A5014922771- Ovarian cancer diagnosis and treatment
- Endometriosis Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Uterine Myomas and Treatments
- Endometrial and Cervical Cancer Treatments
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Cancer Mechanisms and Therapy
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Endoplasmic Reticulum Stress and Disease
- Psoriasis: Treatment and Pathogenesis
- Agriculture, Plant Science, Crop Management
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- Vitamin D Research Studies
- Cancer Cells and Metastasis
- Heat shock proteins research
- Chemokine receptors and signaling
- Sirtuins and Resveratrol in Medicine
Medical University of Lublin
2015-2024
University of Economics and Innovation
2013
National Center for Tumor Diseases
2012
Instytut Medycyny Wsi im. Witolda Chodźki
2003-2007
Glioblastoma multiforme is the most aggressive primary brain tumour. At cellular and molecular levels, several mechanisms responsible for apoptosis or autophagy induction are blocked. Identification of targets stimulating cells to initiate programmed cell death should be performed therapeutic purposes. A promising solution combination temozolomide quercetin. The aim our study was evaluate effect both drugs, applied alone in combinations, on human glioblastoma T98G cells. Our results clearly...
Myeloid-derived suppressor cells (MDSCs) expansion is a hallmark of cancer. Three major MDSC subsets defined as monocytic (M)-MDSCs, polymorphonuclear (PMN)-MDSCs and early stage (e)MDSCs can be revealed in human diseases. However, the clinical relevance immunosupressive pattern these epithelial ovarian cancer (EOC) are unknown. Therefore, we performed comprehensive analysis each subset factors peripheral blood (PB), peritoneal fluid (PF) tumor tissue (TT) samples from EOC integrated this...
The aim of the present study was to investigate effect sorafenib and quercetin on induction apoptosis autophagy in human anaplastic astrocytoma (MOGGCCM) glioblastoma multiforme (T98G) cell lines. In MOGGCCM cells, initiated mainly apoptosis, mediated by mitochondrial pathway with membrane permeabilization, cytochrome c release cytoplasm, activation caspase 9 3. Additional incubation potentiated pro-apoptotic properties sorafenib. T98G observed most frequently after treatment. It accompanied...
The study was undertaken to evaluate macrophage-derived chemokine (CCL22) levels in the peritoneal fluid (PF) and plasma of patients with ovarian cancer (n = 93) relation regulatory T cells (Tregs; n 75). CCL22 concentrations were significantly higher epithelial (EOC) than benign tumors-serous cystadenoma 32). There no difference EOC compared non-cancer healthy volunteers 10). significant differences PF based on tumor grade. However, women stage IV FIGO (International Federation...
Ovarian cancer (OC) is usually diagnosed at an advanced stage and related with poor prognosis. Despite numerous studies, the pathogenesis of OC still unknown. Recent studies indicate role immune system in development spread OC. The identification factors mechanisms involved that process their modulation crucial for creating effective antitumor therapy. We investigated potential Th17 cells patients ( n = 71) by analyzing frequencies three different environments, i.e., peripheral blood (PB),...
The aim of the study was to investigate anticancer potential LY294002 (PI3K inhibitor) and temozolomide using glioblastoma multiforme (T98G) anaplastic astrocytoma (MOGGCCM) cells. Apoptosis, autophagy, necrosis, granules in cytoplasm were identified microscopically (fluorescence electron microscopes). mitochondrial membrane studied by flow cytometry. activity caspases 3, 8, 9 Akt evaluated fluorometrically, while expression Beclin 1, PI3K, Akt, mTOR, caspase 12, Hsp27 determined...
The latest literature demonstrates the predominant role of programmed cell death axis (PD-1/PD-L1/PD-L2) in ovarian cancer (OC) pathogenesis. However, data concerning this issue is ambiguous. Our research aimed to evaluate clinical importance PD-L1/PD-L2 expression OC environments. We evaluated patients (n = 53). analysis was performed via flow cytometry on myeloid (mDCs) and plasmacytoid dendritic cells (pDCs) monocytes/macrophages (MO/MA) peripheral blood, peritoneal fluid (PF), tumor...
Data on the possible role of peritoneal fluid free radical-mediated oxidative damage in pathogenesis endometriosis still remains inconsistent. The aim study was to determine iron metabolism markers and their influence stress arameters women with endometriosis.110 119 patients benign ovarian cysts were included study. All visible aspirated during laparoscopy from anterior posterior cul-de-sacs. under direct vision avoid blood contamination. Haemoglobin, iron, total status, antioxidant status...
Endometrial cancer (EC) is one of the most common malignant tumors among women in 21st century, whose mortality rate increasing every year. Currently, diagnosis EC possible only after a biopsy. However, it necessary to find new biomarker that will help both and treatment non-invasive way. Circular RNAs (circRNAs) are small, covalently closed spherical stable long non-coding (lncRNAs) molecules, which abundant body fluids human tissues expressed various ways. Considering molecular...
Ovarian cancer remains the most lethal gynecologic malignancy. This is due to lack of effective screening, diagnosis predominance in late stage disease, a high recurrence rate after primary therapy, and poor treatment response platinum-resistant tumor. Thus, unique biomarkers, predictive individual disease course, prognosis are urgently needed. The aim our study was assess clinicopathological significance plasma, peritoneal fluid, tumor tissue levels mesothelin epithelial ovarian patients....
In the recent years, coumarin bioactive compounds have been identified to posess anticancer properties. Therefore, aim of present study was investigate for first time efficacy osthole, umbelliferone, esculin, and 4-hydroxycoumarin, alone in combination with Temozolomide, elimination deadly brain tumors, anaplastic astrocytoma (AA) glioblastoma multiforme (GBM) cells via programmed death. Our results indicated that 4-hydroxycoumarin initiated mainly apoptosis T98G MOGGCCM cells. Osthole most...
The etiopathogenesis of endometriosis still remains unknown. Recent data provide new valuable information concerning the role oxidative stress in pathophysiology disease. It has been proved that levels different lipid peroxidation end products are increased both peritoneal fluid (PF) and serum endometriotic patients. We assessed concentration oxidized low-density lipoproteins (oxLDL) PF 110 women with stages 119 serous (n = 78) or dermoid 41) ovarian cysts, as reference groups. oxLDL were...
Abstract Gliomas are aggressive brain tumors with very high resistance to chemotherapy throughout the overexpression of multiple intracellular survival pathways. Therefore, aim present study was investigate for first time anticancer activity LY294002, phosphatidylinositol 3-kinase (PI3K) inhibitor and sorafenib, rapidly accelerated fibrosarcoma kinase (Raf) in elimination human glioma cells by programmed cell death. MOGGCCM (anaplastic astrocytoma, III) T98G (glioblastoma multiforme, IV)...