A5003682421- Hepatitis C virus research
- Antioxidant Activity and Oxidative Stress
- Eicosanoids and Hypertension Pharmacology
- Liver Disease and Transplantation
- Transplantation: Methods and Outcomes
- Cerebrovascular and Carotid Artery Diseases
- Free Radicals and Antioxidants
- Cardiovascular Health and Disease Prevention
- Medical Image Segmentation Techniques
- Renal Transplantation Outcomes and Treatments
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Viral Infections and Immunology Research
- Inflammatory mediators and NSAID effects
- Radiomics and Machine Learning in Medical Imaging
- HIV/AIDS drug development and treatment
- Selenium in Biological Systems
- Pharmacogenetics and Drug Metabolism
- Drug-Induced Hepatotoxicity and Protection
- Paraquat toxicity studies and treatments
- Meta-analysis and systematic reviews
- Peroxisome Proliferator-Activated Receptors
- Respiratory Support and Mechanisms
- Medical Imaging Techniques and Applications
- Advanced Radiotherapy Techniques
Vanderbilt University Medical Center
1994-2024
Vanderbilt University
1992-2024
VA Tennessee Valley Healthcare System
2003-2024
Centre for Imaging Technology Commercialization
2019
Western University
2011-2013
Robarts Clinical Trials
2010-2012
Nashville Oncology Associates
2005
University of Waterloo
2004
National Institute of Environmental Health Sciences
1998
National Institutes of Health
1998
We recently reported the discovery of a series bioactive prostaglandin F2-like compounds (F2-isoprostanes) that are produced in vivo by free radical-catalyzed peroxidation arachidonic acid independent cyclooxygenase enzyme. Inasmuch as phospholipids readily undergo peroxidation, we examined possibility F2-isoprostanes may be formed situ on phospholipids. Initial support for this hypothesis was obtained finding levels measured after hydrolysis lipids extracted from livers rats treated with...
Growth factors and tumor promoters have been shown to play a role in intestinal epithelial growth regulation transformation. In this study, transforming factor-alpha (TGF alpha) the promoter, tetradecanoyl phorbol acetate (TPA), are stimulate production of eicosanoids by rat (RIE-1) cells culture. A 4.5-kb mRNA, which hybridizes mouse cyclooxygenase-2 cDNA probe, is elevated 18-fold within 30 min after TGF alpha or TPA treatment. Stimulation RIE-1 with leads increase protein (M(r)...
These studies examine the in vivo formation of a unique series PGF2-like compounds (F2-isoprostanes) derived from free radical-catalyzed nonenzymatic peroxidation arachidonic acid. We have previously shown that levels these increase up to 50-fold rats administered CCl4. To understand further vivo, we carried out experiments assessing factors influencing their generation. After CCl4 (2 ml/kg) was rats, plasma F2-isoprostanes increased 55-fold by 4 h. Levels declined thereafter, but at 24 h,...
For patients awaiting heart transplant, hepatitis C-positive donors offer an opportunity to expand the donor pool, shorten wait times, and decrease wait-list mortality. While early reported outcomes among few transplant recipients have been promising, knowledge of 1-year in larger cohorts is critical shared decision-making with about this option.To better define association volumes, duration, transmission cure donor-derived C, morbidity mortality at 1 year.This was a prospective,...
A dose of diquat below the amount injurious to selenium-replete animals causes lipid peroxidation and massive liver necrosis in selenium-deficient rats. The current study was undertaken characterize with respect injury correlate presence several selenoproteins protective effect selenium. Lipid assessed by measurement F2 isoprostanes. Diquat caused an increase plasma isoprotanes. gradient these compounds detected across some animals, indicating that this organ a source time-course experiment...
Abstract Objective . Free radical–induced oxidative stress with consequent lipid peroxidation and resultant tissue damage has been suggested as a potential mechanism of the pathogenesis scleroderma. However, because reliable measurement in vivo is difficult, it not possible to adequately examine this hypothesis. We have previously described series bioactive prostaglandin F 2 –like compounds, termed ‐isoprostanes, produced humans by noncyclooxygenase, free radical–catalyzed, arachidonic acid...
Free radicals are thought to play an important role in many types of tissue injury. Recently, we reported that a series prostaglandin F2-like compounds (F2-isoprostanes) capable exerting potent biological activity produced vivo by free radical-induced lipid peroxidation. Their formation is independent the cyclooxygenase enzyme and has been shown increase profoundly animal models radical injury We now report identification F-ring isoprostane metabolites human urine plasma utilizing gas...
Many veterans may not be candidates for hepatitis C virus (HCV) treatment due to contraindications therapy. The aims of this study were determine the proportion HCV-infected who eligible interferon alfa and ribavirin therapy evaluate barriers HCV treatment.We prospectively enrolled 4,084 referred over a 1-yr period at 24 Veterans Affairs (VA) Medical Centers. Treatment candidacy was assessed using standardized criteria opinion treating clinician.Overall, 32.2% (95% CI, 30.8-33.7%) according...
Growth factors have been shown to play a role in intestinal epithelial growth regulation and transformation. Utilizing standard differential cloning techniques, we isolated factor-inducible gene (RS-2) from rat cells that has approximately 95% homology the mouse mitogen-inducible cyclooxygenase (COX-2) at amino acid level. This cDNA hybridizes 4.5-kb mRNA transforming factor (TGF)-alpha-stimulated (RIE-1) is constitutively expressed vivo adult kidney brain. Nuclear run-on experiments...
The isoprostanes (IsoPs) are novel bioactive prostaglandin-like compounds produced in vivo by free radical-catalyzed peroxidation of arachidonyl-containing lipids. Previously, we have identified IsoPs containing F-type and D- E-type prostane rings that formed reduction rearrangement IsoP endoperoxide intermediates, respectively. We now explore whether thromboxane B2 (TxB2)-like compounds, termed B2-isothromboxanes (B2-IsoTxs), endoperoxides. Detection these was carried out using a stable...
This study was designed to determine whether transforming growth factor alpha (TGF alpha) protects rat gastric mucosa against ethanol- and aspirin-induced injury. Systemic administration of TGF dose-dependently decreased 100% ethanol-induced mucosal injury; a dose 50 micrograms/kg delivered intraperitoneally 15 min before ethanol macroscopic injury by > 90%. At the microscopic level, prevented deep necrotic lesions reduced disruption surface epithelium. Pretreatment with orogastric (200...
Purpose: Three-dimensional ultrasound (3D US) of the carotid artery provides measurements arterial wall and plaque [vessel volume (VWV)] that are complementary to one-dimensional measurement intima-media thickness. 3D US VWV requires an observer delineate media–adventitia boundary (MAB) lumen–intima (LIB) artery. The main purpose this work was develop evaluate a semiautomated segmentation algorithm for delineating MAB LIB from images. Methods: To segment LIB, authors used level set method...
The interaction of forskolin with adenylate cyclase was studied by evaluating its effect on metal and metal-ATP kinetics measuring protective when the enzyme subjected to denaturation conditions.The solubilized calmodulin-and forskolin-sensitive from brain particulate platelets were inactivated upon preincubation N-ethylmaleimide.Forskolin protected against this inactivation in a concentration-dependent manner demonstrated Kd values 6.3 7.6 PM for platelet cyclases, respectively.Protection...
Purpose: To quantitatively evaluate local carotid arterial statin effects in 3D US images using multiclassifier image texture analysis tools. Methods: Texture tools were used to the effect of 80 mg atorvastatin administered daily patients with stenosis compared those treated placebo. Using three-dimensional ultrasound images, 270 features from seven techniques extracted manually segmented arteries based on intima-media boundary [vessel wall (VW)]. Individual previously determined changes VW...
Purpose: Three‐dimensional ultrasound (3DUS) vessel wall volume (VWV) provides a 3D measurement of carotid artery remodeling and atherosclerotic plaque is sensitive to temporal changes burden. Unfortunately, although 3DUS VWV many advantages compared measurements arterial thickening or alone, it still not widely used in research clinical practice because the inordinate amount time required train observers generate measurements. In this regard, semiautomated methods for segmentation...
Bromfenac sodium (Duract) is a phenylacetic acid-derived nonsteroidal anti-inflammatory agent introduced in the United States 1997 and withdrawn 1998. We describe first case of fulminant hepatic failure associated with this treated successfully liver transplantation. Similarities to hepatotoxicity related agents discussed.
The dipyridyl herbicide diquat undergoes redox cycling in vivo resulting superoxide generation. Diquat administration causes hepatic and renal toxicity rodents. Selenium deficiency worsens this injury lipid peroxidation is a prominent feature of the toxicity. However, there limited data regarding role diquat-induced selenium-adequate animals. In addition, little known about effect glutathione-modulating agents on vivo. F2-isoprostanes are novel prostanoids which, both free plasma esterified...