A5039519721- HIV Research and Treatment
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Animal Virus Infections Studies
- Signaling Pathways in Disease
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- HIV-related health complications and treatments
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Iron Metabolism and Disorders
- Kawasaki Disease and Coronary Complications
- Respiratory viral infections research
- Peripheral Neuropathies and Disorders
- Autoimmune and Inflammatory Disorders Research
- Immunodeficiency and Autoimmune Disorders
- Hemoglobinopathies and Related Disorders
- Pharmacological Effects and Toxicity Studies
- Vaccine Coverage and Hesitancy
- Mosquito-borne diseases and control
- interferon and immune responses
Instituto de Biomedicina de Sevilla
2015-2024
Hospital Universitario Virgen del Rocío
2016-2024
Universidad de Sevilla
2016-2024
Albert Einstein College of Medicine
2018-2024
Influenza vaccine effectiveness is not optimal in solid organ transplant recipients (SOTR). We hypothesized that a booster dose might increase it.TRANSGRIPE 1-2 phase 3, randomized, controlled, multicenter, open-label clinical trial. Patients were randomly assigned (1:1 stratified by study site, type of organ, and time since transplantation) to receive 1 (control group) or 2 doses (booster the influenza 5 weeks apart.A total 499 SOTR enrolled. Although seroconversion at 10 did meet...
Disruption of cyclophilin A (CypA)-capsid interactions affects HIV-1 replication in human lymphocytes. To understand this mechanism, we utilize Jurkat cells, peripheral blood mononuclear cells (PBMCs), and CD4+ T cells. Our results show that inhibition infection caused by disrupting CypA-capsid is dependent on tripartite motif 5α (TRIM5αhu), showing TRIM5αhu restricts Accordingly, depletion rescues fail to interact with CypA, such as HIV-1-P90A. We found binds the core. affect...
Abstract SAMHD1 impedes infection of myeloid cells and resting T lymphocytes by retroviruses, the enzymatic activity protein—dephosphorylation deoxynucleotide triphosphates (dNTPs)—implicates dNTP depletion in innate antiviral immunity. Here we show that allosteric binding sites enzyme are plastic can accommodate oligonucleotides place activators, GTP dNTP. displays a preference for containing phosphorothioate bonds Rp configuration located 3’ to G nucleotides (GpsN), modification pattern...
Aging | doi:10.18632/aging.203109. Vanesa Garrido-RodrÃguez, Inés Herrero-Fernández, MarÃa José Castro, Ana Castillo, Isaac Rosado-Sánchez, Isabel Galvá, Raquel Ramos, Israel Olivas-MartÃnez, Ãngel Bulnes-Ramos, Julio Cañizares, Manuel Leal, Yolanda Pacheco
Our goal was to study whether influenza vaccination induced antibody mediated rejection in a large cohort of solid organ transplant recipients (SOTR).Serum anti-Human Leukocyte Antigen (HLA) antibodies were determined using class I and II antibody-coated latex beads (FlowPRATM Screening Test) by flow cytometry. Anti-HLA specificity the single-antigen bead cytometry (SAFC) assay assignation donor specific (DSA) performed virtual-crossmatch.We studied 490 SOTR that received an from 2009 2013:...
Seasonal influenza virus vaccination should be considered in all pediatric patients with rheumatic diseases. Few studies have addressed safety and efficacy this group. We aim to prospectively evaluate immunogenicity of the trivalent inactivated vaccine including A/H1N1, A/H3N2 B strains children juvenile idiopathic arthritis (JIA) receiving biological therapy. Thirty-five diagnosed JIA 6 healthy siblings were included. Serum samples collected prior to, 4-8 weeks one year after vaccination....
Iron metabolism plays an essential role in cellular functions. Since virologically suppressed chronic HIV-infected subjects under effective antiretroviral treatment (ART) exhibit a persistent immune dysfunction that leads to comorbidities, iron homeostasis may be relevant this context. We aimed explore HIV infected successful ART.In retrospective study, traditional biomarkers (total iron, ferritin, transferrin, and transferrin saturation index), as well soluble receptor (sTfR), hepcidin,...
The core of HIV-1 viruses bearing the capsid change N74D (HIV-1-N74D) do not bind human protein CPSF6. In primary CD4+ T cells, HIV-1-N74D exhibit an infectivity defect when compared to wild-type. We first investigated whether loss CPSF6 binding accounts for infectivity. Depletion in cells did affect early stages wild-type replication, suggesting that defective case is due binding. Based on our previous result cyclophilin A (Cyp A) protected from tripartite motif-containing 5α (TRIM5αhu)...
Older people achieve lower levels of antibody titers than younger populations after Covid-19 vaccination and show a marked waning humoral immunity over time, likely due to the senescence immune system. Nevertheless, age-related predictive factors response vaccine have been scarcely explored. In cohort residents healthcare workers from nursing home that had received two doses BNT162b2 vaccine, we measured specific anti-S antibodies one (T1), four (T4), eight (T8) months receiving second dose....
ABSTRACT In humans, sterile alpha motif (SAM) domain- and histidine–aspartic acid (HD) domain-containing protein 1 (SAMHD1) is a dNTPase enzyme that prevents HIV-1 infection in non-cycling cells, such as differentiated THP-1 cells human primary macrophages. Although phosphorylation of threonine 592 (T592) SAMHD1 recognized the regulator ability to prevent infection, contributions acetylation this remain unknown. Mass spectrometry analysis proteins derived from cycling B macrophages revealed...
Introduction Kidney transplant recipients showed a weak humoral response to the mRNA COVID-19 vaccine despite receiving three cumulative doses of vaccine. New approaches are still needed raise protective immunity conferred by administration within this group high-risk patients. Methods To analyze and identify any predictive factors these patients, we designed prospective monocentric longitudinal study (KTR) who received mRNA-1273 Specific antibody levels were measured chemiluminescence....
Persistence of a low CD4/CD8 ratio is associated with an increased morbimortality in people living HIV (PLWH) under effective antiretroviral therapy. We aimed to explore the immunological significance persistently ratio, even despite normal CD4 levels, and assess whether these features vary from those nadir-CD4, another well-established predictor disease progression.
ABSTRACT Disruption of cyclophilin A (CypA)-capsid interactions affects HIV-1 replication in human lymphocytes. To understand the mechanism, we used Jurkat cells, PBMCs, and CD4 + T cells. Our results showed that inhibition infection caused by disrupting CypA-capsid is dependent on TRIM5α (TRIM5α hu ), suggesting restricts HIV-1. Accordingly, found binds to core. failed affect HIV-1-A92E infection, correlating with loss binding cores. PBMCs cells had a greater inhibitory effect when compared...
ABSTRACT The core of HIV-1 viruses bearing the capsid change N74D (HIV-1-N74D) do not bind human protein cleavage and polyadenylation specificity factor subunit 6 (CPSF6). In addition, HIV-1-N74D have altered patterns integration site preference in cell lines. primary CD4+ T cells, exhibit infectivity defects when compared to wild type. reason for this loss cells is unknown. We first investigated whether CPSF6 binding accounts infectivity. Depletion CD4 + did affect early stages wild-type...