A5014889005- Fungal Biology and Applications
- Phytochemicals and Antioxidant Activities
- Natural product bioactivities and synthesis
- Natural Antidiabetic Agents Studies
- Microbial Natural Products and Biosynthesis
- Sesquiterpenes and Asteraceae Studies
- Polymer Surface Interaction Studies
- Essential Oils and Antimicrobial Activity
- Bee Products Chemical Analysis
- Infectious Aortic and Vascular Conditions
- Porphyrin and Phthalocyanine Chemistry
- Electrospun Nanofibers in Biomedical Applications
- Genomics, phytochemicals, and oxidative stress
- Tannin, Tannase and Anticancer Activities
- Heat shock proteins research
- Traditional and Medicinal Uses of Annonaceae
- Orthopedic Infections and Treatments
- Retinoids in leukemia and cellular processes
- Endoplasmic Reticulum Stress and Disease
- Polysaccharides and Plant Cell Walls
- Papaya Research and Applications
- Graphene and Nanomaterials Applications
- thermodynamics and calorimetric analyses
- Meat and Animal Product Quality
- Oxidative Organic Chemistry Reactions
Maria Curie-Skłodowska University
2017-2025
Institute of Microbiology and Biotechnology
2022
Amphotericin B is an antibiotic used in pharmacotherapy of life-threatening mycotic infections. Unfortunately, the applicability this associated with highly toxic side effects. In order to understand molecular mechanisms underlying toxicity amphotericin patients, two cell lines, human normal colon epithelial cells (CCD 841 CoTr) and adenocarcinoma (HT-29) were cultured presence drug imaged application fluorescence lifetime imaging microscopy Raman scattering microscopy. The results viability...
The aim of this study was to investigate the anticancer and antioxidant activities low molecular weight subfractions isolated from secondary metabolites produced by wood degrading fungus Cerrena unicolor. Human colon cancer cells (stage I) HT-29 human normal epithelial CCD 841 CoTr were used in research. present demonstrated that exhibited inhibitory activity towards at a concentration range 25-200 μg/mL. All 6 inhibited proliferation down 47.5-9.2% highest concentrations dose-dependent...
Colorectal cancer is one the most lethal cancers worldwide. Since chemotherapy burdened with harmful effects, agents capable of enhancing chemotherapeutic effect are being sought. Ursolic acid (UA) and oleanolic (OA) were analyzed for such properties. The aim study was to evaluate ability UA OA administered individually in combination each other and/or a cytostatic drug camptothecin-11 (CPT-11) limit viability migration colorectal cells.The cytotoxic UA, CPT-11 impact on normal cell rate...
Various phytochemical studies have revealed that jackfruit (Artocarpusheterophyllus Lam.) is rich in bioactive compounds, including carotenoids, flavonoids, volatile acids, tannins, and lectins. The aim of the study was to analyze biological activity water-soluble polysaccharide (WSP) isolated from assess its immunomodulatory, cytotoxic, anti-oxidative effects on human colon carcinoma cells vitro. neutral red (NR) uptake assay no toxic influence polymer viability tumor (HT29 SW620). After 24...
Background: Cancer is one of the main causes death worldwide. Contemporary therapies, including chemo- and radiotherapy, are burdened with severe side effects. Thus, there exists an urgent need to develop therapies that would be less devastating patient’s body. Such novel approaches can based on anti-tumorigenic activity particular compounds or may involve sensitizing cells chemotherapy radiotherapy reducing side-effects regular treatment. Objective: Natural-derived becoming more popular in...
Abstract The antitumour activity of the oxygenated derivatives pinene is still poorly understood. We hypothesized that pinene, (−)‐myrtenol, (−)‐myrtanol, and (−)‐myrtenal, would express different effects on human colon tumour cells normal intestinal in vitro . For cytotoxic nitric oxide ( NO x) assays, HT 29) epithelial CCD 841 CoTr) were incubated for 24 h with 0.1–5.0 mM terpenoids. x level was determined pre‐incubated or not lipopolysaccharide LPS ). All three terpenoids decreased...
Abstract The modification of the surgical polypropylene mesh and polytetrafluoroethylene vascular prosthesis with cecropin A (small peptide) puromycin (aminonucleoside) yielded very stable preparations modified biomaterials. main emphasis was placed on analyses their antimicrobial activity potential immunomodulatory non-cytotoxic properties towards CCD841 CoTr model cell line. Cecropin did not significantly affect viability or proliferation CCD 841 cells, regardless its soluble immobilized...
The present study aimed to demonstrate
Arnica montana L. is one of Europe’s endemic endangered medicinal plants, with diverse biological activities commonly used in medicine, pharmacy, and cosmetics. Its flower heads are a rich source raw material, antibacterial, antifungal, antiseptic, anti-inflammatory, antiradical, antioxidant, antitumor properties. The objective the present study was (i) to characterize chemical composition A. plants cultivated under nitrogen fertilization, (ii) identify impact fertilization extraction method...
Collagen-sealed polyester (PET) prostheses are commonly used in reconstructive vascular surgery due to their self-sealing properties. To prevent post-surgical infection, different modification methods have been tested but so far none showed long-term satisfactory efficiency. For this reason, the present study, a commercial collagen-sealed PET prosthesis was coated by highly adhesive poly (L-DOPA) layer maintaining sealing protein without losing original properties and functionality. This...
Colorectal cancer (CRC) is one of the most common and deadliest cancers worldwide. According to GLOBOCAN (WHO) report in 2020, nearly 2 million patients were diagnosed globally. Despite advances diagnosis therapy, CRC remains a global challenge. Recently, attention has been paid tumor microenvironment (TME), which constitutes significant part mainly includes various immune cells, fibroblasts, vascular extracellular elements, such as matrix (ECM). Many components stroma initially exert an...
Anti-proliferative and antioxidant activities of Pleurotus pulmonarius P. citrinopileatus water-soluble polysaccharides were examined using human colon adenocarcinoma (HT-29) normal colonic epithelial (CCD841 CoTr) cell lines in vitro. Polysaccharides prepared by cold hot water extraction methods chemically characterized. The monosaccharide analysis showed that all the preparations contained hexoses: mannose, glucose, galactose, 3-O-methyl-hexose (identified as 3-O-methyl-galactose) well...
In this study, the influence of two subfractions (with previously proven anti-cancer properties) isolated from wood rot fungus Cerrena unicolor on formation a fibrin clot was investigated in context potential use as glue and sealant enhancers wound healing agents. With ROTEM thromboelastometry, we demonstrated that, presence fibrinogen thrombin, S6 fraction accelerated clot, had positive effect its elasticity modulus, enhanced degree cross-linking. The S5 alone showed no coagulation process;...
In recent years, new sources of secondary metabolites (SMs) in medicinal plants have been identified, and the introduction these into field conditions has carried out to obtain chemically diverse standardized raw material (RM). An example is mountain arnica Arnica montana L., one Europe’s endemic endangered plant species, commonly used pharmacy, cosmetics, medicine. Its inflorescences (Arnicae flos) are characterized by anti-inflammatory, antiradical, antioxidant, antibacterial, antifungal,...
Potentilla alba (Rosaceae) has been used in the traditional folk medicine for several hundred years. However, data on cytotoxicity effects of this plant are limited [1],[2]. In study following extracts from aerial parts P. were used: MeOH (PAL1), 50% MEOH (PAL2), water (PAL3), Et2O (PAL4), EtOAc (PAL5), n-BuOH (PAL6) and 70% acetone (PAL7). MTT NR assays assessing cells viability used. The tests carried out human colon tumour (HT29) normal epithelial (CCD 841 CoTr) cell cultures. uptake...
The aim of this research was to determine the biological activity (1→3),(1→4)-α-D-glucan from mango (Mangifera indica L.) fruits on human colon carcinoma cells HT29 (Duke's A) and SW620 C).Many natural substances have been described show anti-tumor or limit dissemination transformed cells.Among them, there are polysaccharides, which macromolecules consisting different amounts monosaccharides linked by glycosidic bonds.One such polymers is water-soluble fruits.The isolated did not induce a...