A5001996210- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Infections and bacterial resistance
- Burkholderia infections and melioidosis
- Monoclonal and Polyclonal Antibodies Research
- Plant Pathogenic Bacteria Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Advanced biosensing and bioanalysis techniques
- Brucella: diagnosis, epidemiology, treatment
- Genomics and Phylogenetic Studies
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Protein purification and stability
- Virus-based gene therapy research
- Galectins and Cancer Biology
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Microbial Fuel Cells and Bioremediation
- vaccines and immunoinformatics approaches
- RNA Research and Splicing
- Antimicrobial Peptides and Activities
- Alzheimer's disease research and treatments
Universitat Autònoma de Barcelona
2016-2025
Czech Academy of Sciences, Institute of Biotechnology
2020
Instituto de Biomedicina y Biotecnología de Cantabria
2007
Abstract Background Protein aggregation correlates with the development of several debilitating human disorders growing incidence, such as Alzheimer's and Parkinson's diseases. On biotechnological side, protein production is often hampered by accumulation recombinant proteins into aggregates. Thus, methods to anticipate properties polypeptides receiving increasing attention. AGGRESCAN a web-based software for prediction aggregation-prone segments in sequences, analysis effect mutations on...
Abstract Recent studies portend a rising global spread and adaptation of human- or healthcare-associated pathogens. Here, we analyse an international collection the emerging, multidrug-resistant, opportunistic pathogen Stenotrophomonas maltophilia from 22 countries to infer population structure clonality at level. We show that S. complex is divided into 23 monophyletic lineages, most which harbour strains all degrees human virulence. Lineage Sm6 comprises highest rate human-associated...
The fully de novo design of protein building blocks for self-assembling as functional nanoparticles is a challenging task in emerging nanomedicines, which urgently demand novel, versatile, and biologically safe vehicles imaging, drug delivery, gene therapy. While the use viruses virus-like particles limited by severe constraints, generation protein-only nanocarriers progressively reachable engineering protein-protein interactions, resulting blocks. In particular, end-terminal cationic...
Prions are a particular type of amyloids with the ability to self-perpetuate and propagate in vivo. Prion-like conversion underlies important biological processes but is also connected human disease. Yeast prions best understood transmissible amyloids. In these proteins, prion formation from an initially soluble state involves structural conversion, driven, many cases, by specific domains enriched glutamine/asparagine (Q/N) residues. Importantly, sharing this compositional bias present...
The impact of cell factory quality control on material properties is a neglected but critical issue in the fabrication protein biomaterials, which are unique merging structure and function. molecular chaperoning conformational status revealed here as potent instructor macroscopic self-assembling, cell-targeted nanoparticles, including biodistribution upon vivo administration. As service to our authors readers, this journal provides supporting information supplied by authors. Such materials...
Aims: Arginine(R)-rich cationic peptides are powerful tools in drug delivery since, alone or when associated with polyplexes, proteins chemicals, they confer DNA condensation, membrane translocation and blood–brain barrier crossing abilities. The unusual stability high vivo performance of their drugs suggest a particulate organization R(n) complexes, which this study aimed to explore. Materials & methods: We have analyzed the biological model, R9-containing green fluorescent protein by...
Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a potentially fatal infection that endemic in Southeast Asia and Northern Australia poorly controlled antibiotics. Research efforts to identify antigenic components for melioidosis vaccine have led identification of several proteins, including subunits forming flagella mediate bacterial motility, host colonization, virulence. This study focuses on B. pseudomallei flagellar hook-associated protein (FlgK(Bp)),...
Abstract Summary: The Quest for Orthologs (QfO) is an open collaboration framework experts in comparative phylogenomics and related research areas who have interest highly accurate orthology predictions their applications. We here report highlights discussion points from the QfO meeting 2015 held Barcelona. Achievements recent years established a basis to support developments improved prediction explore new approaches. Central effort proper benchmarking of methods services, as well design...
Abstract In Pseudomonas aeruginosa , Ttg2D is the soluble periplasmic phospholipid-binding component of an ABC transport system thought to be involved in maintaining asymmetry outer membrane. Here we use crystallographic structure at 2.5 Å resolution reveal that this protein can accommodate four acyl chains. Analysis available structures orthologs shows they conform a new substrate-binding-protein structural cluster. Native and denaturing mass spectrometry experiments confirm Ttg2D, produced...
Stenotrophomonas maltophilia are opportunistic, multi-drug-resistant Gram-negative pathogens increasingly prevalent in clinical settings. Quorum sensing (QS) systems play a crucial role their pathogenesis, coordinating bacterial populations and enabling interactions within polymicrobial communities. While not the primary QS mechanism S. maltophilia, these bacteria can respond to acyl-homoserine lactone (AHL)-type autoinducers. Some isolates exhibit AHL-quorum quenching activity, though...
Around 1% of human proteins are predicted to contain a disordered and low complexity prion-like domain (PrLD). Mutations in PrLDs have been shown promote transition towards an aggregation-prone state several diseases. Recently, we that algorithm considers the effects mutations on composition, as well localized amyloid propensity can predict impact these amino acid changes protein intracellular aggregation. In this application note, implement concept into AMYCO web server, refined forecasts...
The pathogenicity of Stenotrophomonas maltophilia is regulated in part by its quorum sensing (QS) system. main QS signaling molecule S. known as diffusible signal factor (DSF), and the rpf gene cluster responsible for synthesis perception. Two variants have been previously described, rpf-1 rpf-2, which differ basically conditions under DSF produced. Here, correlations between variant antibiotic susceptibility, LPS electrophoretic profiles virulence-related phenotypes were evaluated a...
Fluorescently labeled bacterial cells have become indispensable for many aspects of microbiological research, including studies on biofilm formation as an important virulence factor various opportunistic bacteria environmental origin such Stenotrophomonas maltophilia. Using a Tn7-based genomic integration system, we report the construction improved mini-Tn7 delivery plasmids labeling S. maltophilia with sfGFP, mCherry, tdTomato and mKate2 by expressing their codon-optimized genes from...
Stenotrophomonas maltophilia are ubiquitous Gram-negative bacteria found in both natural and clinical environments. It is a remarkably adaptable species capable of thriving various environments, thanks to the plasticity its genome diverse array genes that encode wide range functions. Among these functions, one notable trait remarkable ability resist antimicrobial agents, primarily through mechanisms regulate diffusion across cell membranes. We have investigated Mla ABC transport system S. ,...
Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, the structure functionality can be adapted by genetic engineering. Furthermore, strategies tools for protein production have been developed refined steadily more than 30 years. However, conformation therefore activity might sensitive conditions. Here, we explored whether downstream...
Stenotrophomonas maltophilia is an environmental bacterium as well emerging opportunistic multidrug-resistant pathogen. They use the endogenous diffusible signal factor (DSF) quorum sensing (QS) system to coordinate population behavior and regulate virulence processes but can also respond exogenous N-acyl-homoserine lactone (AHL) signals produced by neighboring bacteria. The effect of these QS on global gene expression this species remains, however, unknown. Whole-transcriptome sequencing...
Aim & Methods: We have produced two chimerical peptides of 10.2 kDa, each contain four biologically active domains, which act as building blocks protein-based nonviral vehicles for gene therapy. In solution, these tend to aggregate amorphous clusters more than 1000 nm, while the presence DNA promotes their architectonic reorganization mechanically stable nanometric spherical entities approximately 80 nm that penetrate mammalian cells through arginine–glycine–aspartic acid cell-binding...