A5018788244- Protein purification and stability
- Multiple Myeloma Research and Treatments
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Diet and metabolism studies
- Immune Cell Function and Interaction
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Dietary Effects on Health
- Birth, Development, and Health
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Dialysis and Renal Disease Management
- Mindfulness and Compassion Interventions
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Nutritional Studies and Diet
- COVID-19 and Mental Health
Indiana University – Purdue University Indianapolis
2025
Indiana University Health
2025
Riley Hospital for Children
2025
Center for Drug Evaluation and Research
2016-2025
Grady Memorial Hospital
2025
Colorado State University
2018-2024
Kentucky Department of Education
2024
University of Louisville
2024
Emory University
2014-2023
Adaptive Biotechnologies (United States)
2019-2023
BACKGROUND AND OBJECTIVES A Behavioral Emergency Reponse Team (BERT) is a rapid response team for behavioral emergencies, which include clinical psychiatric coping/stress reactions and physical or verbal conflicts.1 Use of BERT activations (“alerts”) in inpatient pediatric populations understudied. The objective this study to determine any differences by race individuals receiving alerts at single hospital. METHODS cross-sectional retrospective review all was conducted between January 1,...
Although the value of proteomics has been demonstrated, cost and scale are typically prohibitive, gene expression profiling remains dominant for characterizing cellular responses to perturbations. However, high-throughput sentinel assays provide an opportunity contribute at a meaningful scale. We present systematic library resource (90 drugs × 6 cell lines) proteomic signatures that measure changes in reduced-representation phosphoproteome (P100) epigenetic marks on histones (GCP). A...
Abstract Bacteria harbored in the male reproductive system may influence function and health of result developmental origins adult disease (DOHaD) effects his offspring. Such could be due to seminal fluid, which is slightly basic enriched with carbohydrates; thereby, creating an ideal habitat for microbes or a potential fluid microbiome (SFM). Using wild-type (WT) estrogen receptor-alpha (ESR1) knockout (KO) mice, we describe unique SFM whose inhabitants differ from gut microbes. The...
To investigate associations between periodontitis and chronic obstructive pulmonary disease (COPD) with without alpha-1 antitrypsin deficiency (AATD), including neutrophil functions implicated in tissue damage.The presence severity of (using two international criteria) lung were assessed 156 COPD patients AATD accounting for common confounding factors. Saliva systemic inflammatory markers measured by ELISA together migration.COPD exhibited higher prevalence (COPD 95%; 88%) than reported...
Probiotics make up a large and growing segment of the commercial market dietary supplements are touted as offering variety human health benefits. Some purported positive impacts probiotics include, but not limited to, stabilization gut microbiota, prevention gastrointestinal disorders modulation host immune system. Current research suggests that immunomodulatory effects strain-specific vary in mode action. Here, we examined properties Bacillus subtilis strain DE111 healthy population. In...
Our prior work showed that a novel microbiome resides in the seminal vesicles of wild-type and oestrogen receptor α (Esr1) knock-out mice is impacted by presence functional Esr1 genes. The fluid (SFM) may influence health reproductive status male, along with his partner offspring. A high-fat diet (HFD) alters metabolites other factors within might affect SFM. Adult (~15 weeks old) male were placed for 4 on control or fecal samples collected, bacterial DNA isolated subjected to 16s rRNA...
Viral contamination is a potential risk of using biotechnology products derived from mammalian cell lines. Therefore, to provide assurance the safety these regarding viral contamination, regulatory authorities require clearance studies assessing ability purification process clear endogenous and exogenous viruses. A database containing study data FDA submissions was initiated in 2008, an initial analysis that published 2010. Since 2010, has been updated with additional biological license...
Cardiovascular disease (CVD) is the leading cause of death in US and worldwide. By 2030 it anticipated that CVD will claim lives more than 24 million people. Throughout last decade, researchers have investigated role gut microbiota development CVD. Evidence exists for a positive correlation between Bifidobacterium vascular function, glucose tolerance, reduced systemic inflammation. Another probiotic species, Bacillus subtilis , has also been found to reduce cholesterol levels human animal...
Timely completion of genome replication is a prerequisite for mitosis, integrity, and cell survival. A challenge to this timely comes from the need replicate hundreds untranscribed copies rDNA that organisms maintain in addition required ribosome biogenesis. Replication these arrays relegated late S phase despite their large size, repetitive nature, essentiality. Here, we show that, Saccharomyces cerevisiae, reducing number repeats leads early replication, which results delaying elsewhere...
Understanding natural immunologic control over Human Immunodeficiency Virus (HIV)-1 replication, as occurs in rare long-term nonprogressors/elite controllers (LTNP/EC), should inform the design of efficacious HIV vaccines and immunotherapies. Durable LTNP/EC is likely mediated by highly functional virus-specific CD8+ T-cells. Protective Leukocyte Antigen (HLA) class I alleles, like B*27 B*57, are present most, but not all LTNP/EC, providing an opportunity to investigate features shared their...
The risk for virus contamination in biotechnology products (e.g., monoclonal antibodies, fusion proteins, or antibody-drug conjugates) derived from mammalian cell lines is a safety concern that must be evaluated the early stages of development. regulatory requirement clearance assesses capacity purification processes to remove endogenous and adventitious viruses aimed at mitigating viral risk. A database, containing study data biological license application investigational new drug...
ABSTRACT Multi‐modal anion exchange resins combine properties of both and hydrophobic interaction chromatography for commercial protein polishing may provide some viral clearance as well. From a regulatory claim standpoint, it is unclear if multi‐modal are truly orthogonal to either single‐mode or columns. To answer this, strategy solute surface assays High Throughput Screening resin in concert with scale‐down model large scale purification was employed determine the predominant binding...
We designed small-scale virus filtration models to investigate the impact of extended process times and dynamic product streams present in continuous manufacturing. Our data show that Planova 20N BioEX filters are capable effectively removing bacteriophage PP7 (>4 log) when run continuously for up 4 days. Additionally, both were able successfully a mock elution peak increased protein, salt, concentrations with only an increase pressure observed during higher protein concentration peak. These...