A5053812779- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Phytochemicals and Antioxidant Activities
- Pneumonia and Respiratory Infections
- Microbial Inactivation Methods
- Fungal Biology and Applications
- Environmental and sustainability education
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Fermentation and Sensory Analysis
- Asthma and respiratory diseases
- Food Chemistry and Fat Analysis
- Seed and Plant Biochemistry
- Microbial Metabolites in Food Biotechnology
- RNA and protein synthesis mechanisms
- Natural Antidiabetic Agents Studies
- Antimicrobial Peptides and Activities
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Fungal Infections and Studies
- Infectious Diseases and Mycology
- Glycosylation and Glycoproteins Research
- Antioxidant Activity and Oxidative Stress
- Pneumocystis jirovecii pneumonia detection and treatment
Universitat de València
2003-2025
Instituto de Agroquímica y Tecnología de Alimentos
2013-2020
Consejo Superior de Investigaciones Científicas
2017-2020
Innovative processing technologies have been extensively investigated over the last decade, as a sustainable way to recover high-added-value compounds from food by-products. In this sense, these alternative optimized for variety of plant-based matrices, demonstrating higher yields compared conventional methods. However, functionality extracts obtained mainly assessed based on characterization recovered or in terms their bioactivity properties, but there is lack studies focused effect green...
We have studied the role of TLR4 in murine defenses against Candida albicans two TLR4-defective mouse strains: C3H/HeJ mice which defective signaling, and TLR4-/- knockout mice. Both strains experimentally infected with virulent C. cells showed no significant difference survival as compared their respective controls. Recruitment neutrophils to peritoneal cavity i.p. was not affected animals, but significantly enhanced mice, control In vitro production TNF-α by macrophages from both types...
Pulsed electric fields (PEF) technology has been used as a sustainable method for extracting antioxidant bioactive compounds from different food matrices. In the present study, optimal conditions of PEF extraction mushrooms (2.5 kV/cm, 50 kJ/kg, 6 h) were applied to Lentinula edodes, Agaricus brunnescens, and Pleurotus ostreatus evaluate total capacity extracts, followed by Triple TOF-LC-MS-MS analysis phenolic profile compared A. bisporus high-performance liquid chromatography coupled mass...
Immunoscreening of a Candida albicans expression library with antibodies against the 58 kDa fibrinogen-binding mannoprotein (mp58) fungus resulted in isolation clones encoding protein moiety this molecule. Sequence 0.9 kb cDNA one selected for further analysis, revealed an open reading frame coding 292 amino acids, which displays sequence similarity to proteins belonging family immunodominant antigens Aspergillus spp. The gene corresponding was named FBP1 (fibrinogen-binding protein). These...
Using a polyubiquitin cDNA as probe, we have isolated clone (pPR3, pEMBLYe23 derivative plasmid) containing the Candida albicans UBI3 gene coding for fusion protein. This protein is formed by one ubiquitin subunit fused, at its C-terminus, to an unrelated peptide which similar ribosomal encoded 3′ tail of Saccharomyces cerevisiae gene. Southern blot analysis chromosomal DNA probed with non-ubiquitin indicated that only homologous present in C. genome. Heterelogous expression pPR3 S. ubi3...
The present study addressed the protective effects against oxidative stress (OS) of a cocoa powder extract (CPEX) on protein expression profile S. cerevisiae. A proteomic analysis was performed after culture preincubation with CPEX either without (−OS) or under conditions (+OS) (5 mM H2O2). LC-MS/MS identified 33 differentially expressed proteins (–OS: 14, +OS: 19) that were included By Gene Ontology in biological processes: biosynthesis amino acids, carbohydrate metabolism and reactive...
Abstract Saccharomyces cerevisiae has been used as a model organism to study the capacity of cocoa and red grape extracts trigger an antioxidant response. A methodology adapted microtiter plates developed monitor yeast growth after culture preincubation with food ingredients exposure oxidative stress by hydrogen peroxide menadione. This proved effective in measuring ability promote response yeast, also prospect conducting dose–response studies. Additionally, method proven useful perform...
We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes ubiquitin fusion protein involved in ribosome biogenesis. A one-step disruption procedure, using plasmid pCaDis, was designed to place second copy of under control tightly regulated MET3 promoter C. heterozygous (UBI3/Δubi3::hisG), previously isolated first step ura-blaster protocol. Analysis repressing and inducing conditions indicates that is an essential whose expression required growth albicans.
La investigación en Ciencias de la Alimentación tiene cada día mayor relevancia. El mundo se enfrenta a problemas globales sin precedentes que afectan sostenibilidad alimentaria y el nexo entre alimentación salud está científicamente demostrado. Los Grados Universitarios habilitan para práctica profesional diversas áreas, pero estas no son igualmente conocidas ni contempladas por los estudiantes. Acercar Sostenible las aulas es mejor manera informar motivar elección esta área profesional. Se...
Las Naciones Unidas aprobaron en 2015 la Agenda 2030 para el desarrollo sostenible que se propusieron los 17 Objetivos de Desarrollo Sostenible (ODS) como renovación y actualización del Milenio.La Universidad debe contribuir activamente al cumplimiento ODS, dotando alumnado conocimientos, herramientas motivaciones involucre.Por ello planteó un proyecto innovación docente (UV-SFPIE_PID-1641017) persigue encajar ODS marco varias asignaturas diferentes cursos grados Ciencias Alimentación...
We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes ubiquitin fusion protein involved in ribosome biogenesis. A one-step disruption procedure, using plasmid pCaDis, was designed to place second copy of under control tightly regulated MET3 promoter C. heterozygous (UBI3/Δubi3::hisG), previously isolated first step ura-blaster protocol. Analysis repressing and inducing conditions indicates that is an essential whose expression required growth albicans.