A5007510088- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Supramolecular Self-Assembly in Materials
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Vibrio bacteria research studies
- Crystallography and molecular interactions
- Biotin and Related Studies
- Cell Image Analysis Techniques
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- Synthetic Organic Chemistry Methods
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Endoplasmic Reticulum Stress and Disease
- Bacterial biofilms and quorum sensing
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Advanced Biosensing Techniques and Applications
- Tryptophan and brain disorders
- Metal complexes synthesis and properties
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Synthesis and Biological Evaluation
- Catalytic Cross-Coupling Reactions
Technical University of Munich
2017-2023
Institute of Organic Chemistry
2021
Center for Integrated Protein Science Munich
2017-2019
Georgia Institute of Technology
2018
Aqueous solutions of the achiral, monomeric, nucleobase mimics (2,4,6-triaminopyrimidine, TAP, and a cyanuric acid derivative, CyCo6) spontaneously assemble into macroscopic homochiral domains supramolecular polymers. These assemblies exhibit high degree chiral amplification. Addition small quantity one handedness derivative CyCo6 generates exclusively structures. This system exhibits highest reported amplification for dynamic helical polymers or helices. Significantly, comprised hexameric...
SignificanceHost-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes quantitative proteomics to identify target epinephrine α-adrenoceptor agonist phenylephrine in live cells aquatic pathogen Vibrio campbellii. Consequently, discovered coupling protein CheW, which is center chemotaxis signaling network, as a both molecules. We not only demonstrate...
We review how chemical proteomic tools have been used to elucidate biological processes at the interface of bacteria and eukaryotic hosts, which can be important in establishment pathogenicity.
Abstract Natural products represent a rich source of antibiotics that address versatile cellular targets. The deconvolution their targets via chemical proteomics is often challenged by the introduction large photocrosslinkers. Here we applied elegaphenone, largely uncharacterized natural product antibiotic bearing native benzophenone core scaffold, for affinity‐based protein profiling (AfBPP) in Gram‐positive and Gram‐negative bacteria. This study utilizes alkynylated scaffold as probe to...
Fluorescent Pd(<sc>ii</sc>) and Pt(<sc>ii</sc>) complexes with anti-proliferative activity bearing 4-methylene-7-methoxycoumarin (MMC) 2,6-diispropylphenyl (Dipp) substituted NHC/1,2,3-triazole hybrid ligands are described.
Identifying the covalent targets of a natural product-derivative in cancer cells.
Abstract Aqueous solutions of the achiral, monomeric, nucleobase mimics (2,4,6‐triaminopyrimidine, TAP, and a cyanuric acid derivative, CyCo6) spontaneously assemble into macroscopic homochiral domains supramolecular polymers. These assemblies exhibit high degree chiral amplification. Addition small quantity one handedness derivative CyCo6 generates exclusively structures. This system exhibits highest reported amplification for dynamic helical polymers or helices. Significantly, comprised...
Catechol-containing natural products are common constituents of foods, drinks, and drugs. Natural carrying this motif often associated with beneficial biological effects such as anticancer activity neuroprotection. However, the molecular mode action behind these properties is poorly understood. Here, we apply a mass spectrometry-based competitive chemical proteomics approach to elucidate target scope catechol-containing bioactive molecules from diverse foods Inspired by protein reactivity...
Abstract Naturstoffe sind eine ergiebige Quelle für Antibiotika, die vielfältige zelluläre Zielstrukturen adressieren. Die Erforschung ihrer Zielproteine mittels chemischer Proteomik wird häufig durch das Einbringen sterisch anspruchsvoller photoreaktiver Gruppen erschwert. Hier verwenden wir Elegaphenon, ein weitestgehend uncharakterisiertes Naturstoffantibiotikum mit einem nativen Benzophenongrundgerüst affinitätsbasiertes Protein Profiling (AfBPP) in grampositiven und gramnegativen...
In addition to their well-known role as stress-associated catecholamine hormones in animals and humans, epinephrine (EPI) norepinephrine (NE) act interkingdom signals between eukaryotic hosts bacteria. However, the molecular basis of effects on bacteria is not well understood. initial phenotypic studies utilizing Vibrio campbellii a model organism, we characterized bipartite mode action catecholamines, which consists promotion growth under iron limitation, enhanced colony expansion soft...
In addition to their well-known role as stress-associated catecholamine hormones in animals and humans, epinephrine (EPI) norepinephrine (NE) act interkingdom signals between eukaryotic hosts bacteria. However, the molecular basis of effects on bacteria is not well understood. initial phenotypic studies utilizing Vibrio campbellii a model organism, we characterized bipartite mode action catecholamines, which consists promotion growth under iron limitation, enhanced colony expansion soft...