A5018875160- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Liver Diseases and Immunity
- T-cell and B-cell Immunology
- Renin-Angiotensin System Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
- Cancer Research and Treatments
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Xenotransplantation and immune response
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- Phagocytosis and Immune Regulation
- Pancreatic function and diabetes
- Renal Transplantation Outcomes and Treatments
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Hormonal Regulation and Hypertension
- Endoplasmic Reticulum Stress and Disease
- Systemic Lupus Erythematosus Research
Inserm
2015-2025
Center for Research in Transplantation and Translational Immunology
2020-2025
Institut de Transplantation Urologie en Nephrologie
2013-2025
Nantes Université
2014-2024
Université Paris-Saclay
2021
Centre d'Investigation Clinique de Nantes
2012-2020
Centre Hospitalier Universitaire de Nantes
2009-2020
Transgene (France)
2020
Biogen (Switzerland)
2020
Institut National de Recherche en Santé Publique
2015
T cell exclusion causes resistance to cancer immunotherapies via immune checkpoint blockade (ICB). Myeloid cells contribute by expressing signal regulatory protein-α (SIRPα), an inhibitory membrane receptor that interacts with ubiquitous CD47 control macrophage phagocytosis in the tumor microenvironment. Although CD47/SIRPα-targeting drugs have been assessed preclinical models, therapeutic benefit of selectively blocking SIRPα, and not SIRPγ/CD47, humans remains unknown. We report a potent...
The capacity of the angiotensin II (AngII) agonist [Sar1]AngII, antagonist [Sar1‐I1e8]AngII and non‐peptidic DuP753 to undergo receptor internalization were studied in Chinese hamster ovary cells expressing rat AngII type 1a or 1b receptors (AT AT ) a mutant (Asn 74 unable couple G‐protein. In this expression system, ligand‐induced are similar. Moreover, peptidic ligands, either antagonist, induce significant 1 receptors, but is far less potent. Finally, normal Asn demonstrates that...
The role of the renin-angiotensin system has been investigated by overexpression or inactivation its different genes in animals. However, there is no data concerning effect constitutive activation any component system. A knockin mouse model constructed with a gain-of-function mutant Ang II receptor, type 1A (AT(1A)), associating constitutively activating mutation (N111S) C-terminal deletion, which impairs receptor internalization and desensitization. In vivo consequences this expression...
Immunotherapy is a promising strategy against hepatocellular carcinoma (HCC). We assessed the therapeutic effects of stimulating CD137, member TNF receptor family, with agonistic monoclonal antibodies (mAb). Agonistic anti-CD137 mAb treatment was tested on two in situ models HCC immunocompetent mice. also studied mediators involved at different time points. In an orthotopic consistently leads to complete tumor regression 40–60% animals. The protection long lasting animals responding...
Cancer immunotherapy aims to harness the immune system combat malignant processes. Transformed cells harbor diverse modifications that lead formation of neoantigens, including aberrantly expressed cell surface carbohydrates. Targeting tumor-associated carbohydrate antigens (TACA) hold great potential for cancer immunotherapy. N-glycolylneuraminic acid (Neu5Gc) is a dietary non-human immunogenic accumulates on human cells, thereby generating neoantigens. In mice, passive with anti-Neu5Gc...
Autoimmune hepatitis (AIH) is a rare disease characterized by an immune attack of the liver. This study consists comprehensive analysis alterations related to AIH at diagnosis, and during remission phase under treatment. A total 37 major lymphocyte populations were analyzed from peripheral blood new‐onset patients (AIHn; n = 14), with controlled (n 11), healthy subjects 14). Liver biopsy analyses performed complete phenotypic analysis. Four significantly altered in AIHn diagnosis compared...
The Na/I symporter (hNIS) promotes concentration of iodine in cells. In cancer gene therapy, this transgene has potential as a reporter for molecular imaging viral biodistribution and therapeutic protein promoting (131)I-mediated radiotherapy. Here, we combined the hNIS an oncolytic adenoviruses targeting colorectal cells.We generated adenovirus (AdIP2) encoding capable selective replication carcinoma selectivity virus was verified vitro vivo. Its spread tumors monitored vivo using...
Granzyme B-expressing B cells have been shown to be an important regulatory cell subset in humans. However, it is unclear which subpopulations of express GZMB under normal conditions and protocols effectively induce ex vivo expansion GZMB+ cells. We found that the peripheral blood individuals, plasmablasts were major subpopulation expressed GZMB. when using vitro plasmablast differentiation protocol, we obtained only 2% Nevertheless, mixture containing IL-21, anti-BCR, CpG...
Abstract Two well‐characterized carbohydrate epitopes are absent in humans but present other mammals. These galactose‐α1,3‐galactose (αGal) and N ‐glycolylneuraminic acid (Neu5Gc) which introduced by the activities of two enzymes including α(1,3) galactosyltransferase (encoded GGTA1 gene) CMP‐Neu5Gc hydroxylase CMAH that inactive cattle. Hence, bovine‐derived products antigenic who receive bioprosthetic heart valves (BHVs) or those suffer from red meat syndrome. Using programmable nucleases,...
Heterologous polyclonal antibodies might represent an alternative to the use of convalescent plasma or monoclonal (mAbs) in coronavirus disease (COVID-19) by targeting multiple antigen epitopes. However, heterologous trigger human natural xenogeneic antibody responses particularly directed against animal-type carbohydrates, mainly N-glycolyl form neuraminic acid (Neu5Gc) and α1,3-galactose, potentially leading serum sickness allergy. Here, we immunized cytidine...
Abstract Autoimmune liver diseases (AILD) involve dysregulated CD4 T cell responses against self-antigens, but how these autoreactive cells relate to tissue pathology remains unclear. Here we perform single-cell transcriptomic and receptor analyses of circulating, self-antigen-specific from patients with AILD identify a subset liver-autoreactive distinct B-helper transcriptional profile characterized by PD-1, TIGIT HLA-DR expression. These share clonal relationships expanded intrahepatic...
To identify the role(s) of third intracellular loop angiotensin II (AngII) type 1A (AT<sub>1A</sub>) receptor in G protein coupling specificity and activation, several chimerae were constructed characterized. The cDNA sequence encoding C-terminal segment AT<sub>1A</sub> (residues 234–240) was replaced with homologous regions α<sub>1B</sub> adrenergic (α<sub>1B</sub>-AR), β<sub>2</sub> (β<sub>2</sub>-AR), AngII 2 (AT<sub>2</sub>) receptors. These chimeric receptors stably expressed Chinese...
Xenocell therapy from neonate or adult pig pancreatic islets is one of the most promising alternatives to allograft in type 1 diabetes for addressing organ shortage. In humans, however, natural and elicited antibodies specific xenoantigens, α-(1,3)-galactose (GAL) N-glycolylneuraminic acid (Neu5Gc), are likely significantly contribute xenoislet rejection. We obtained double-knockout (DKO) pigs lacking GAL Neu5Gc. Because Neu5Gc−/− mice exhibit glycemic dysregulations β-cell dysfunctions, we...
Donor-specific anti-HLA antibodies (DSAs) are a major risk factor associated with renal allograft outcomes. As trigger of B cell antibody production, T follicular helper cells (Tfhs) promote DSA appearance. Herein, we evaluated whether circulating Tfhs (cTfhs) the genesis antibody-mediated rejection. We measured cTfh levels on day transplantation and one year after in blood from prospective cohort 237 patients without during first post-transplantation. Total cTfhs were characterized as...
Intramuscular (i.m.) DNA vaccination induces strong cellular immune responses in the mouse, but only at doses that cannot be achieved humans. Because antigen expression is weak after naked injection, we screened five nonionic block copolymers of poly(ethyleneoxide)-poly(propyleneoxide) (PEO-PPO) for their ability to enhance using a beta-galactosidase (betaGal) encoding plasmid, pCMV-betaGal, as immunogen. At high dose, formulation with tetrafunctional 304 (molecular weight [MW] 1,650) and...
Abstract CD4+CD25high regulatory T cells (Treg) protect the host from autoimmune diseases but are also obstacles against cancer therapies. An ideal vaccine would stimulate specific cytotoxic responses and reduce/suppress Treg function. In this study, we showed that Escherichia coli expressing listeriolysin O OVA (E. LLO/OVA) demonstrated remarkable levels of protection OVA-expressing tumor cells. By contrast, E. only OVA) poor protection. High-avidity OVA-specific CTL were induced in...
In a previous study, we identified TRIB1, serine-threonine kinase-like molecule, as biomarker of chronic antibody-mediated rejection human kidneys when measured in peripheral blood mononuclear cells. Here, focused our analysis on specific subset cells that play dominant role regulating immune responses health and disease, so-called CD4(+)CD25(+)Foxp3(+) regulatory T (Tregs). We isolated both murine Treg non-Treg counterparts analyzed TRIB1 Foxp3 mRNA expression by quantitative PCR the...