A5087210110- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Acute Lymphoblastic Leukemia research
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Multiple Myeloma Research and Treatments
- Phagocytosis and Immune Regulation
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- Diabetes and associated disorders
- Psoriasis: Treatment and Pathogenesis
- MicroRNA in disease regulation
- Endometriosis Research and Treatment
- Immune Response and Inflammation
- Multiple Sclerosis Research Studies
- Proteoglycans and glycosaminoglycans research
- Cell death mechanisms and regulation
- Advanced Breast Cancer Therapies
Medical University of Lublin
2016-2025
Instytut Medycyny Wsi im. Witolda Chodźki
2011
University Medical Center
2008
Programmed death-1 (PD-1) is an immunoreceptor predominantly expressed on exhausted T cells, which through interaction with its ligand (PD-L1), controls peripheral tolerance by limiting effector functions of lymphocytes. qRT-PCR for PD-1, PD-L1 and their splicing forms as well flow cytometric assessment surface expression was performed in a cohort 58 chronic lymphocytic leukemia (CLL) patients. In functional studies, we assessed the influence proliferative response leukemic B-cells induced...
Antigen targeted immunotherapies might represent a novel treatment for B-cell chronic lymphocytic leukemia (B-CLL). We screened the mRNA expression of tumor-associated antigens (TAAs) from literature (fibromodulin, survivin, OFA-iLRP, BAGE, G250, MAGE1, PRAME, proteinase, syntaxin, hTERT, WT-1) and TAAs defined previously by serological analysis cDNA libraries leukemic cells (PINCH, HSJ2, MAZ, MPP11, RHAMM/CD168, NY-Ren60). Peripheral blood mononuclear 43 B-CLL patients 20 healthy volunteers...
Th17 cells, a recently discovered subset of T helper cells that secrete IL-17A, can affect the inflammation process autoimmune and cancer diseases development. The purpose this study was to evaluate role IL17A in biology CLL. group included 294 untreated CLL patients different clinical stages. Here, we show higher IL-17A values were associated with less advanced stage cells' percentages PB lower who died due during follow-up (as compared surviving patients) responding first-line therapy...
The study was undertaken to evaluate macrophage-derived chemokine (CCL22) levels in the peritoneal fluid (PF) and plasma of patients with ovarian cancer (n = 93) relation regulatory T cells (Tregs; n 75). CCL22 concentrations were significantly higher epithelial (EOC) than benign tumors-serous cystadenoma 32). There no difference EOC compared non-cancer healthy volunteers 10). significant differences PF based on tumor grade. However, women stage IV FIGO (International Federation...
In alcoholic liver cirrhosis, normal cells are replaced by scar tissue (fibrosis). Liver fibrosis is a dynamic process in which activated hepatic stellate involved the synthesis of matrix proteins and regulation degeneration. The aim presented study was to assess usefulness MMP-2, MMP-8 MMP-9 as diagnostic markers disease. Sixty patients with cirrhosis were randomly enrolled during hospitalization departments hospitals from Lublin Region eastern Poland. stage estimated according...
Recent studies indicate that bone marrow stromal cells (BMSCs) derived from patients with multiple myeloma (MM) differ those of healthy donors in their expression extracellular matrix compounds and cytokine production. It is not known whether these abnormalities are primary or acquired by BMSCs on contact MM cells.Interleukin (IL)-6, IL-11, IL-10, tumor necrosis factor (TNF)-alpha production CD166+ mesenchymal the CD38+/CD138+ RPMI8226 cell line cultivated vitro monocultures co-cultivated...
The present study aimed to investigate the presence of T regulatory cells (Tregs) in peripheral blood (PB) and peritoneal fluid (PF) females with endometriosis. included 42 patients who underwent laparoscopy between 2010 2011. Flow cytometry was used determine percentage Tregs PF PB patients. No significant difference observed endometriosis group compared those control (9.1±5.4 vs. 9.1±3.8%) or (6.5±3.2 6.5±3.7%). However, found be higher groups, but significance only group. Furthermore, no...
The aim of the study was to evaluate role Interleukin-17 (IL-17), Interleukin-23 (IL-23), and transforming growth factor-β (TGF-β) in pregnancy complicated by placental insufficiency normal pregnancy.The comprised 34 patients with fetal restriction (FGR) associated preeclampsia (PE), as well 35 healthy pregnant women. concentrations IL-17, IL-23, TGF-β sera from maternal peripheral blood were determined an immunoenzymatic assay.There higher IL-17 group when compared controls. In...
Background/Objectives. The current study explores the impact of CLL on γδ T cells and, in an attempt to better understand sources immunosuppression, assesses M-MDSCs vitro. Methods. included 163 patients and 34 healthy volunteers. were screened with flow cytometry, including NKG2D, Fas, FasL, TRAIL staining. Additionally, deepen understanding immunosuppressive T, a set vitro co-cultures was performed. Results. RNAseq revealed significant, though relatively minor, changes transcriptome....
Chronic lymphocytic leukaemia (CLL) is a haematological malignancy primarily affecting older adults, characterised by the proliferation of functionally impaired B lymphocytes with abnormal expression CD5, typical T cell marker. The current study investigates cytotoxicity-related receptors (CD16, CD56, CD57, CD69) and checkpoint (LAG-3) on γδ cells in CLL patients. Sixty-nine treatment-naive patients fourteen healthy controls were recruited. Flow cytometry analysis revealed that had higher...
Type 1 diabetes mellitus is a result of autoimmune destruction pancreatic insulin producing β -cells and so far it can be cured only by injection, pancreas transplantation, or islet cells’ transplantation. The methods are, however, imperfect have lot disadvantages. Therefore new solutions are needed. best one would the use differentiated mesenchymal stem cells (MSCs). In present study, we investigated potential bone marrow-derived MSCs line for in vitro differentiation into (IPSs). We...
Chronic lymphocytic leukemia (CLL) is characterized by the peripheral accumulation of neoplastic B cells and frequently complicated systemic immunosuppression associated with an impairment in T lymphocyte activation. We hypothesized that expression immune checkpoint suppressors attenuator (BTLA) cytotoxic antigen (CTLA-4) disturbed both subpopulations CLL. The CTLA-4 BTLA mRNA was determined real-time PCR, while protein (surface or intracellular) estimated BTLA+ lymphocytes flow cytometry....