreaks Research
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Diet and metabolism studies
- Science and Education Research
- SARS-CoV-2 and COVID-19 Research
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- Herpesvirus Infections and Treatments
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Metabolism and Genetic Disorders
- Plant Virus Research Studies
- COVID-19 Clinical Research Studies
- Hemoglobin structure and function
- Biochemical Acid Research Studies
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
Universidade Federal do Rio de Janeiro
2016-2025
National Education and Research Network
2008-2022
National Institute of Science and Technology for Structural Biology and Bioimaging
2020-2021
In-Q-Tel
2010
University of Illinois Urbana-Champaign
1993
Dengue virus is responsible for the highest rates of disease and mortality among members Flavivirus genus. epidemics are still occurring around world, indicating an urgent need prophylactic vaccines antivirals. In recent years, a great deal has been learned about mechanisms dengue genome amplification. However, little known process by which capsid protein recruits viral during encapsidation. Here, we found that mature in cytoplasm infected cells accumulates on surface ER-derived organelles...
Abstract Neurological complications affecting the central nervous system have been reported in adult patients infected by Zika virus (ZIKV) but underlying mechanisms remain unknown. Here, we report that ZIKV replicates human and mouse brain tissue, targeting mature neurons. preferentially targets memory-related regions, inhibits hippocampal long-term potentiation induces memory impairment mice. TNF-α upregulation, microgliosis upregulation of complement proteins, C1q C3, are induced...
Cognitive dysfunction is often reported in patients with post-coronavirus disease 2019 (COVID-19) syndrome, but its underlying mechanisms are not completely understood. Evidence suggests that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein or fragments released from cells during infection, reaching different tissues, including the CNS, irrespective of presence viral RNA. Here, we demonstrate brain infusion mice has a late impact on cognitive function,...
3-BrPA (3-bromopyruvate) is an alkylating agent with anti-tumoral activity on hepatocellular carcinoma. This compound inhibits cellular ATP production owing to its action glycolysis and oxidative phosphorylation; however, the specific metabolic steps mechanisms of in human carcinomas, particularly effects mitochondrial energetics, are poorly understood. In present study it was found that incubation HepG2 cells a low concentration for short period (150 μM 30 min) significantly affected both...
Dengue virus (DENV) affects millions of people, causing more than 20,000 deaths annually. No effective treatment for the disease caused by DENV infection is currently available, partially due to lack knowledge on basic aspects viral life cycle, including molecular basis interaction between components and cellular compartments. Here, we characterized properties capsid (C) protein hepatic lipid droplets (LDs), which was recently shown be essential replication cycle. Zeta potential analysis...
Infection by Zika virus triggers epileptic seizures in newborn mice and leads to viral persistence the brain behavioral deficits adulthood.
Dengue fever is an emerging viral disease transmitted by arthropods to humans in tropical countries. hemorrhagic (DHF) escalating frequency and mortality rates. Here we studied the involvement of macrophage migration inhibitory factor (MIF) dengue virus (DENV) infection its pathogenesis. Patients with DHF had elevated plasma concentrations MIF. Both leukocytes from these patients macrophages healthy donors infected vitro DENV showed a substantial amount MIF within lipid droplets. The...
Dengue is the major arthropod-borne human viral disease, for which no vaccine or specific treatment available. We used NMR, zeta potential measurements and atomic force microscopy to study structural features of interaction between dengue virus C (capsid) protein LDs (lipid droplets), organelles crucial infectious particle formation. protein-binding sites LD were mapped, revealing a new function conserved segment in N-terminal disordered region indicating that conformational selection...
Infection of primary CD4+ T cells with HIV-1 coincides an increase in glycolysis. We investigated the expression glucose transporters (GLUT) and glycolytic enzymes human response to infection HIV-1. demonstrate co-expression GLUT1, GLUT3, GLUT4, GLUT6 after activation, their concerted overexpression infected cells. The investigation demonstrated activation-dependent hexokinases HK1 HK2 cells, a highly significant cellular hexokinase enzyme activity showed marked HK1, as well functionally...
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands redirection of scientific efforts criteria organized research projects. international COVID19-NMR consortium seeks provide such new approaches gathering expertise worldwide. In particular, making available viral proteins RNAs will pave way understanding molecular components in detail. resources provided through are fully disclosed accelerate access exploitation. NMR...
Exacerbated inflammatory response and altered vascular function are hallmarks of dengue disease. Reactive oxygen species (ROS) production has been associated to endothelial barrier disturbance microvascular alteration in distinct pathological conditions. Increased ROS reported vitro models virus (DENV) infection, but its impact for cell physiology had not fully investigated. Our group previously demonstrated that infection human brain cells (HBMEC) with DENV results the activation RNA...
ABSTRACT Vesicular stomatitis virus (VSV) infection depends on the fusion of viral and cellular membranes, which is mediated by spike glycoprotein G at acidic environment endosomal compartment. VSV protein does not contain a hydrophobic amino acid sequence similar to peptides found among other glycoproteins, suggesting that membrane recognition occurs through an alternative mechanism. Here we studied interaction between liposomes different phospholipid composition force spectroscopy,...
Dengue virus (DENV), a mosquito-borne flavivirus, is public health problem in many tropical countries. IL-22 and IL-17A are key cytokines several infectious inflammatory diseases. We have assessed the contribution of pathogenesis experimental dengue infection using mouse-adapted DENV serotype 2 strain (P23085) that causes disease resembles severe humans. show produced upon DENV-2 immune-competent mice. Infected IL-22(-/-) mice had increased lethality, neutrophil accumulation pro-inflammatory...
Dengue virus (DENV) infection affects millions of people and is becoming a major global disease for which there no specific available treatment. pep14-23 recently designed peptide, based on conserved segment DENV capsid (C) protein. It inhibits the interaction C with host intracellular lipid droplets (LDs), crucial viral replication. Combining bioinformatics biophysics, here, we analyzed structure ability to bind different phospholipids, relating that information full-length C. We show...