A5041912874- Acute Kidney Injury Research
- PARP inhibition in cancer therapy
- Ion Channels and Receptors
- Acute Lymphoblastic Leukemia research
- Sodium Intake and Health
- Renal and Vascular Pathologies
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Connective Tissue Growth Factor Research
- Toxin Mechanisms and Immunotoxins
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Mesenchymal stem cell research
- T-cell and Retrovirus Studies
- Dialysis and Renal Disease Management
- Climate Change and Health Impacts
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Psoriasis: Treatment and Pathogenesis
- Electrolyte and hormonal disorders
- Tissue Engineering and Regenerative Medicine
- IL-33, ST2, and ILC Pathways
- Diabetic Foot Ulcer Assessment and Management
- Advanced Glycation End Products research
- Renin-Angiotensin System Studies
Indiana University – Purdue University Indianapolis
2010-2021
Indiana University School of Medicine
2010-2021
Indiana University Health
2016-2021
University of Mississippi Medical Center
2020
Laboratoire d’immunologie intégrative du cancer
2017
Tata Institute of Fundamental Research
2008
A subset of poly ADP-ribose polymerases (PARP) that also contain macro domains regulate transcription. One such PARP, PARP-14 alters interleukin 4 (IL-4) and Stat6-dependent Stat6, activated by IL-4 plays an important role in T helper cell immunity B responses. Here we define the mechanism which regulates Stat6-activated Under non-stimulating conditions, recruits HDAC 2 3 to responsive promoters. In presence IL-4, promotes efficient binding Stat6 its target genes. Moreover, are released from...
T cells have been implicated in the pathogenesis of acute kidney injury (AKI) and its progression to chronic disease (CKD). Previous studies suggest that Th17 participate during AKI-to-CKD transition, inhibition cell activity by mycophenolate mofetil (MMF) or losartan attenuates development fibrosis following AKI. We hypothesized cell-deficient rats may reduced levels IL-17 cytokine leading decreased Renal ischemis-reperfusion (I/R) was performed on athymic (Foxn1rnu-/rnu-) control euthymic...
Reductions in renal microvasculature density and increased lymphocyte activity may play critical roles the progression of chronic kidney disease (CKD) following acute injury (AKI) induced by ischemia/reperfusion (IRI). Vitamin D deficiency is associated with tubulointerstitial damage fibrosis IRI-AKI. We evaluated effect vitamin sustained IRI-AKI, hypothesizing that such contributes to early reduction capillary or alters response IRI. Wistar rats were fed D-free standard diets for 35 days....
Damage to endothelial cells contributes acute kidney injury (AKI) by leading impaired perfusion. Endothelial colony-forming (ECFC) are precursor with high proliferative capacity, pro-angiogenic activity, and in vivo vessel forming potential. We hypothesized that ECFC may ameliorate the degree of AKI and/or promote repair renal vasculature following ischemia-reperfusion (I/R). Rat pulmonary microvascular (PMVEC) potential were compared artery (PAEC) low rats subjected I/R. PMVEC...
We hypothesized that the store-operated calcium entry (SOCE) channel, Orai1, participates in activation of Th17 cells and influences renal injury. In rats, following ischemia/reperfusion (I/R), there was a rapid sustained influx Orai1+ CD4 T IL-17 expression restricted to cells. When kidney CD4+ post-acute injury (post-AKI) rats were stimulated with angiotensin II elevated Na+ (10-7 M/170 mM) vitro, an enhanced response intracellular Ca2+ expression, which blocked by SOCE inhibitors 2APB,...
Highly aerobic organs like the kidney are innately susceptible to ischemia-reperfusion (I/R) injury, which can originate from sources including myocardial infarction, renal trauma, and transplant. Therapy is mainly supportive depends on cause(s) of damage. In absence hypervolemia, intravenous fluid delivery frequently first course treatment but does not reverse established AKI. Evidence suggests that disrupting leukocyte adhesion may prevent impairment microvascular perfusion heightened...
Abstract Timed degradation of the cyclin-dependent kinase inhibitor p27 Kip1 by E3 ubiquitin ligase F-box protein SKP2 is critical for T-cell progression into cell cycle, coordinating proliferation and differentiation processes. expression regulated mitogenic stimuli Notch signaling, a key pathway in development acute lymphoblastic leukemia (T-ALL); however, it not known whether plays role T-ALL. Here, we determined that function relevant T-ALL leukemogenesis, whereas dispensable...
Both acute and chronic kidney disease have a strong underlying inflammatory component. This review focuses primarily on T helper 17 (Th17) cells as mediators of inflammation their potential to modulate disease. We provide updated information factors signaling pathways that promote Th17 cell differentiation with specific reference highlight numerous clinical studies investigated in the setting human summaries from various experimental animal models indicating an important role for renal...
PARP-14, a member of the poly ADP-ribose polymerase super family, promotes T helper cell 2 (Th2) differentiation by regulating interleukin-4 (IL-4) and STAT6-dependent transcription. Yet, whether PARP-14 globally impacts gene regulation has not been determined. In this report, using an RNA pol II ChIP-seq approach, we identify genes in Th2 cells that are regulated either dependent or independent ADP-ribosyltransferase catalytic activity. Our data demonstrate enhances expression as it...
Acute kidney injury (AKI) is associated with high mortality rates and predisposes development of chronic disease (CKD). Distant organ damage, particularly in the lung, may contribute to AKI patients. Animal models demonstrate an increase pulmonary infiltration lymphocytes reveal acute compromise lung function, but effects on inflammation are unknown. We hypothesized that response renal ischemia/reperfusion (I/R), there a persistent systemic Th17 cells potential structure function. Renal I/R...
Abstract Damage to endothelial cells contributes acute kidney injury (AKI) by causing impaired perfusion, while the permanent loss of capillary network following AKI has been suggested promote chronic disease. Therefore, strategies protect renal vasculature may impact both short‐term recovery and long‐term functional preservation post‐AKI. Human adipose stromal (hASCs) possess pro‐angiogenic anti‐inflammatory properties therefore have tested as a therapeutic agent treat ischaemic conditions....
Transcription factors are critical determinants of T helper cell fate and require a variety co-factors to activate gene expression. We previously identified the ADP ribosyl-transferase poly-ADP-ribosyl polymerase 14 (PARP-14) as co-factor signal transducer activator transcription (STAT) 6 that is important in B-cell T-cell responses interleukin-4, particularly differentiation type 2 (Th2) cells. However, whether PARP-14 functions during development other subsets not known. In this report we...
Antidepressants induce structural remodeling in the adult hippocampus, including changes dendritic arbors, axonal sprouting, neurogenesis, and endothelial cell proliferation. Such forms of plasticity take place context extracellular matrix environment are known to be regulated by metalloproteinases (MMPs), particular MMP-2/9, their endogenous regulators, tissue inhibitors (TIMPs 1-4). Given hippocampal associated with antidepressant treatments, we hypothesized that antidepressants may...
Multiple clinical and preclinical studies have demonstrated that plasma levels of asymmetric dimethylarginine (ADMA) are strongly associated with hypertension, diabetes, cardiovascular renal disease. Genetic in rodents provided evidence ADMA metabolizing dimethylaminohydrolase (DDAH)-1 plays a role hypertension However, it remains to be established whether is bystander, biomarker, or sufficient contributor the pathogenesis The goal present investigation was develop pharmacological molecule...
Abstract Calcipotriene is a topical vitamin D 3 analogue approved for the treatment of plaque and scalp psoriasis. We report case 2‐year‐old boy whose atopic dermatitis ( AD ) flared in response to application calcipotriene 0.005% cream solution mistaken diagnosis investigated whether patient's eruption was secondary an allergic contact dermatitis. In Stat6 VT mouse model we tested could induce otherwise‐spontaneous ‐like phenotype. Closed patch testing done on patient with cream,...
To investigate T helper type 17 (Th17) cells in the setting of acute kidney injury, gene encoding master regulator Th17 cell differentiation, that is, RAR-related orphan receptor-γ (RORγT), was mutated Lewis rats using CRISPR/Cas9 technology. In response to 40 min bilateral renal ischemia-reperfusion (I/R), receptor C (Rorc)-/- were resistant injury relative wild-type Rorc+/+ rats. This protection associated with inhibition IL-17 expression and reduced infiltration CD4+ cells, CD8+ B...
A case of chronic neutrophilic leukaemia with a 4-year survival is reported. The platelet function abnormal due to deficiency storage pool adenine nucleotides. Neutrophils have paucity azurophil and specific granules. Circulating bone marrow colony-forming cells are markedly reduced.