A5074807174- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Genetic Neurodegenerative Diseases
- TGF-β signaling in diseases
- Urological Disorders and Treatments
- Veterinary Medicine and Surgery
- 3D Printing in Biomedical Research
- Ovarian function and disorders
- Neuroendocrine regulation and behavior
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
- Bone Metabolism and Diseases
- Effects of Environmental Stressors on Livestock
- Meat and Animal Product Quality
- Animal Genetics and Reproduction
- Endometriosis Research and Treatment
- thermodynamics and calorimetric analyses
- Urban Development and Cultural Heritage
- Physiological and biochemical adaptations
- Animal Nutrition and Health
- Selenium in Biological Systems
- Organ and Tissue Transplantation Research
- Birth, Development, and Health
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2018-2024
Duke University
2024
Duke University Hospital
2024
Duke Medical Center
2024
Philips (United Kingdom)
2022
Polish Academy of Sciences
2020
University of Life Sciences in Poznań
2010-2017
Poznan University of Medical Sciences
2013
University of Life Sciences in Lublin
2009
Abstract Polyglutamine (polyQ) diseases are incurable neurological disorders caused by CAG repeat expansion in the open reading frames (ORFs) of specific genes. This type mutation HTT gene is responsible for Huntington’s disease (HD). repeat-targeting artificial miRNAs (art-miRNAs) were shown as attractive therapeutic approach polyQ they allele-selective decrease level mutant proteins. Here, using models, we aimed to demonstrate how miRNA-based expression regulation dependent on target...
Abstract Background Cystic endometrial hyperplasia-pyometra complex (CEH-P) is one of the most common uteropathies in bitches. In diseases with mild or obscure clinical signs and normal uterine size, a diagnosis based on assessment might be incorrect. The main aim research was to determine morphological variables accompanying bitches microscopic evaluation. Consequently, obtained results can used create new classification system for pathological changes during development CEH-P, diagnosed by...
The TGFB superfamily genes are involved in several important cell functions, including proliferation and differentiation, the role of expression these growth development theca granulosa cells is well recognised. However, dependence between stage oocyte maturation or follicular size pigs still not entirely known. This study was aimed at investigating pattern GDF9, TGFB1, TGFB2 TGFB3 porcine oocytes before after vitro (IVM) as collected from follicles different sizes. RQ-PCR performed to...
The most common uterine diseases affecting bitches are cystic endometrial hyperplasia (CEH) and pyometra. neuropeptide phoenixin (PNX) its receptor (GPR173) potential key factors involved in the proliferative inflammatory regulation of reproductive system females. This study aimed to evaluate expression PNX GPR173 by qPCR, western blot immunofluorescence assays endometrium suffering from CEH or pyometra compared clinically healthy Additionally, progesterone (P4) plasma concentrations were...
The morphology and quality of oocytes is set during oo- folliculo-genesis, completed final maturation. Early embryonic development associated with the cumulus-oocyte-complex (COC). However, current knowledge possible relationships between oocyte level proteins within oocyte, which may reflect fertilization ability, insufficient. Using western-blot analysis confocal microscopic observation, we determined levels integrin beta-2 (integrin β2) glycoprotein 3 (pZP3) protein in porcine zona...
Integrins are the major receptors within extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as adhesion, migration, cytoskeletal organization, proliferation, survival, differentiation.A vascular endothelial growth factor (VEGF) is one of most important angiogenic factors.It has been suggested expression this gene may play crucial physiological roles in reproductive organs.All investigated endometrial tissues were isolated on day 10-12...
The CC (cumulus cell) proliferation index in relation to the expression and distribution of Cdk4 Cx43 proteins, which are crucial factors for oocyte maturation, was investigated. Cumulus-oocyte complexes (COCs) were recovered from pubertal crossbred Landrace gilts treated with collagenase, separated CCs cultured standard TCM199 medium 44 h. At each step vitro cultivation (IVC) (0, 12, 24 h), a normalized assessed. protein CC-specific cellular analyzed by confocal microscopic observation....
Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3–5 and large (>5 mm) porcine follicles, the INHA INHB expression cellular localization studied. Developmentally competent (BCB+) COCs cultured for 44 h. Samples of mRNA isolated before after in vitro maturation (IVM) oocytes follicles different size RQ-PCR assay. The protein distribution within was observed by confocal microscopy. increased compared to IVM (<mml:math...
Both epidermal growth factor (EGF) and transforming (TGF) play an important physiological role in the processes of proliferation differentiation several different cell types.However, expression profiles these factors domestic bitches endometrium are still poorly recognized.The aim present study was to identify analyze differential various stages estrus cycle.Endometrial tissue from proestrus (n=17), (n=10), day 10 diestrus (n=15), 35 (n=18) anestrus (n=25) collected soon after...
Abstract Huntington's disease (HD) is a neurodegenerative polyglutamine (polyQ) resulting from the expansion of CAG repeats located in ORF huntingtin gene ( HTT ). The extent to which mutant mRNA‐driven disruptions contribute HD pathogenesis, particularly comparison dominant mechanisms related gain‐of‐function effects polyQ protein, still debatable. To evaluate this contribution vivo, we generated two mouse models through knock‐in strategy at Rosa26 locus. These expressed distinct variants...
Oocytes and somatic cumulus cells are connected by an extensive network of gap junctions. These connections contribute in a major way to oocyte maturation developmental competence. Cumulus-oocyte complexes (COCs) were cultured standard porcine IVM culture medium (TCM 199) for 44 h. The morphological classification COCs is based on the number cell layers degree their compaction, as well cytoplasm composition (homogenous, heterogeneous). obtained divided into four grades according this system....
Abstract Gap junction connections are formed by proteins which play an important role in oocyte developmental competency but there is little information on the relationship between follicle size and expression of genes encoding these proteins. The aim this study was to investigate potential association levels Cdk4 Cx43 using western blot analysis confocal microscopic observations. Cumulus-oocyte complexes (COCs) were collected from puberal gilts (n = 20) large (>5 mm), medium (3-5 small...
The influence of selected semen extenders on the motility frozen-thawed dog spermatozoa has been clearly demonstrated in several studies, although there are no reports indicating effect swim-up purification sperm viability this species mammals. Therefore, study was aimed at investigating phosphatidylserine (PS) externalization and necrosis after variable lengths time vitro incubation purification. Dog samples were collected from (i) ten dogs aged six months to 1.5 year, (ii) eight years,...
Summary The current study aimed to investigate differential expression of inhibin βA (INHβA) and βB (INHβB) in porcine oocytes before or after vitro maturation (IVM) isolated from follicles various sizes. Porcine large, medium small (40 each) were used the INHβA INHβB protein pattern using western blot analysis 44 h oocyte IVM. An increased was found collected large compared with IVM ( P < 0.001, 0.05, respectively). Similarly, higher levels observed recovered 0.01). As are expressed both...
Summary This study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well ZP3, ZP4 (ZPB) protein distribution before and after in vitro maturation (IVM) canine oocytes. The cumulus–oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches matured for 72 h TCM199 medium. COCs analysed IVM. Using real-time quantitative polymerase chain reaction (RQ-PCR), both groups of oocytes detection ZP2 profiles using confocal microscopic analysis...
Cystic endometrial hyperplasia (CEH) and pyometra are the most frequently diagnosed uterine diseases affecting bitches of different ages. Transforming growth factor beta (TGF-β) has been classified in females as a potential regulator many changes during estrous cycle or may be involved pathological disorders. The aim this study was to determine expression TGF-β1, -β2 -β3 endometrium suffering from CEH CEH–pyometra complex compared clinically healthy (control group; CG). A significantly...