A5076035732- interferon and immune responses
- Aquaculture disease management and microbiota
- HIV Research and Treatment
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Circadian rhythm and melatonin
- Viral Infections and Vectors
- Antimicrobial Peptides and Activities
- Inflammasome and immune disorders
- Systemic Lupus Erythematosus Research
- COVID-19 Clinical Research Studies
- Angiogenesis and VEGF in Cancer
- Protein Hydrolysis and Bioactive Peptides
- Multiple Sclerosis Research Studies
- Autophagy in Disease and Therapy
- Zebrafish Biomedical Research Applications
- Phagocytosis and Immune Regulation
- Animal Virus Infections Studies
- Immune cells in cancer
- HIV/AIDS drug development and treatment
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Virus-based gene therapy research
Centre for Biomedical Network Research on Rare Diseases
2021-2025
Instituto Murciano de Investigación Biosanitaria
2021-2025
Instituto de Investigación de Enfermedades Raras
2021-2025
Instituto de Salud Carlos III
2021-2025
Universidad de Sevilla
2014-2022
Universidad de Murcia
2021-2022
Centro de Investigación Biomédica en Red
2022
Albert Einstein College of Medicine
2017-2021
Instituto de Biomedicina de Sevilla
2014-2021
Junta de Andalucía
2020-2021
Human immunodeficiency virus type 1 (HIV-1) displays the unique ability to infect nondividing cells. The capsid of HIV-1 is viral determinant for nuclear import. To understand cellular factors involved in cells, we sought find mutations that allow dividing but not Because interaction with nucleoporin protein 153 (Nup153) important import HIV-1, solved new crystal structures hexameric complex a Nup153-derived peptide containing phenylalanine-glycine repeat (FG repeat), which used guide...
Amaranthus caudatus L. (Amaranthaceae), commonly known as kiwicha, is considered one of the few multipurpose pseudocereal crops which supply higher nutritional seeds in huge quantities. A. rich source proteins, β-carotene, vitamins, minerals, and dietary fiber. Amaranth starch promising use by its high solubility digestibility, compared to wheat, rice, oat, amaranths are gluten-free contain 30% more protein with complete set amino acid, offering new possibilities for food processing,...
Disruption of cyclophilin A (CypA)-capsid interactions affects HIV-1 replication in human lymphocytes. To understand this mechanism, we utilize Jurkat cells, peripheral blood mononuclear cells (PBMCs), and CD4+ T cells. Our results show that inhibition infection caused by disrupting CypA-capsid is dependent on tripartite motif 5α (TRIM5αhu), showing TRIM5αhu restricts Accordingly, depletion rescues fail to interact with CypA, such as HIV-1-P90A. We found binds the core. affect...
Previous research has shown that an antimicrobial peptide (AMP) of the myticin class C (Myt C) is most abundantly expressed gene in cDNA and suppressive subtractive hybridization (SSH) libraries after immune stimulation mussel Mytilus galloprovincialis. However, to date, expression pattern, activities immunomodulatory properties Myt have not been determined. In contrast, it known mRNA presents unusual high level polymorphism unidentified biological significance. Therefore, provide a better...
It has not been elucidated whether or autophagy is induced by rhabdoviral G glycoproteins (G) in vertebrate organisms for which rhabdovirus infection lethal. Our work provides the first evidence that both mammalian (vesicular stomatitis virus, VSV) and fish (viral hemorrhagic septicemia VHSV, spring viremia carp SVCV) Gs induce an autophagic antiviral program cell lines. The transcriptomic profiles obtained from zebrafish genetically immunized with either Gsvcv Gvhsv suggest shortly after...
The coronavirus disease 2019 (COVID-19) pandemic turned the whole world upside down in a short time. One of main challenges faced has been to understand COVID-19–associated life-threatening hyperinflammation, so-called cytokine storm syndrome (CSS). We report here proinflammatory role Spike (S) proteins from different severe acute respiratory 2 (SARS-CoV-2) variants concern zebrafish. found that wild-type/Wuhan variant S1 (S1WT) promoted neutrophil and macrophage recruitment, local systemic...
Multiple sclerosis (MS) is a neuroinflammatory disease of the central nervous system in which immune plays role. In particular, effector populations such as T helper (Th) 1, Th9, Th17, and Th22 cells are involved development, whereas regulatory (Tregs) associated with resolution disease. Melatonin levels impaired patients MS, exogenous melatonin ameliorates MS animal models by modulating Th1/Th17/Treg responses also improves quality life several symptoms MS. However, no study has examined...
We describe the case of a female patient who, at age 28, was diagnosed with symptoms primary progressive multiple sclerosis (PPMS). Glucocorticoid treatment immediately initiated. The disease and demyelinating lesions progressed during following 9 years reaching Expanded Disability Status Scale (EDSS) 8.0 (patient essentially restricted to bed, chair or perambulated in wheelchair). At this point, began taking melatonin doses ranging from 50 300 mg per day. Melatonin her only for next 4...
Abstract SAMHD1 impedes infection of myeloid cells and resting T lymphocytes by retroviruses, the enzymatic activity protein—dephosphorylation deoxynucleotide triphosphates (dNTPs)—implicates dNTP depletion in innate antiviral immunity. Here we show that allosteric binding sites enzyme are plastic can accommodate oligonucleotides place activators, GTP dNTP. displays a preference for containing phosphorothioate bonds Rp configuration located 3’ to G nucleotides (GpsN), modification pattern...
We have previously demonstrated the anti-inflammatory and antioxidant properties of in vitro administered Lupinus angustifolius protein hydrolysates (LPHs) on human peripheral blood mononuclear cells (PBMCs). This study aims to evaluate safety efficacy a beverage containing LPHs (LPHb) immune, oxidative metabolic status healthy subjects.In this open-label intervention, 33 participants daily ingest LPHb 1 g for 28 days. Biochemical parameters are assayed fasting urine samples before, during...
Sea bass nervous necrosis virus is the causative agent of viral necrosis, a disease responsible high economic losses in larval and juvenile stages cultured sea (Dicentrarchus labrax). To identify genes potentially involved antiviral immune defense, gene expression profiles response to nodavirus infection were investigated head kidney using suppression subtractive hybridization (SSH) technique. A total 8.7% expressed sequence tags found SSH library showed significant similarities with genes,...
TNFα is a pleiotropic pro-inflammatory cytokine with key role in the activation of immune system to fight viral infections. Despite its antiviral role, few viruses might utilize host produced their benefit. Some recent reports have shown that anti-TNFα therapies could be utilized treat certain However, underlying mechanisms by which can favor virus replication not been identified. Here, rhabdoviral infection model zebrafish allowed us identify mechanism action Tnfa has deleterious for combat...
BackgroundFlaviviruses such as Zika cause sporadic pandemic outbreaks worldwide. There is an urgent need for anti-Zika virus (ZIKV) drugs to prevent mother-to-child transmission of ZIKV, new infections in high-risk populations, and the infection medical personnel ZIKV-affected areas.MethodsHere, we showed that small molecule 6-deoxyglucose-diphyllin (DGP) exhibited anti-ZIKV activity both vitro vivo. DGP potently blocked ZIKV across all human monkey cell lines tested. also displayed...
To investigate fish innate immunity, we have conducted organ and cell immune-related transcriptomic as well immunohistologic analysis in mutant zebra (Danio rerio) lacking adaptive immunity (rag1-/-) at different developmental stages (egg, larvae, adult), before after infection with spring viremia carp virus (SVCV). The results revealed that, compared to immunocompetent (rag1+/+ ), rag1-/- acquired increased resistance SVCV age, correlating elevated transcript levels of immune genes...
Abstract In mammals, recombination activating gene 1 (RAG1) plays a crucial role in adaptive immunity, generating vast range of immunoglobulins. Rag1 −/− zebrafish ( Danio rerio ) are viable and reach adulthood without obvious signs infectious disease standard nonsterile conditions, suggesting that innate immunity could be enhanced to compensate for the lack immunity. By using microarray analysis, we confirmed expression immunity‐ apoptosis‐related genes was increased rag1 fish. This tool...
One of the most studied defense mechanisms against invading pathogens, including viruses, are Toll-like receptors (TLRs). Among them, TLR3, TLR7, TLR8 and TLR9 detect different forms viral nucleic acids in endosomal compartments, whereas TLR2 TLR4 recognize structural nonstructural proteins outside cell. Although many TLRs have been shown to be involved SARS-CoV-2 infection detection proteins, studies performed vitro results obtained rather contradictory. In this study, we report using...
Surface glycoproteins of enveloped virus are potent elicitors type I interferon (IFN)-mediated antiviral responses in a way that may be independent the well-studied genome-mediated route. However, viral glycoprotein determinants responsible for initiating IFN response remain unidentified. In this study, we have used collection 60 synthetic 20-mer overlapping peptides (pepscan) spanning full length G (gpG) hemorrhagic septicemia (VHSV) to investigate what regions protein implicated triggering...