A5067353910- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Advanced Nanomaterials in Catalysis
- Viral Infectious Diseases and Gene Expression in Insects
- Dendrimers and Hyperbranched Polymers
- Gold and Silver Nanoparticles Synthesis and Applications
- Viral gastroenteritis research and epidemiology
- MicroRNA in disease regulation
- Radiopharmaceutical Chemistry and Applications
- CRISPR and Genetic Engineering
- Nanocluster Synthesis and Applications
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Nanopore and Nanochannel Transport Studies
- Graphene and Nanomaterials Applications
- bioluminescence and chemiluminescence research
- Cancer, Hypoxia, and Metabolism
- Exercise and Physiological Responses
- Herpesvirus Infections and Treatments
Instituto de Investigación Sanitaria Aragón
2015-2024
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2019-2024
Instituto de Salud Carlos III
2022-2024
Universidad de Zaragoza
2021-2024
Escuela Nacional de Sanidad
2024
Fundacion Agencia Aragonesa para la Investigacion y el Desarrollo
2011-2023
Instituto Aragonés de Ciencias de la Salud
2009-2023
Centro de Investigación Biomédica en Red de Cáncer
2023
Universidad San Jorge
2021
Centro Médico Sanitas Zaragoza
2016-2019
Exosomes are considered key elements for communication between cells, but very little is known about the mechanisms and selectivity of transference processes involving exosomes released from different cells.In this study we have investigated transfer hollow gold nanoparticles (HGNs) cells when these HGNs were loaded within secreted by human placental mesenchymal stem (MSCs). These successfully incorporated in MSCs exosome biogenesis pathway as HGNs-loaded exosomes. Time-lapse microscopy...
Techniques tested for encapsulation of large gold theragnostic nanoparticles in cell-derived exosomes.
Abstract Background Exosomes are endocytic-extracellular vesicles with a diameter around 100 nm that play an essential role on the communication between cells. In fact, they have been proposed as candidates for diagnosis and monitoring of different pathologies (such Parkinson, Alzheimer, diabetes, cardiac damage, infection diseases or cancer). Results this study, magnetic nanoparticles (Fe 3 O 4 NPs) were successfully functionalized exosome-binding antibody (anti-CD9) to mediate capture in...
Heterogeneous catalysis has emerged as a promising alternative for the development of new cancer therapies. In addition, regarding tumor microenvironment reactor with very specific chemical features provided perspective in search catalytic nanoarchitectures action against species playing key role metabolism. One these is glutathione (GSH), whose depletion cornerstone emerging strategies oncology, since this metabolite plays pivotal regulatory antioxidant agent, dampening harmful effects...
Over the last decade, exosomes from diverse biological sources have been proposed as new natural platforms in drug delivery. Translation of these nanometric tools to clinical practice requires deep knowledge their pharmacokinetic properties and biodistribution. The are sometimes evaluated using biochemical histological techniques that considerably invasive. As an alternative, we present radiochemical labeling milk-derived based on reduced 99mTc (IV) without modifying physicochemical...
Deoxyribonucleic acid (DNA) represents the main reservoir of genetic information in cells, which is why it protected nucleus. Entry into nucleus is, general, difficult, as nuclear membrane a selective barrier to molecules longer than 40 kDa. However, some cases, size certain nanoparticles (NPs) allows their internalization nucleus, thus causing direct effect on DNA structure. NPs can also induce indirect effects through reactive oxygen species (ROS) generation. In this context, nanomaterials...
Abstract The lack of safe and efficient systemic gene delivery vectors has largely reduced the potential therapy in clinic. Previously, we have reported that polypropylenimine dendrimer PPIG3/DNA nanoparticles are capable tumor transfection upon administration tumor-bearing mice. To be safely applicable clinic, it is crucial to investigate colloidal stability monitor exact biodistribution transfer whole body live subject. Our biophysical characterization shows dendrimers, when complexed with...
Abstract Background Mesenchymal stem cells (MSCs) have been promoted as an attractive option to use cellular delivery vehicles carry anti-tumor agents, owing their ability home into tumor sites and secrete cytokines. Multiple isolated populations described MSCs, but despite extensive in vitro characterization, little is known about vivo behavior. The aim of this study was investigate the efficacy efficiency different MSC lineages derived from five sources (bone marrow, adipose tissue,...
Copper plays critical roles as a metal active site cofactor and metalloallosteric signal for enzymes involved in cell proliferation metabolism, making it an attractive target cancer therapy. In this study, we investigated the efficacy of polydopamine nanoparticles (PDA NPs), classically applied removal from water, therapeutic strategy depleting intracellular labile copper pools triple-negative breast models through metal-chelating groups present on PDA surface. By using activity-based...
Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity high biocompatibility. However, producing on a large scale is still challenging. One which they could be isolated in quantities milk. Moreover, regeneration well-known property The present work seeks optimize method for isolating bovine human milk,...
The Na/I symporter (hNIS) promotes concentration of iodine in cells. In cancer gene therapy, this transgene has potential as a reporter for molecular imaging viral biodistribution and therapeutic protein promoting (131)I-mediated radiotherapy. Here, we combined the hNIS an oncolytic adenoviruses targeting colorectal cells.We generated adenovirus (AdIP2) encoding capable selective replication carcinoma selectivity virus was verified vitro vivo. Its spread tumors monitored vivo using...
Mesenchymal stem cells (MSCs) not only can be differentiated into different cell types but also have tropism towards injured or inflamed tissues serving as repair cells.
The present work sheds light on a generally overlooked issue in the emerging field of bio-orthogonal catalysis within tumour microenvironments (TMEs): interplay between homogeneous and heterogeneous catalytic processes. In most cases, previous works dealing with nanoparticle-based TME focus effects obtained (
Background In the present study, we investigated ability of polyethylene glycol (PEG) functionalized gold nanoparticles to function as nonviral vectors in transfection different cell lines, comparing them with commercial lipoplexes. Methods Positively-charged were synthesized using polyethylenimine (PEI) a reducing and stabilizer agent its cytotoxicity was reduced by functionalization PEG. We bound three plasmids sizes (4–40 kpb). Vector internalization evaluated confocal electronic...
The main current challenges in oncology are (1) avoiding systemic side effects therapy, and (2) developing alternative treatment strategies for metastatic tumours. Nanomedicine was assumed to provide answers these issues, but delivering enough therapeutic nanoparticles (NPs) tumours still remains a huge challenge nanomaterials-based treatments. Extracellular vesicles (EVs) play key role cell communication processes can be combined with nanomaterials improve their targeting capabilities. In...
Catalytic cancer therapy targets cells by exploiting the specific characteristics of tumor microenvironment (TME). TME-based catalytic strategies rely on use molecules already present in TME. Amino groups seem to be a suitable target, given abundance proteins and peptides biological environments. Here we show that CuFe2O4 nanoparticles are able foster transaminations with different amino acids pyruvate, another key molecule We observed significant cellulo decrease glutamine alanine levels up...