Abstract Background The inflammation and regeneration process may be accompanied by the shift in M1/M2 polarization of macrophages to adapt extracellular signals. How responded altered immunological environment periodontal niche after stem cell transplantation has never been explored. purpose present study is investigate whether participated tissue homeostasis wound healing during ligament (PDLSC)-based regeneration. Methods A rat defect model was utilized observe PDLSC...

Abstract Loss of periodontal ligament fibroblasts (PDLFs) is one critical issue for regenerating lost tissues. A wide variety regulated cell death pathways, such as apoptosis, pyroptosis, and necroptosis have been proposed in the periodontitis development. The aim present study was to explore whether long-term periodontitis-level butyrate may trigger ferroptosis, a newly characterized iron-dependent PDLFs. Here, we showed that treatment butyrate, an important short-chain fatty acid pocket,...

Stem cell-based therapies have shown great promise in regenerative medicine and continue to generate wide interest future clinical applications. However, the issue of storage preservation stem cells, for applications, still requires extensive investigation.The purpose this study was evaluate effect cryopreservation on capacity bone marrow cells periodontal defects dogs.Bone mesenchymal (BMSCs) were obtained from 5 beagle dogs. After 1 month, cell viability, surface adherence ability,...

Objective This retrospective study aimed to analyze the clinical efficacy of two regenerative surgical methods — Bio-Oss granules combined with barrier membranes and Collagen alone help clinicians achieve better periodontal regeneration outcomes in specific condition. Methodology Patients who underwent surgery from January 2018 April 2022 were retrospectively screened, their radiographic at 6 months postoperatively analyzed. The probing depth (PD), attachment level (CAL), bleeding on (BOP),...

Abstract Periodontal homeostasis is maintained by the dynamic equilibrium between cell death, differentiation and proliferation of resident cells in periodontal microenvironment. Loss ligament fibroblasts (PDLFs) has been a major challenge treatment. This study aimed to investigate exact role necroptotic death diseases. Elevated levels receptor-interacting protein serine-threonine kinases -1 (RIPK1), phosphorylated RIPK3, mixed lineage kinase domain-like (MLKL), MLKL FLIP L were observed...

To eliminate invading pathogens and keep homeostasis, host employs multiple approaches such as the non-inflammation associated-apoptosis, inflammation associated-necroptosis pyroptosis, etc. Necroptosis is known a highly pro-inflammatory form of cell death due to release massive damage-associated molecular patterns (DAMPs). For first time, we reported that Porphyromonas gingivalis induced cellular necroptosis through receptor-interacting protein 1 (RIP1)/RIP3/mixed lineage kinase domain-like...

Human β-defensin 3 (hBD3) is a cationic peptide with immunomodulatory effects on both innate and acquired immune responses. Periodontitis, an inflammatory disease that extends deep into periodontal tissues, causes the loss of supporting structures around tooth. The present study assessed hBD3 as monotherapy for periodontitis in mice explored its potential mechanism. In vivo, inhibited levels tumour necrosis factor (TNF)-α, interleukin-6, matrix metalloprotease-9 periodontium exposed to...

A finely-tuned innate immune response plays a pivotal role in protecting host against bacterial invasion during periodontal disease progression. Hyperlipidemia has been suggested to exacerbate health condition. However, the underlying mechanism not addressed. In present study, we investigated effect of hyperlipidemia on responses pathogen Porphyromonas gingivalis infection. Apolipoprotein E-deficient and wild-type mice at age 20 weeks were used for study. Peritoneal macrophages isolated...

Metabolic reprogramming from oxidative phosphorylation to glycolysis have been implicated in the pathogenesis of inflammatory diseases, such as pulmonary hypertension, rheumatoid arthritis and sepsis. Whether metabolic participates progression bacteriogenic periodontitis has never reported. In present study, we explored changes periodontal ligament cells (PDLSCs) response Porphyromonas gingivalis. (P. gingivalis)-infected PDLSCs showed distinct metabolomics with glycolysis. addition,...

Iron homeostasis plays a crucial role in the combat against pathogen invasion. Ferrous iron can trigger generous production of reactive oxygen species (ROS) by Fenton reaction. Nuclear receptor coactivator 4 (NCOA4), selective cargo to deliver ferritin lysosome, may release ferritin-bound into cytosol. The aim present study was explore whether NCOA4-mediated ferritinophagy participated pathogenesis periodontitis, and its promoting periodontal inflammation.Inflamed healthy tissues were...

Abstract Objective Mitochondria are strained by microbial stimuli in the periodontal niche. Damaged mitochondria cleared mitophagy. The purpose of study was to explore whether mitophagy participated progress periodontitis and activation can inhibit inflammatory responses bacterial infection macrophages. Methods Mitophagy‐related genes were measured healthy inflamed human gingiva. Bone marrow‐derived macrophages (BMDMs) infected with Porphyromonas gingivalis . Dexmedetomidine, urolithin A,...

Most mesenchymal stem cells reside in a niche of low oxygen tension. Iron-chelating agents such as CoCl2 and deferoxamine have been utilized to mimic hypoxia promote cell growth. The purpose the present study was explore whether supplement succinate, natural metabolite tricarboxylic acid (TCA) cycle, can condition human periodontal ligament (hPDLCs). Culturing hPDLCs promoted proliferation, migration, osteogenic differentiation; moreover, shifted metabolism from oxidative phosphorylation...

Abstract Background Periodontal health is of great concern for periodontists and orthodontists in the inter-disciplinary management patients with bimaxillary protrusion. The aim present study to investigate changes alveolar bone maxillary incisor region explore its relationship displacement root apex as well inclination incisors during retraction. Methods Samples this retrospective consisted 38 Cone-beam computed tomography (CBCT) images was taken before(T0) after (T1) treatment. Alveolar...

The purpose of this study was to compare the difference alveolar bone remodeling between adolescents and adults in maxillary incisor area during retraction. This retrospective included 72 female patients who needed moderate anchorage correct bimaxillary protrusion. Subjects were further divided into minor group (n = 36, 11-16 years old) adult 18-35 old). Digital lateral cephalography cone beam CT scanning taken each patient before (T0) after treatment (T1). Cephalometry conducted assess...

Protracting lower second molars and uprighting horizontally impacted third is a significant orthodontic challenge in patients who require the extraction of severely decayed first molars.Here, we describe use biomechanics to upright 90 • -tilted following molar protraction.Herein, introduce technique for by (1) placement superelastic nickel titanium archwires, (2) bonding, (3) repositioning buccal tube tilted position compensate efficiency Ni-Ti (nickel-titanium) wire.The treatment mechanics...

Abstract To investigate and compare the facial asymmetry (hard soft tissues) among skeletal Class I, II, III patients. A total of 221 subjects, including I (n = 80), II 75), 66), were included in study. CBCT, 22 landmarks, 10 tissue landmarks used for measurements index was calculated to assess asymmetry. Statistical analyses one-way ANOVA, Kruskal–Wallis test, Spearman correlation analysis. The patients presented greater than hard 3 (p < 0.05). High found between four their corresponding...

<title>Abstract</title> Background To explore the incidence and risk factors of open gingival embrasures (OGEs) in front region adult none-extraction cases with clear aligner treatment (CAT). Methods Eighty-two patients non-extraction CAT were included this retrospective study. A total 820 tooth sites assessed for OGEs intraoral photographs. These categorized into nonoccurrence group occurrence group. The parameters – including crown shape, root angulation, distance between cementoenamel...

Peri-implantitis is a pathologic condition associated with dental plaque that occurs in the implant tissue and characterized by inflammation of mucous membrane surrounding implant, followed progressive loss supporting bone. In this study, case guided bone regeneration therapy based on control peri-implant was reported. Four years after surgery for left second premolar patient presented "left lower posterior tooth swelling discomfort more than 2 years". The X-ray periapical film showed...

ABSTRACT Objective This case report offers information on accurate diagnostic and soft‐ hard‐tissue reconstruction periodontal surgery deal with “black triangles” in the aesthetic zone of anterior teeth caused by submucous alveolar cleft, which is rare easily overlooked. Clinical Considerations Vertical horizontal bone augmentation was conducted via guided tissue regeneration grafting (ABG) at cleft site where favorable conditions were absent. A customized, vascularized interpositional...

Insufficient vertical space is often a great challenge for prosthodontic treatment of patients with teeth overeruption. This case report describes simple technique using clear aligners to intrude over-erupted molars. A 27-year-old woman was diagnosed left mandibular dentition defect, yet the third molar presented in lower arch. However, prosthetic rehabilitation limited by opposing Clear therapy (CAT) used regain necessary interocclusal space. After initial distalization, subsequent 2.14 mm...

ABSTRACT Objectives To evaluate the accuracy of Invisalign ClinCheck in predicting open gingival embrasures (OGEs) and to identify predictors OGEs adult extraction cases. Materials Methods Fifty-seven patients treated with four first premolar extractions were included this retrospective study. measured maxillary mandibular anterior regions using posttreatment intraoral photographs (actual OGEs) final step from treatment plan (predicted OGEs). Prediction performance indicators including...