A5063444977- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Free Radicals and Antioxidants
- Phytochemicals and Antioxidant Activities
- Antioxidant Activity and Oxidative Stress
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Chromatography in Natural Products
- Advancements in Transdermal Drug Delivery
- Pharmaceutical and Antibiotic Environmental Impacts
- Analytical Chemistry and Chromatography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Natural Compound Pharmacology Studies
- Monoclonal and Polyclonal Antibodies Research
- Multiple Myeloma Research and Treatments
- Effects and risks of endocrine disrupting chemicals
- Advanced biosensing and bioanalysis techniques
- Phytochemistry and Biological Activities
- Electrochemical Analysis and Applications
- Cancer Mechanisms and Therapy
- Wastewater Treatment and Reuse
- Essential Oils and Antimicrobial Activity
- Advanced Drug Delivery Systems
- Dye analysis and toxicity
- Advanced Chemical Sensor Technologies
Universidade do Porto
2014-2024
Rede de Química e Tecnologia
2014-2024
IPO Porto
2023-2024
The analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate different mathematical approaches often lacking correct elucidation kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression values as area under fluorescein (FL) decay curves (AUC) lag time are critically compared. This multi-parametric showed extension beyond period for caffeic...
Background and Aims The addition of oenological tannins during winemaking is a longstanding technological practice. To assist such practice, knowledge their antioxidant properties an asset for understanding role in redox processes occurring wine. Hence, the aim this work was to assess profile from several botanical sources, which are usually applied at different steps. Methods Results Several assays, including ability reduce copper(II) assay (CUPRAC), scavenging capacity against...
The evaluation of encapsulation efficiency is a regulatory requirement for the characterization drug delivery systems. However, difficulties in efficiently separating nanomedicines from free may compromise achievement accurate determinations. Herein, ultrafiltration was exploited as separative strategy towards methotrexate (MTX) nanostructured lipid carriers and polymeric nanoparticles. effect experimental conditions such pH amount surfactant present media addressed aiming at selection...
The presence of endocrine-disrupting compounds (EDCs) in water poses a significant threat to human and animal health, as recognized by regulatory agencies throughout the world. Yeast estrogen screen (YES) assay is an excellent method evaluate these due its simplicity capacity assess bio-accessible forms/fractions compounds. In compound with estrogenic activity, Saccharomyces cerevisiae cells, containing lacZ reporter gene encoding enzyme β-galactosidase, are induced, synthesised, released...
Searching of new enantiomerically pure chiral derivatives xanthones (CDXs) with potential pharmacological properties, particularly those anti-inflammatory activity, has remained an area interest our group. Herein, we describe in silico studies and vitro inhibitory assays cyclooxygenases (COX-1 COX-2) for different enantiomeric pairs CDXs. The evaluation the activities was performed by using COX Inhibitor Screening Assay Kit. Docking simulations between small molecules (CDXs; known ligands...
Ozonation followed by ultrafiltration (O3 + UF) was employed at pilot scale for the treatment of secondary urban wastewater, envisaging its safe reuse crop irrigation. Chemical contaminants emerging concern (CECs) and priority substances (PSs), microbial load, estrogenic activity, cell viability cellular metabolic activity were measured before immediately after O3 UF treatment. The load also evaluated one-week storage treated water to assess potential bacteria regrowth. Among organic...
Immunochemical techniques are the workhorse for sample enrichment and detection of a large variety analytes. In contrast to classical microtiter plate-based assays, microparticles next generation solid support, as they promote automation immunoassays using flow-based techniques. Antibody immobilization is crucial step, these reagents expensive, inefficient coupling can result in low sensitivities. This paper proposes general procedure efficient antibodies onto TentaGel particles, via...
Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose–response correlations evaluating reproducibility manufacturing process. Still, faster simpler procedures, dismissing skilled operators post-analysis conversions are needed quantify research operations, support result validation. Herein, a miniaturized automated ensemble...
The ABTS˙+ assay, based on reduction of the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation, has been automated using flow injection methods, but biased results are often obtained because scavenging reaction is not complete upon detection, providing underestimated antioxidant values for samples containing slow reacting compounds. In this work a mesofluidic lab-on-valve (LOV) system was developed to provide fast determination total capacity in miniaturized fashion. sample...
Searching for new enantiomerically pure chiral derivatives of xanthones (CDXs) with potential pharmacological properties has remained an area interest our group, namely those anti-inflammatory activity [1,2]. Herein, we describe in silico studies and vitro inhibitory assays different enantiomeric pairs CDXs. The evaluation the inhibition cyclooxygenases (COX-1 COX-2) activities was performed by using COX Inhibitor Screening Assay Kit. Docking simulations between small molecules (CDXs, known...