A5006314846- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Prostate Cancer Treatment and Research
- Burkholderia infections and melioidosis
- Radiomics and Machine Learning in Medical Imaging
- Brucella: diagnosis, epidemiology, treatment
- Medical Imaging Techniques and Applications
- Urinary and Genital Oncology Studies
- Hormonal and reproductive studies
- Galectins and Cancer Biology
- Advanced Radiotherapy Techniques
- Business Process Modeling and Analysis
- Cancer Diagnosis and Treatment
- Protein purification and stability
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Influenza Virus Research Studies
- Lung Cancer Diagnosis and Treatment
- Amino Acid Enzymes and Metabolism
- Cardiovascular Function and Risk Factors
- Tracheal and airway disorders
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
Molecular Oncology (United States)
2025
European Institute of Oncology
2023-2024
University of Milan
2013-2024
University of Milano-Bicocca
2006-2013
The outer membrane (OM) of gram-negative bacteria is an asymmetric lipid bilayer that protects the cell from toxic molecules. Lipopolysaccharide (LPS) essential component OM in most bacteria, and its structure biosynthesis are well known. Nevertheless, mechanisms transport assembly this molecule poorly understood. To date, only proteins implicated LPS MsbA, responsible for flipping across inner membrane, Imp/RlpB complex, involved targeting to OM. Here, we present evidence two Escherichia...
Lipopolysaccharide is a major glycolipid component in the outer leaflet of membrane (OM), peculiar permeability barrier Gram-negative bacteria that prevents many toxic compounds from entering cell. transport (Lpt) across periplasmic space and its assembly at Escherichia coli cell surface are carried out by transenvelope complex seven essential Lpt proteins spanning inner (LptBCFG), periplasm (LptA), OM (LptDE), which appears to operate as unique machinery. LptC an membrane-anchored protein...
ABSTRACT Lipopolysaccharide (LPS) is a major glycolipid present in the outer membrane (OM) of Gram-negative bacteria. The peculiar permeability barrier OM due to presence LPS at leaflet this that prevents many toxic compounds from entering cell. In Escherichia coli synthesized inside cell first translocated over inner (IM) by essential MsbA flippase; then, seven Lpt proteins located IM (LptBCDF), periplasm (LptA), and (LptDE) are responsible for transport across periplasmic space its...
Lipopolysaccharide (LPS) is the main glycolipid present in outer leaflet of membrane (OM) Gram-negative bacteria, where it modulates OM permeability, therefore preventing many toxic compounds from entering cell. LPS biogenesis an essential process bacteria and thus ideal target pathway for development novel specific antimicrobials. The lipopolysaccharide transport (Lpt) system responsible transporting periplasmic surface inner membrane, assembled, to cell then inserted OM. Lpt has been...
Abstract OBJECTIVES Malignant central airway obstruction (MCAO) is a condition characterized by severe dyspnoea and high mortality. Tracheobronchial stents have emerged as an effective treatment to restore patency. Our study aimed evaluate the efficacy of 3D reconstruction upper airways plan tracheo-bronchial stent insertion for MCAO. METHODS This was retrospective, single-center observational study. We analysed data patients under-going MCAO from 01/2012 01/2024. Patients undergoing with...
LptA is a periplasmic protein involved in the transport of lipopolysaccharide (LPS) from inner membrane (IM) to outer (OM) Gram-negative bacteria. Growing evidence supports model which assembles into oligomers, forming physical bridge connecting IM and OM. This work investigates assembly architecture oligomers. Circular dichroism "native" electrospray-ionization ion-mobility mass spectrometry (ESI-IM-MS) are employed test concentration dependence structural features analyze morphology...
The assembly of lipopolysaccharide (LPS) in the outer leaflet membrane (OM) requires transenvelope Lpt (lipopolysaccharide transport) complex, made Escherichia coli seven essential proteins located inner (IM) (LptBCFG), periplasm (LptA), and OM (LptDE). At IM, LptBFG constitute an unusual ATP binding cassette (ABC) transporter, composed by transmembrane LptFG cytoplasmic LptB ATPase, which is thought to extract LPS from IM provide energy for its export across cell surface. LptC a small...
Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified panel of 104 candidate antigens the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for disease melioidosis. B. pseudomallei can cause fatal sepsis endemic...
Abstract Background Protein over-expression in bacteria is still the easiest, cheapest and therefore preferred way to obtain large amounts of proteins for industrial laboratory scale preparations. Several studies emphasized importance understanding cellular molecular mechanisms triggered by protein over-production order higher yield better quality recombinant product. Almost every step leading a fully functional polypeptide has been investigated, from mRNA stability role chaperones,...
The 1.8 Å resolution crystal structure of a conserved domain the potential Burkholderia pseudomallei antigen and trimeric autotransporter BPSL2063 is presented as structural vaccinology target for melioidosis vaccine development. Since (1090 amino acids) hosts only one domain, expression/purification full-length protein proved to be problematic, domain-filtering library was generated using β-lactamase reporter gene select further domains. As result, two domains (D1 D2) were identified...
(1) Background: In the RADIOSA phase II randomized clinical trial (NCT03940235), biology task entails identification of predictive and prognostic biomarkers in context oligorecurrent, castration-sensitive prostate cancer order to distinguish polymetastatic from oligometastatic disease. This may lay groundwork for personalized treatments those patients who could really benefit metastasis-directed therapies. (2) Methods: Oligorecurrent PCa pts with three or fewer bone lymph nodal localizations...
The study aims to report the feasibility and safety of palliative hypofractionated radiotherapy targeting macroscopic bladder tumors in a monocentric cohort frail elderly cancer patients not eligible for curative treatments.
Purpose or Objective—The aim of the study is to evaluate efficacy and safety SBRT on detectable prostate bed recurrence in RT-naïve cancer patients. Materials methods: Eighty-six patients who underwent for macroscopic after prostatectomy were retrospectively included. Patients treated based mpMRI choline/PSMA PET. Results: The median time biochemical relapse (BCR) RP was 46 months, with a PSA at restaging 1.04 ng/mL. Forty-six staged PET, while ten thirty PET MRI only, respectively. Only one...