A5103268936- Hormonal Regulation and Hypertension
- Vitamin D Research Studies
- Tea Polyphenols and Effects
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Cancer Mechanisms and Therapy
- Urinary and Genital Oncology Studies
- Advanced Breast Cancer Therapies
- Bladder and Urothelial Cancer Treatments
- Bone Tumor Diagnosis and Treatments
- Musculoskeletal synovial abnormalities and treatments
- Heavy Metals in Plants
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Biochemical Analysis and Sensing Techniques
- Immunotherapy and Immune Responses
- Medical Imaging and Analysis
- Cancer, Hypoxia, and Metabolism
- Sarcoma Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Adrenal Hormones and Disorders
- Mechanisms of cancer metastasis
Southwest University
2024
Tea Research Institute
2024
Xiangya Hospital Central South University
2020
Central South University
2020
Roswell Park Comprehensive Cancer Center
2010-2019
South China Agricultural University
2014-2017
Qi2
2003
Calcitriol, a regulator of calcium homeostasis with antitumor properties, is degraded by the product CYP24A1 gene, which downregulated in human prostate cancer unknown mechanisms. We found that expression inversely correlated promoter DNA methylation cell lines. Treatment methyltransferase inhibitor 5-aza-2'-deoxycytidine (DAC) activates cells. In vitro represses its activity. Furthermore, inhibition histone deacetylases trichostatin A (TSA) enhances Quantitative chromatin...
MMP13 (matrix metallopeptidase 13) plays a key role in bone metabolism and cancer development, but has no known functions Alzheimer's disease. In this study, we used high-throughput small molecule screening SH-SY5Y cells that stably expressed luciferase reporter gene driven by the BACE1 (β-site amyloid precursor protein cleaving enzyme 1) promoter, which included portion of 5' untranslated region (5'UTR). We identified CL82198, selective inhibitor MMP13, decreased levels cultured neuronal...
Epidemiological data suggest an important role of vitamin D signaling in cancer development and progression, experimental studies demonstrate that the active metabolite 1α, 25-dihydroxyvitamin D₃ (1,25D₃) has broad spectrum antitumor activity. Hypercalcemia often been suggested to limit clinical application these data. The 14-epi-analog 1,25D₃, inecalcitol [19-nor-14-epi-23-yne-1,25-(OH)₂D₃; TX522], was developed have superagonistic activities but low hypercalcemia potential. We examined...
(-)-Epigallocatechin gallate (EGCG) and theacrine are involved in imparting tea with its astringent bitter tastes. This study investigated the effect of on astringency EGCG molecular mechanism. Sensory evaluation was used to intensities solutions presence absence various concentrations theacrine. The results indicated a considerable increase values EGCG-theacrine compared those alone. Furthermore, dynamic light scattering (DLS) dynamics simulations (MD) were explore interaction mechanisms....
Vitamin D has broad range of physiological functions and antitumor effects. 24-Hydroxylase, encoded by the CYP24A1 gene, is key enzyme for degrading many forms vitamin including most active form, 1,25D(3). Inhibition enhances 1,25D(3) activity. To isolate regulators expression in prostate cancer cells, we established a stable cell line PC3 with promoter driving luciferase to screen small molecular library compounds that inhibit From this screening, identified, 4,5,6,7-tetrabromobenzimidazole...
Tea nano-aggregates spontaneously assembled in clear tea infusions are considered as the precursors of cream, although their molecular basis remains obscure. Here, we characterized green from Camellia ptilophylla, a peculiar variety with 6.0% theobromine, and sinensis control for comparative purpose. Numerous negatively charged spherical colloidal particles 50-100 nm diameter were primarily found both infusions. Catechins, proteins, carbohydrates confirmed dominant components...
In our previous study, phloridzin, sucrose, l-alanine, and dulcitol presented synergistic effects in Camellia nanchuanica black tea (NCBT). This study aims to verify the of aforementioned sweet taste compounds mechanism involved. By conducting σ–τ plot analysis, phloridzin at recognition threshold concentration (phl) exhibited with different concentrations sucrose (Lsuc–6suc). Various their combinations were selected investigate impact on receptor cells. The results revealed that...
Abstract Human high molecular weight-melanoma associated Ag (HMW-MAA) mimics have been shown to elicit HMW-MAA-specific humoral immune responses that appear be clinically beneficial. This finding has stimulated interest in characterizing the mechanism(s) underlying ability of elicited Abs exert an anti-tumor effect. To address this question, present study, we generated by sequentially immunizing rabbits with peptide P763.74, which HMW-MAA determinant recognized mAb 763.74, and HMW-MAA+...
The active metabolite of vitamin D 1α,25-dihydroxycholecalciferol (1,25D(3) ) has exhibited broad-spectrum antitumor activity in xenograft animal models. However, its against metastatic disease not been extensively investigated.Squamous cell carcinoma (SCC) or 1,25D(3) -resistant variant SCC-DR cells were treated with . Actin organization was examined by immunofluorescence assay. Cell migration assessed "wound" healing and chemotactic assays. invasion a Matrigel-based assay situ zymography....
Bladder cancer recurrence following cystectomy remains a significant cause of bladder specific mortality. Residual cells contribute to due tumor spillage or undetectable preexisting micrometastatic clones. We detected and quantified residual in pelvic washing using ultradeep targeted sequencing. compared the levels with clinical variables recurrence.The primary specimen was available 17 patients who underwent robot-assisted radical cystectomy. All tumors had negative surgical margins. Pelvic...
// Wei Luo 1 , Qiang Hu 3 Dan Wang Kristin K. Deeb 2 ,Yingyu Ma Carl D. Morrison Song Liu Candace S. Johnson and Donald L. Trump 4 Department of Pharmacology Therapeutics, Roswell Park Cancer Institute, Buffalo, New York Pathology, Biostatistics Bioinformatics, Medicine, Correspondence: Trump, email: Keywords : Endothelial cells, Gene expression, DNA methylation, prostate cancer Received August 9, 2013 Accepted 21, Published Abstract cells (ECs) are an important component involved in the...
Abstract Background Vitamin D 3 (VitD) deficiency is linked to increased incidence and worse survival in bladder cancer ( BC a). In addition cystectomy, patients are treated with cisplatin‐based chemotherapy, however 30%‐50% of do not benefit from this treatment. The effects VitD on response chemotherapy remain unknown. Methods To test supplementation the cisplatin we analyzed patient serum levels correlated that survival. vivo, deficient mice were cisplatin, or without pretreatment active...
Abstract Peptide mimics isolated from phage display peptide libraries by panning with self-tumor-associated Ag (TAA)-specific mAbs are being evaluated as immunogens to implement active specific immunotherapy. Although TAA-specific mAb commonly used isolate mimics, no information is available regarding the Ab characteristics required immunogenic TAA mimics. To address this question, we have 763.74 and GH786, which recognize same or spatially close antigenic determinant(s) of human high...
Metastasis is the major cause of bladder cancer death. 1,25D
Abstract Epidemiological data suggest an important role of vitamin D signaling in cancer progression, and experimental studies demonstrate that the active metabolite 1α, 25-dihydroxyvitamin D3 (1,25D3) has broad spectrum anti-tumor activity. Hypercalcemia often been suggested to limit clinical application these data. The 14-epi-analog 1,25D3, inecalcitol (19-nor-14-epi-23-yne-1,25-(OH)2D3; TX522), was developed have superagonistic activities but low hypercalcemia potential. We examined...
Abstract Hypoxia-inducible factor (HIF) plays an important role in the tumorigenesis of renal cell carcinoma (RCC). Mutational inactivation von Hippel-Lindau (VHL) tumor suppressor gene, which is most frequent molecular event, has been implicated HIF overexpression. Recent studies found that PIM kinases increase expression cancer. The Kinases are a family highly conserved serine/threonine consisting three isoforms (PIM1, PIM2, and PIM3). play multiple roles promoting proliferation survival...
Abstract Bladder cancer is the 5th most common in United States. 30% of cases are advanced stage requiring multimodality therapy at diagnosis and many which first diagnosed when superficial will progress to more disease. High recurrence progression rate drug resistance highlight need for new therapeutic strategies improve clinical outcomes. 1,25-dihydroxyvitamin D3 (1,25D3), active vitamin D metabolite, has demonstrated broad spectrum antitumor activities vitro vivo. In addition, 1,25D3...
Abstract Dysregulation of vitamin D signaling and metabolism enzymes is a frequent change in many cancers may confound approaches to -D based therapies. The mechanisms by which aberrant lead resistance action are poorly understood. kidney plays critical role synthesis metabolism; renal cell carcinoma (RCC) cells dysregulated. Recently, we discovered that an oncogenic protein kinase PIM-1 involved D-induced expression 24-hydroxylase, key catalytic enzyme encoded CYP24A1 gene, bladder prostate...