A5005808846- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Immune Cell Function and Interaction
- Wireless Networks and Protocols
- Viral Infectious Diseases and Gene Expression in Insects
- Nanowire Synthesis and Applications
- Innovation and Socioeconomic Development
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Phagocytosis and Immune Regulation
- Nanofabrication and Lithography Techniques
- Advanced biosensing and bioanalysis techniques
- Cooperative Communication and Network Coding
- IL-33, ST2, and ILC Pathways
- Advanced Wireless Network Optimization
- Genomics, phytochemicals, and oxidative stress
- Biosimilars and Bioanalytical Methods
- Endoplasmic Reticulum Stress and Disease
- 3D Printing in Biomedical Research
Korea Advanced Institute of Science and Technology
2016-2024
Myongji University
2024
New Generation University College
2024
Seoul National University
2024
Hanyang University
2024
Kangwon National University
2024
Daejeon University
2017
Government of the Republic of Korea
2017
Abstract CRISPR-Cas9 nucleases are versatile tools for genetic engineering cells and function by producing targeted double-strand breaks (DSBs) in the DNA sequence. However, unintended production of large deletions (>100 bp) represents a challenge to effective application this genome-editing system. We optimized long-range amplicon sequencing system developed k-mer sequence-alignment algorithm simultaneously detect small alteration events deletions. With workflow, we determined that...
Cytotoxic T lymphocytes (CTLs) play a crucial role in cancer rejection. However, CTLs encounter dysfunction and exhaustion the immunosuppressive tumor microenvironment (TME). Although reactive oxygen species (ROS)-rich TME attenuates CTL function, underlying molecular mechanism remains poorly understood. The nuclear factor erythroid 2-related 2 (Nrf2) is ROS-responsible implicated increasing susceptibility to progression. Therefore, we examined how Nrf2 involved anti-tumor responses of CD8
An immunoreaction for immunohistochemistry is enhanced by pipetting-driven bidirectional flows in a microfluidic device.
When used to edit genomes, Cas9 nucleases produce targeted double-strand breaks in DNA. Subsequent DNA-repair pathways can induce large genomic deletions (larger than 100 bp), which constrains the applicability of genome editing. Here we show that Cas9-mediated at varying frequencies cancer cell lines, human embryonic stem cells and primary T cells, most are produced by two repair pathways: end resection DNA-polymerase theta-mediated joining. These findings required optimization long-range...
Background The incorporation of co-stimulatory signaling domains into second-generation chimeric antigen receptors (CARs) significantly enhances the proliferation and persistence CAR-T cells in vivo, leading to successful clinical outcomes. Methods To achieve such functional enhancement transgenic T-cell receptor-engineered (TCR-T) therapy, we designed a TCR-T cell which CD3ζ genes modified contain intracellular domain (ICD) 4-1BB receptor were selectively inserted CD247 locus. Results This...
<title>Abstract</title> CRISPR-Cas9 nucleases are versatile tools for genetic engineering cells and function by producing targeted double-strand breaks (DSBs) in the DNA sequence. However, unintended production of large deletions (> 100 bp) represents a challenge to effective application this genome-editing system. We optimized long-range amplicon sequencing system developed k-mer sequence-alignment algorithm simultaneously detect small alteration events deletions. With workflow, we...
Other SectionsABSTRACTINTRODUCTIONGENOME EDITING WITH CRISPR/CAS9: HISTORY AND MECHANISMCRISPR/CAS9-BASED GENOME FOR THERAPEUTIC T-CELL ENGINEERINGCRISPR/CAS9-BASED IN OTHER IMMUNE-CELL TYPESCRISPR/CAS9-BASED GENOME-WIDE SCREENING IMMUNE CELLSCONCLUDING REMARKSCONFLICTS OF INTERESTFIGURESREFERENCES
We present here a novel microfluidic platform that can perform on-chip immunohistochemistry (IHC) processes on formalin-fixed paraffin-embedded section slide. Unlike previous IHC studies, our chip made of organic solvent-resistant polyurethane acrylate (PUA) is capable conducting consecutively. A narrow channel wall structure the PUA shows effective sealing by pressure-based reversible assembly with performed both and conventional compared results based immunostaining intensity. The result...
People are seeking solutions in diverse directions to cope with mobile data explosion and resource scarcity cellular networks. Of many candidate approaches, smart aggregation of LTE Wi-Fi radios is a promising solution that bonds heterogeneous links meet terminal's available bandwidth need. Motivated by the existence significant number carrier-operated APs, we propose mechanism, called LTE-W, efficiently utilizing only minimum changes eNodeBs, backhaul networks, terminals. Our mechanism has...
<h3>Background</h3> Interleukin-12 (IL-12) is a powerful immunostimulatory cytokine that has been expressed ectopically in genetically engineered T cells to enhance their antitumor activity. However, the constitutive production of IL-12 by caused severe adverse effects patients. Thus, fully harness potential while avoiding systemic toxicity, we inserted <i>IL-12</i> gene into <i>PDCD1</i> locus cell receptor (TCR)-engineered using CRISPR/Cas9-based genome editing tool, which allows for...