A5077067683- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- interferon and immune responses
- Virus-based gene therapy research
- Immune Response and Inflammation
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
Harbin Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Gansu Agricultural University
2012-2024
Ministry of Agriculture and Rural Affairs
2024
China Agricultural University
2024
Yangzhou University
2021-2024
Key Laboratory of Guangdong Province
2021-2023
State Key Laboratory of Veterinary Biotechnology
2021
Ministère de l'Agriculture et de la Souveraineté alimentaire
2005-2016
Animal Science Research Institute
2012
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) causes the infectious disease COVID-19 (coronavirus 2019), which was first reported in Wuhan, China, December 2019. Despite extensive efforts to control disease, has now spread more than 100 countries and caused a global pandemic. SARS-CoV-2 is thought have originated bats; however, intermediate animal sources of virus are unknown. In this study, we investigated susceptibility ferrets animals close contact with humans SARS-CoV-2....
ABSTRACT We recently analyzed a series of H5N1 viruses isolated from healthy ducks in southern China since 1999 and found that these had progressively acquired the ability to replicate cause disease mice. In present study, we explored genetic basis this change host range by comparing two are genetically similar but differ their infect mice have different pathogenicity A/duck/Guangxi/22/2001 (DKGX/22) is nonpathogenic mice, whereas A/duck/Guangxi/35/2001 (DKGX/35) highly pathogenic. used...
ABSTRACT In this study, we explored the molecular basis determining virulence of H5N1 avian influenza viruses in mammalian hosts by comparing two viruses, A/Duck/Guangxi/12/03 (DK/12) and A/Duck/Guangxi/27/03 (DK/27), which are genetically similar but differ their pathogenicities mice. To assess genetic for difference virulence, used reverse genetics to generate a series reassortants mutants these viruses. We found that single-amino-acid substitution serine proline at position 42 (P42S) NS1...
Influenza A viruses (IAVs), particularly H1N1, H5N1 and H7N9, pose a substantial threat to public health worldwide. Here, we report that MIR2911, honeysuckle (HS)-encoded atypical microRNA, directly targets IAVs with broad spectrum. MIR2911 is highly stable in HS decoction, continuous drinking or gavage feeding of decoction leads significant elevation the level mouse peripheral blood lung. Bioinformatics prediction luciferase reporter assay showed could target various IAVs, including H7N9....
A newly emerged H7N9 virus has caused 132 human infections with 37 deaths in China since 18 February 2013. Control measures virus-positive live poultry markets have reduced the number of infections; however, character virus, including its pandemic potential, remains largely unknown. We systematically analyzed viruses isolated from birds and humans. The were genetically closely related bound to airway receptors; some also maintained ability bind avian receptors. nonpathogenic chickens, ducks,...
Since 2003, H5N1 influenza viruses have caused over 400 known cases of human infection with a mortality rate greater than 60%. Most these resulted from direct contact virus-contaminated poultry or products. Although only limited human-to-human transmission has been reported to date, it is feared that efficient the potential cause pandemic disastrous proportions. The genetic basis for viral among humans largely unknown. In this study, we used guinea pigs as mammalian model study six different...
H5N1 influenza A viruses are widely distributed among poultry in Asia, but until recently, only a limited number of wild birds were affected. During late April through June 2005, an outbreak virus infection occurred at Qinghai Lake China. Here, we describe the features this outbreak. First identified bar-headed geese, disease soon spread to other avian species populating lake. Sequence analysis 15 representing six and collected different times during revealed four genotypes. Most isolates...
Avian influenza A H5N1 viruses continue to spread globally among birds, resulting in occasional transmission of virus from infected poultry humans. Probable human-to-human has been documented rarely, but have not yet acquired the ability transmit efficiently humans, an essential property a pandemic virus. The pandemics 1957 and 1968 were caused by avian-human reassortant that had human virus-like receptor binding properties. However, relative contribution internal protein genes or other...
Ning Li and colleagues report the whole-genome sequence of duck, Anas platyrhynchos, a natural host avian influenza viruses. They examine response to infection by comparing lung transcriptomes ducks that were infected with A The duck (Anas platyrhynchos) is one principal hosts We present genome perform deep transcriptome analyses investigate immune-related genes. Our data indicate possesses contractive immune gene repertoire, as in chicken zebra finch, this repertoire has been shaped through...
H9N2 subtype influenza viruses have been detected in different species of wild birds and domestic poultry many countries for several decades. Because these are low pathogenicity poultry, their eradication is not a priority animal disease control countries, which has allowed them to continue evolve spread. Here, we characterized the genetic variation, receptor-binding specificity, replication capability, transmission mammals series that were live markets southern China between 2009 2013....
In the past, avian influenza viruses have crossed species barriers to trigger human pandemics by reassorting with mammal-infective in intermediate livestock hosts. H5N1 are able infect pigs, and some of them affinity for mammalian type α-2,6-linked sialic acid airway receptor. Using reverse genetics, we systematically created 127 reassortant between a duck isolate H5N1, specifically retaining its hemagglutinin (HA) gene throughout, highly transmissible, human-infective H1N1 virus. We tested...
Certain low pathogenic avian influenza viruses can mutate to highly when they circulate in domestic poultry, at which point cause devastating poultry diseases and severe economic damage. The H7N9 that emerged 2013 China had caused human infections deaths. However, these were nonlethal poultry. It is unknown whether the acquire additional mutations during their circulation nature become lethal more dangerous for humans. Here, we evaluated evolution of isolated from species between 2017 found...
H5N1 avian influenza viruses bearing the clade 2.3.4.4b hemagglutinin gene have been widely circulating in wild birds and are responsible for loss of over 70 million domestic poultry Europe, Africa, Asia, North America since October 2020. During our routine surveillance, 13 were isolated from 26,767 bird samples that collected between September 2021 March 2022 China. To investigate origin these Chinese isolates understand their genetic relationship with globally viruses, we performed a...
The globally circulating H5N8 avian influenza viruses bearing the clade 2.3.4.4b hemagglutinin (HA) gene are responsible for loss of more than 33 million domestic poultry since January 2020. Moreover, have reassorted with other and formed H5N1, H5N2, H5N3, H5N4, H5N5 in Europe, Africa, North America. In this study, we analyzed 15 H5N6 isolated from seven humans, found these different genotypes by deriving HA viruses, neuraminidase duck internal genes that previously circulated ducks wild...
Highly pathogenic avian influenza (HPAI) subtype H5N1 clade 2.3.4.4b virus has spread globally, causing unprecedented large-scale outbreaks since 2020. In 2021, we isolated 17 highly viruses from wild birds in China. To determine origin, genetically analyzed 1,529 reported globally October 2020 and found that they formed 35 genotypes. The belonged to genotypes G07, which originated eastern Asia, G10, Russia. were moderately mice but lethal ducks. the same antigenic cluster as current vaccine...
Recently, an outbreak of highly pathogenic avian influenza A (H5N1), which carries the clade 2.3.4.4b hemagglutinin (HA) gene and has been prevalent among North American bird populations since winter 2021, was reported in dairy cows United States. As May 24, 2024, virus affected 63 herds across nine states resulted two human infections. The causes unusual symptoms cows, including unexpected drop milk production, thick colostrum-like milk. Notably, US Food Drug Administration that around 20%...
Influenza virus infection is initiated by the attachment of viral haemagglutinin (HA) protein to sialic acid receptors on host cell surface. Most particles enter cells through clathrin-mediated endocytosis (CME). However, it unclear how binding signals are transmitted plasma membrane triggering CME. Here we found that metabotropic glutamate receptor subtype 2 (mGluR2) and potassium calcium-activated channel subfamily M alpha 1 (KCa1.1) involved in initiation completion CME influenza using an...
ABSTRACT In the present study, we explored genetic basis underlying virulence and host range of two H5N1 influenza viruses in chickens. A/goose/Guangdong/1/96 (GS/GD/1/96) is a highly pathogenic virus for chickens, whereas A/goose/Guangdong/2/96 (GS/GD/2/96) unable to replicate These differ sequence by only five amino acids mapping PA, NP, M1, NS1 genes. We used reverse genetics create four single-gene recombinants that contained one sequence-differing genes from nonpathogenic GS/GD/2/96...
H5N1 highly pathogenic avian influenza virus (HPAIV) has continued to spread and poses a significant threat both animal human health. Current vaccine strategies have limitations that prevent their effective use for widespread inoculation of animals in the field. Vaccine strains Newcastle disease (NDV), however, been used successfully easily vaccinate large numbers animals. In this study, we reverse genetics construct NDV expressed an H5 subtype (AIV) hemagglutinin (HA). Both wild-type...