A5057163074- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Liver Disease and Transplantation
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
- Heme Oxygenase-1 and Carbon Monoxide
- Diabetes and associated disorders
- Neonatal Health and Biochemistry
- Trace Elements in Health
- HIV Research and Treatment
- Alcohol Consumption and Health Effects
- Influenza Virus Research Studies
- Chronic Lymphocytic Leukemia Research
- Hormonal and reproductive studies
- Immunodeficiency and Autoimmune Disorders
- Organ Transplantation Techniques and Outcomes
- Animal Genetics and Reproduction
- HIV/AIDS Research and Interventions
- Glycosylation and Glycoproteins Research
- Skin and Cellular Biology Research
- Systemic Lupus Erythematosus Research
- Telomeres, Telomerase, and Senescence
- Carcinogens and Genotoxicity Assessment
- Effects and risks of endocrine disrupting chemicals
University of Iowa
2008-2021
Veterans Health Administration
2007-2021
University of Lübeck
2019
University Hospital Schleswig-Holstein
2019
Iowa City VA Health Care System
2019
Iowa City VA Medical Center
1995-2016
Praxis
2008-2011
United States Department of Veterans Affairs
1999-2011
Endokrinologikum
2009-2011
University of Iowa Hospitals and Clinics
1998-2010
ABSTRACT Hepatitis C virus (HCV) or HCV–low-density lipoprotein (LDL) complexes interact with the LDL receptor (LDLr) and HCV envelope glycoprotein E2 interacts CD81 in vitro. However, interactions LDLr have not been clearly described. Using sucrose gradient-purified low-density particles (1.03 to 1.07 g/cm 3 ), intermediate-density (1.12 1.18 recombinant protein, control proteins, we assessed binding MOLT-4 cells, foreskin fibroblasts, LDLr-deficient fibroblasts at 4°C by flow cytometry...
Background. Chronic hepatitis C virus (HCV)–induced liver fibrosis involves upregulation of transforming growth factor (TGF)–β and subsequent hepatic stellate cell (HSC) activation. MicroRNAs (miRNAs) regulate HCV infection HSC Methods. TaqMan miRNA profiling identified 12 families differentially expressed between chronically HCV-infected human livers uninfected controls. To identify pathways affected by miRNAs, we developed a new algorithm (pathway analysis conserved targets), based on the...
Many veterans may not be candidates for hepatitis C virus (HCV) treatment due to contraindications therapy. The aims of this study were determine the proportion HCV-infected who eligible interferon alfa and ribavirin therapy evaluate barriers HCV treatment.We prospectively enrolled 4,084 referred over a 1-yr period at 24 Veterans Affairs (VA) Medical Centers. Treatment candidacy was assessed using standardized criteria opinion treating clinician.Overall, 32.2% (95% CI, 30.8-33.7%) according...
Abstract Oxidative injury to hepatocytes occurs as a result of hepatitis C virus (HCV) infection and replication. Modulation host cell antioxidant enzymes such heme oxygenase-1 (HO-1) may be useful therapeutically minimize cellular injury, reduce viral replication, attenuate liver disease. In this report, we evaluated the effects HO-1 overexpression on HCV replication hepatocellular injury. Full-length (FL) (Con1) or nonstructural (NS) replicons (I 389 NS3-3′) were transfected with complete...
Abstract Chronic hepatitis C virus (HCV) infection leads to increased oxidative stress in the liver. Hepatic antioxidant enzymes provide an important line of defense against injury. To understand responses hepatocytes different HCV proteins, we compared changes antioxidative HCV‐core and HCV‐nonstructural protein expressing hepatocyte cell lines. We found that expression lines as determined by oxidant‐sensitive probe 5‐(and‐6)‐chloromethyl‐2′,7′‐dichlorodihydrofluorescein diacetate (CM‐DCFH...
Induction of heme oxygenase-1 (HO-1) inhibits hepatitis C virus (HCV) replication. Of the products reaction catalyzed by HO-1, iron has been shown to inhibit HCV ribonucleic acid (RNA) polymerase, but little is known about antiviral activity biliverdin (BV). Herein, we report that BV viral replication and protein expression in a dose-dependent manner replicons cells harboring infectious J6/JFH construct. Using SensoLyte 620 Protease Assay with wide wavelength excitation/emission (591 nm/622...
Summary Sustained virologic response ( SVR ) is the standard measure for evaluating to therapy in patients with chronic hepatitis C CHC ). The aim of this study was prospectively assess durability pivotal studies peginterferon PEG ‐ IFN α‐2b or α‐2b. We conducted two phase 3b long‐term follow‐up previously treated eight prospective randomized and/or Patients who achieved [undetectable virus HCV RNA 24 weeks after completion treatment] were eligible inclusion these studies. In total, 636...
The in vivo cross-linking of proteins to DNA intact Novikoff ascites hepatoma cells exposed the chromium salt K2Cr04 was studied.DNA-protein complexes were assayed by high speed centrifugation solubilized buffered 4% sodium dodecyl sulfate and electrophoretic identification associated with DNA-containing pellets.Further evidence DNA-protein complexes, not dissociable this buffer, obtained CsCl gradient centrifugation.Time dependence experiments showed that detectable occurred after for at...
In hepatitis C virus (HCV)-positive liver transplant recipients, infection of the allograft and recurrent disease are important problems. Increased donor age has emerged as an variable affecting patient graft survival; however, specific cutoffs risk ratios for poor histologic outcomes survival not clear.A longitudinal database all HCV-positive patients transplanted at our center during 11-year period was used to identify 111 who received 124 transplants. Graft histological endpoints (severe...
Cross-linking of proteins to DNA in live, intact Novikoff ascites hepatoma cells exposed vitro different concentrations CuS04, Pb(N03)2, HgClz, and A1C13 was studied.Protein-DNA complexes were separated by high-speed centrifugation solubilized buffered 4% sodium dodecyl sulfate assayed electrophoretic separation associated with the DNA-containing pellets.Concentration dependence experiments showed that optimal cross-linking occurred at metal 0.5 m M for HgCIZ, AlC13 while Ph(N03)2 5 mM.For...
ABSTRACT GB virus C (GBV-C or hepatitis G virus) is a recently described flavivirus which frequently leads to chronic viremia in humans. Although GBV-C associated with acute posttransfusion hepatitis, it not clear if the pathogenic for We constructed full-length cDNA from plasma of person viremia. Peripheral blood mononuclear cells (PBMCs) transfected RNA transcripts this clone resulted viral replication. This was demonstrated by serial passage cell culture supernatants, detection increasing...
Summary: Hepatitis C virus (HCV) viremia may occur in persons without detectable HCV antibodies and has been reported as many 5.5% of HIV-positive persons. To better characterize serosilent infection, the authors prospectively tested 131 102 HIV-negative control subjects with diabetes for presence antibody (Ab) RNA. Thirty 31 Ab-positive (AbP) people positive RNA did both AbP, subjects. Similarly, none 100 HIV-negative, Ab-negative (AbN) was (p < .001). In contrast, 19 HIV-positive, AbN met...
Antioxidant enzymes, including heme oxygenase (HO)-1, are an important line of defense against oxidant-mediated liver injury. Because hepatitis C virus (HCV) infection appears to increase the production oxidants, we evaluated levels antioxidant enzymes and HO-1 in liver-biopsy samples from HCV-infected patients by immunoblot semiquantitative reverse-transcriptase polymerase chain reaction. In samples, immunoreactive mRNA were >4-fold lower than control but superoxide dismutase catalase...
ABSTRACT Hepatitis G virus (HGV or GB-C virus) is a newly described that closely related to hepatitis C (HCV). Based on sequence analysis and by evaluation of translational initiation codon preferences utilized during in vitro translation, HGV appears have truncated absent core protein at the amino terminus polyprotein. Consequently, biophysical properties may be very different from those HCV. To characterize particle types, we evaluated plasma chronically infected individuals with without...
Reverse transcription-polymerase chain reaction was used to identify hepatitis C virus (HCV) RNA in peripheral whole blood (WB) and plasma samples from 15 patients with chronic, unexplained hepatitis. These were serologically negative for A, B, classified as having chronic non-A, non-B, non-C (NANBNC). HCV repeatedly detected WB 10 (67%). In contrast, only 5 intermittently positive. Statistically, detection significantly more sensitive than plasma. Nucleic acid hybridization genotypic...
Previous experiments using a cationic surfactant to detect hepatitis C virus (HCV) RNA in whole blood (WB) suggested that WB was more plentiful source of viral than plasma. The relative HCV titers WB, plasma, peripheral mononuclear cells (PBMC), neutrophils, and red (RBC)/platelets from 10 patients with chronic infection were compared. contained significantly which PBMC, or RBC/platelets (P < .001). To determine if this increased sensitivity clinically relevant, results plasma assays...
High concentrations of androgens in itro [10-6 and 10-7M 5α-dihydrotestosterone (DHT) testosterone (T)] translocate the estrogen receptor from cytoplasmic to nuclear fraction immature rat uterus, androgen translocated sites are capable eliciting synthesis specific uterine “induced protein” (IP), formerly attributed estrogenic compounds only. The magnitude translocation IP induction is related concentration androgen, and, at equal concentrations, DHT more effective than T. Competitive...
Hepatitis C virus, human immunodeficiency and hepatitis B virus are chronic viral infections that cause considerable morbidity mortality throughout the world. In decades following identification sequencing of these viruses, in vitro experiments demonstrated heme oxygenase-1, its oxidative products, related compounds oxygenase system virucidal for all three viruses. The purpose this review is to critically evaluate summarize seminal studies described characterized remarkable behavior. It will...