A5050305033- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- Rabies epidemiology and control
- Viral Infections and Outbreaks Research
- Influenza Virus Research Studies
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Poxvirus research and outbreaks
- Lipid Membrane Structure and Behavior
- Microbial infections and disease research
- Viral Infectious Diseases and Gene Expression in Insects
- Venomous Animal Envenomation and Studies
- interferon and immune responses
- Computational Drug Discovery Methods
- Blood properties and coagulation
Anhui Agricultural University
2024-2025
First Affiliated Hospital of GuangXi Medical University
2025
Guangxi Medical University
2025
Harbin Veterinary Research Institute
2015-2024
Chinese Academy of Agricultural Sciences
2015-2024
Wuhan Institute of Technology
2024
Xiamen University of Technology
2023
China University of Petroleum, Beijing
2022
Emory University
2008-2020
First Affiliated Hospital of Chinese PLA General Hospital
1998-2014
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) causes the infectious disease COVID-19 (coronavirus 2019), which was first reported in Wuhan, China, December 2019. Despite extensive efforts to control disease, has now spread more than 100 countries and caused a global pandemic. SARS-CoV-2 is thought have originated bats; however, intermediate animal sources of virus are unknown. In this study, we investigated susceptibility ferrets animals close contact with humans SARS-CoV-2....
Abstract The unprecedented coronavirus disease 2019 (COVID-19) epidemic has created a worldwide public health emergency, and there is an urgent need to develop effective vaccine control this severe infectious disease. Here, we find that single vaccination with replication-defective human type 5 adenovirus encoding the SARS-CoV-2 spike protein (Ad5-nCoV) protect mice completely against mouse-adapted infection in upper lower respiratory tracts. Additionally, Ad5-nCoV protects ferrets from...
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important target for vaccine and drug development. However, the rapid emergence variant strains with mutated S proteins has rendered many treatments ineffective. Cleavage by host proteases essential viral infection. Here, we discovered that contains two previously unidentified Cathepsin L (CTSL) cleavage sites (CS-1 CS-2). Both are highly conserved among all known SARS-CoV-2 variants. Our structural...
The 3C-like protease (3CLpro) is an essential enzyme for the replication of SARS-CoV-2 and other coronaviruses thus a target coronavirus drug discovery. Nearly all inhibitors 3CLpro reported so far are covalent inhibitors. Here, we report development specific, noncovalent 3CLpro. most potent one, WU-04, effectively blocks replications in human cells with EC50 values 10-nM range. WU-04 also inhibits SARS-CoV MERS-CoV high potency, indicating that it pan-inhibitor showed anti-SARS-CoV-2...
H5N1 highly pathogenic avian influenza virus (HPAIV) has continued to spread and poses a significant threat both animal human health. Current vaccine strategies have limitations that prevent their effective use for widespread inoculation of animals in the field. Vaccine strains Newcastle disease (NDV), however, been used successfully easily vaccinate large numbers animals. In this study, we reverse genetics construct NDV expressed an H5 subtype (AIV) hemagglutinin (HA). Both wild-type...
Influenza prophylaxis would benefit from a simple method to administer influenza vaccine into skin without the need for hypodermic needles. In this study, solid metal microneedle arrays (MNs) were investigated as system cutaneous delivery using virus antigen. The MNs with 5 monument-shaped microneedles per array produced and coated inactivated A/PR/8/34 (IIV). As much 10 μg of viral proteins could be onto an microneedles, IIV was delivered at high efficiency within minutes. used immunize...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the infectious disease COVID-19, which was first reported in Wuhan, China December, 2019. Despite tremendous efforts to control disease, COVID-19 has now spread over 100 countries and caused a global pandemic. SARS-CoV-2 is thought have originated bats; however, intermediate animal sources of virus are completely unknown. Here, we investigated susceptibility ferrets animals close contact with humans SARS-CoV-2. We...
Rabies virus (RABV) invades the central nervous system and nearly always causes fatal disease in humans. How RABV interacts with host neuron membrane receptors to become internalized cause rabid symptoms is not yet fully understood. Here, we identified a novel receptor of RABV, which uses infect neurons. We found that metabotropic glutamate subtype 2 (mGluR2), member G protein-coupled family abundant system, directly glycoprotein mediate entry. infection was drastically decreased after...
Minks are raised in many countries and have transmitted severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to humans. However, the biologic properties of SARS-CoV-2 minks largely unknown. Here, we investigated found that replicates efficiently both upper lower tracts, transmits via droplets; pulmonary lesions caused by similar those seen humans with COVID-19. We further a spike protein-based subunit vaccine prevented replication lung damage infection minks. Our study indicates...
Abstract SARS-CoV-2 and filovirus enter cells via the cell surface angiotensin-converting enzyme 2 (ACE2) or late-endosome Niemann-Pick C1 (NPC1) as a receptor. Here, we screened 974 natural compounds identified Tubeimosides I, II, III pan-coronavirus entry inhibitors that target NPC1. Using in-silico, biochemical, genomic approaches, provide evidence NPC1 also binds spike (S) protein on receptor-binding domain (RBD), which is blocked by Tubeimosides. Importantly, strongly promotes...
ABSTRACT Effective, safe, and affordable rabies vaccines are still being sought. Newcastle disease virus (NDV), an avian paramyxovirus, has shown promise as a vaccine vector for mammals. Here, we generated recombinant avirulent NDV La Sota strain expressing the glycoprotein (RVG) evaluated its potential to serve against rabies. The virus, rL-RVG, retained high-growth property in chicken eggs, with titers of up 10 9.8 50% egg infective doses (EID 50 )/ml allantoic fluid. RVG expression...
The unprecedented coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), is a serious threat to global public health. Development of effective therapies against SARS-CoV-2 urgently needed. Here, we evaluated the antiviral activity remdesivir parent nucleotide analog, GS441524, which targets RNA-dependent RNA polymerase enzyme, and feline prodrug, GC376, its main protease, using mouse-adapted infected mouse model. Our results showed that...
The immune response induced by immunization with HIV Env DNA and virus-like particle (VLP) vaccines was investigated. Immunization the vaccine a strong CD8 T cell but relatively weak antibody against whereas VLPs higher levels of responses little response. Interestingly, mixture VLP enhanced responses. Further, it observed that mixing during is necessary for augmenting induction such augmentation also control VLPs. These results show combines advantages both platforms eliciting high
Abstract Outbreaks of coronaviruses (CoVs), especially severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), have posed serious threats to humans and animals, which urgently calls for effective broad‐spectrum antivirals. RNA‐dependent RNA polymerase (RdRp) plays an essential role in viral synthesis is ideal pan‐coronaviral therapeutic target. Herein, based on cryo‐electron microscopy biochemical approaches, gossypol (GOS) identified from 881 natural products directly block SARS‐CoV‐2...
Rabies virus (RABV) invades the central nervous system and nearly always causes fatal disease in humans. RABV enters cells via clathrin-mediated endocytosis upon receptor binding. The detailed mechanism of this process how it is regulated are not fully understood. Here, we carried out a high-through-put RNAi analysis identified AP2-associated kinase 1 (AAK1), serine/threonine kinase, as an important cellular component regulating entry RABV. AAK1 knock-down greatly inhibits infection cells,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses angiotensin-converting enzyme (ACE2) as a binding receptor to enter cells via clathrin-mediated endocytosis (CME). However, receptors involved in other steps of SARS-CoV-2 infection remain largely unknown. Here, we found that metabotropic glutamate subtype (mGluR2) is an internalization factor for SARS-CoV-2. Our results show mGluR2 directly interacts with the spike protein and knockdown decreases but not cell binding....
The continuous emergence of severe acute respiratory coronavirus 2 (SARS-CoV-2) variants and the increasing number breakthrough infection cases among vaccinated people support urgent need for research development antiviral drugs. Viral entry is an intriguing target drug development. We found that diltiazem, a blocker L-type calcium channel Cav1.2 pore-forming subunit (Cav1.2 α1c) FDA-approved drug, inhibits binding internalization SARS-CoV-2, decreases SARS-CoV-2 in cells mouse lung. α1c...
For most viruses, cell entry involves engagement with many distinct plasma membrane components, each of which is essential. After binding to its specific receptor(s), rabies virus (RABV) enters host cells through the process clathrin-mediated endocytosis.
Several conserved neutralizing epitopes have been identified in the HIV Env protein and among these, MPER of gp41 has received great attention is widely recognized as a promising target. However, little success achieved eliciting MPER-specific antibodies by number different vaccine strategies. We investigated ability HA/gp41 chimeric protein-based vaccines, which were designed to enhance exposure its native conformation, induce antibodies. In characterization protein, we found that mutating...