The aim of this study is to investigate in vitro the anticancer, antioxidant, and antibacterial activities three low molecular weight subfractions I, II III isolated from secondary metabolites produced by wood degrading fungus Cerrena unicolor. present demonstrated that exhibited strongest inhibitory activity towards breast carcinoma cells MDA-MB-231, prostatic PC3, cancer MCF7 with half-maximal concentration (IC50) value 52,25 μg/mL, 60,66 54,92 respectively. highest percentage inhibition...

Three bioactive fractions, extracellular laccase (ex-LAC), crude endopolysaccharides (c-EPL), and a low molecular subfraction of secondary metabolites (ex-LMS), were isolated from the idiophasic cultures white rot fungus Cerrena unicolor . For first time, we determined antioxidant properties these samples by chemiluminometric measurement (a) assessment scavenging effect on ABTS (b) DPPH reduction rate (c). The highest reducing capability was found for ex-LMS fraction: 39–90% (a), 20–90% (b),...

Chronic lymphocytic leukemia (CLL) is the most commonly observed adult hematological malignancy in Western countries. Despite fact that recent improvements CLL treatment have led to an increased percentage of complete remissions, remains incurable disease. Cerrena unicolor a novel fungal source highly active extracellular laccase (ex‑LAC) currently used industry. However, best our knowledge, no reports regarding its anti‑leukemic activity been published thus far. In present study, it was...

The aim of this study was to investigate the anticancer and antioxidant activities low molecular weight subfractions isolated from secondary metabolites produced by wood degrading fungus Cerrena unicolor. Human colon cancer cells (stage I) HT-29 human normal epithelial CCD 841 CoTr were used in research. present demonstrated that exhibited inhibitory activity towards at a concentration range 25-200 μg/mL. All 6 inhibited proliferation down 47.5-9.2% highest concentrations dose-dependent...

Abstract Fomitopsis officinalis is a medicinal mushroom used in traditional European eighteenth and nineteenth century folk medicine. Fruiting bodies of F. were collected from the natural environment Świętokrzyskie Province with consent General Director for Environmental Protection Warsaw. Mycelial cultures obtained fragments fruiting bodies. The taxonomic position mycelium was confirmed using PCR method. presence organic compounds determined by HPLC–DAD analysis. Bioelements AF-AAS....

Plants and fungi are known as a valuable source of natural medicines used in the treatment various diseases. Many them to treat human animal gastrointestinal diseases caused by parasites. The aim this study was investigate for first time antinematode properties extracellular low-molecular subfractions (ex-LMS) obtained from liquid growth medium idiophasic Cerrena unicolor cultures. fungal fractions were isolated according procedure previously described Jaszek et al. vitro tests performed...

Introduction and objective Colorectal cancer takes an increasing toll every year. Despite the dynamic development of pharmacology, there is still no drug that would be strong enough to cause apoptosis cells, but at same time free from numerous side effects. Taking traditional Eastern medicine into account, studies were carried out using extract - laccase (LAC) a medical mushroom called Cerrena unicolor on CT-26 colon cells. Preliminary cytotoxicity tests have already confirmed examined...

Ganoderma lucidum is a well-known medicinal mushroom with long history of use. This study was designed to assess the anticonvulsant potential an aqueous extract from cultured G. mycelium in 3 acute seizure models: timed intravenous pentylenetetrazole infusion, maximal electroshock threshold, and 6-Hz-induced psychomotor tests mice. Moreover, antidepressant-like anxiolytic-like effects were evaluated using forced swim test elevated plus maze mice, respectively. No changes thresholds threshold...

The effect of menadione (MQ; 2-methyl-1,4-naphtoquinone), a superoxide-generating agent, on the natural biodegradation system in medicinal white rot fungus Phellinus pini was determined. While measuring activities extracellular manganese-dependent peroxidase (MnP) and intracellular chitinase, it found that application MQ (0.75 mM) distinctly stimulated these enzymes comparison to control values (without MQ). Using capillary electrophoresis (CE) method, an increase oxalic acid (OXA)...

The main purpose of this paper was to determine the effect biochemical modification epoxy adhesive compounds on mechanical properties hot-dip galvanized steel sheet DX51+Z275 joints. adhesives (resin and curing agent) were biochemically modified by lyophilized fungal metabolites (in form fractions or materials preparation containing low molecular weight secondary lignocellulose-degrading white rot fungi (WRF) Pycnoporus sanguineus (L.) Murrill prepared two methods). (epoxy resin Epidian 53...

The antioxidative and antibacterial properties of low-molecular-weight secondary metabolite subfractions (ex-LMS) from cultures Pycnoporus sanguineus cultivated under different temperature conditions (25°C [ex-LMSa] 30°C [ex-LMSb]) were assessed. studied using chemiluminometric measurement, an ABTS assay, a DPPH reduction rate assay with Trolox ascorbic acid as the control. values noted for ex-LMSb significantly higher than those ex-LMSa: 97%, 52%, 31% respectively, at concentration 50...

A new strain of Rhizomucor variabilis producing an active extracellular lipase was identified and characterized in the present studies. The culture conditions were optimized highest production amounting to 136 U/mL achieved after 4 days cultivation. optimum pH (5.5) temperature (28 °C) determined as best for R. production. isolated enzyme preparation exhibited maximum activity at 40 °C 8.0. Lipase from stable up 50 during 2 H retaining 80% its initial activity. highly range 7.0-9.0....

Light influences developmental pathways in fungi. Recent transcriptomic and biochemical analyses have demonstrated that light the metabolism of a white-rot basidiomycete Cerrena unicolor. However, expression profile genes involved growth development, or micromorphological observations mycelium response to variable lighting culturing media, not performed. We aim reveal effect nutrients on C. unicolor potential relationship between culture medium conditions fungus structures. Confocal laser...

The present study aimed to demonstrate

Three bioactive fractions isolated from Cerrena unicolor cultures-crude endopolysaccharide (c-EPS), laccase, and a subfraction of low-molecular weight secondary metabolites-were used to determine potential cytotoxic effects on the mouse melanoma B16-F10 cell line (American Type Culture Collection CRL-6475). The results obtained prove that all examined exhibited activity against investigated tumor cells. In addition, an evident immunomodulatory effect c-EPS fraction was observed. Our show...

The effect of supplementation medium with rapeseed meal (RM) on production biotechnologically important enzymes was investigated in submerged cultures the white rot fungus Cerrena unicolor. addition RM (3.5% w/v) distinctly stimulated activities laccase, chitinase, and β-glucosidase. As compared to control, β-glucosidase, laccase supplemented were up 4.1, 8.4, 3.9 times higher, respectively. results spectrophotometric spectrofluorometric measurements additionally confirmed by zymographic...

Recent years have witnessed a fall in the numbers of bee colonies worldwide. To large extent, this is due to diseases affecting honeybees. One such disease nosemosis, which caused by fungi from genus Nosema. The antibiotic fumagallin used be administered treat but as it was found capable being transferred products, practice has ceased. We therefore focused on natural substances for treating disease, among them, aqueous extracts nest carton produced jet-black ant (Lasius fuliginosus). tested...

The present study examined Polish strains of Flamulina velutipes as a potential source nutraceuticals and found that their nutritional value is dependent on the fruiting bodies gathering time. To prove above hypothesis protein, carbohydrate phenolic substances concentration were determined. Moreover, catalase, superoxide dismutase, cellobiose dehydrogenase activities assayed. In order to healing properties Enoki obtained extracts tested for antioxidant bacteriostatic abilities. We have...

In our previous report, we had shown that the free-living amoeba Acanthamoeba castellanii influenced abundance, competiveness, and virulence of Mesorhizobium loti NZP2213, microsymbiont agriculturally important plants genus Lotus. The molecular basis this phenomenon; however, not been explored. present study, demonstrated oatB, O-acetyltransferase encoding gene located in lipopolysaccharide (LPS) synthesis cluster M. loti, was responsible for maintaining protective capacity bacterial cell...

According to epidemiological data, ovarian cancer is diagnosed in 240,000 women per year, which why it the seventh most common among worldwide. In addition, gynaecological cancers characterized by highest mortality. However, there still no effective treatment, maintenance therapies have a decisive impact on quality of life patients. Cerrena unicolor an example medical fungus that produces laccase enzyme (LAC), as well synthesizing low-molecular secondary metabolites with wide spectrum...

Background/Aim: Tumorigenesis and cancer progression might be driven by abnormal activation of growth factor receptors. Importantly, molecular changes in EGFR-dependent signaling is one the most common characteristics brain tumors. Patients Methods: HER1 EGFRvIII variants meningiomas glioblastomas were evaluated at RNA level. Results: was found 18.6% (GBM), whereas 25% positive tumors express wild-type EGFR as well. over-expressed benign compared to glioblastomas, expression differed...

Curdlan (β-1,3-glucan), as a biodegradable polymer, is still an underestimated but potentially attractive matrix for the production of dressing materials. However, due to its lack susceptibility functionalization, use limited. The proposed curdlan modification, using functional polycatecholamine layer, enables immobilization selected oxidoreductases (laccase and peroxidase) on hydrogel. following significant changes biological mechanical properties polycatecholamines +...