A5101416122- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- S100 Proteins and Annexins
- Plant Virus Research Studies
- Cancer, Hypoxia, and Metabolism
- Plant-Microbe Interactions and Immunity
- Protease and Inhibitor Mechanisms
- Neuroscience and Neural Engineering
- Transgenic Plants and Applications
- Alzheimer's disease research and treatments
- Magnolia and Illicium research
- Photoreceptor and optogenetics research
- Calpain Protease Function and Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Sleep and Wakefulness Research
- Legume Nitrogen Fixing Symbiosis
- Cellular transport and secretion
- Plant tissue culture and regeneration
- Dendrimers and Hyperbranched Polymers
- Mesoporous Materials and Catalysis
- Parkinson's Disease Mechanisms and Treatments
- Synthesis and properties of polymers
- Lipid Membrane Structure and Behavior
- Acute Ischemic Stroke Management
- Amino Acid Enzymes and Metabolism
Emory University
2012-2025
First Affiliated Hospital of GuangXi Medical University
2025
Guangxi Medical University
2025
Chinese Academy of Agricultural Sciences
2025
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
2025
Sir Run Run Shaw Hospital
2008-2024
Zhejiang University
2010-2024
Shenyang Agricultural University
2024
Xuzhou Medical College
2016-2023
Gansu Agricultural University
2020-2022
Axonal injury is a common cause of neurological dysfunction. Unfortunately, in contrast to axons from the peripheral nervous system, limited capacity regeneration central system (CNS) major obstacle for functional recovery patients suffering diseases that involve subcortical white matter. Urokinase-type plasminogen activator (uPA) serine proteinase upon binding urokinase-type receptor (uPAR) catalyzes conversion into plasmin on cell surface. uPAR expression increases after an injury, and...
Experimental evidence for a molecular function gene VI of the caulimoviruses is presented. Based on experiments with figwort mosaic virus (FMV), it appears that has role in posttranscriptional expression closely packed genes (VII and I-V), which appear larger, full-length RNA transcript this virus. Gene its flanking 5'/3' signals included as separate plasmid during electroporation DNA into protoplasts Nicotiana edwardsonii shows an unusual type transactivation chloramphenicol...
Spines are dendritic protrusions that receive most of the excitatory input in brain. Early after onset cerebral ischemia spines peri-infarct cortex replaced by areas focal swelling, and their re-emergence from these varicosities is associated with neurological recovery acute ischemic stroke (AIS). Urokinase-type plasminogen activator (uPA) a serine proteinase plays central role tissue remodeling via binding to urokinase receptor (uPAR). We report cortical neurons release uPA during phase...
Urokinase-type plasminogen activator (uPA) is a serine proteinase that, upon binding to its receptor (uPAR), catalyzes the conversion of into plasmin on cell surface. Our previous studies indicate that uPA and uPAR expression increase in ischemic brain during recovery phase from an acute injury promotes neurological after stroke. Here, we used male mice genetically deficient either (uPA-/-) or (uPAR-/-) with four-amino acid substitution growth factor domain abrogates (PlatGFDhu/GFDhu)...
Morphological changes are thought to contribute the expression of long-term synaptic plasticity, a cellular basis for learning and memory.The mechanisms mediating initiation maintenance morphological poorly understood.We repeatedly imaged axonal arbors mechanosensory neurons Aplysia as they formed new varicosities branches after applications serotonin that cause facilitation.New exclusively from preexisting varicosities, by splitting or branch outgrowth.These were prevented cytocha-lasin D,...
In Aplysia, long-term facilitation (LTF) of sensory neuron synapses requires activation both protein kinase A (PKA) and mitogen-activated (MAPK). We find that 5-HT through PKA regulates secretion the neuron-specific neuropeptide sensorin, which binds autoreceptors to activate MAPK. Anti-sensorin antibody blocked LTF MAPK produced by medium containing sensorin was secreted from neurons after treatment. single application followed a 2 hr incubation with synthesis-dependent LTF, growth new...
Cerebral cortical neurons have a heightened sensitivity to hypoxia and their survival depends on ability accommodate changes in the concentration of oxygen environment. Tissue-type plasminogen activator (tPA) is serine proteinase that activates zymogen into plasmin. Hypoxia induces release tPA from cerebral neurons, it has been proposed mediates hypoxic ischemic neuronal death. Here, we show devoid neurotoxic effects instead an endogenous neuroprotectant renders resistant lethal ischemia. We...
5-HT or FMRFamide evoke long-lasting changes in the efficacy of sensorimotor (SN-L7) synapses Aplysia, structural alterations presynaptic sensory cell, and cell-specific downregulation distribution adhesion molecule apCAM. We examined how apCAM might contribute to formation new varicosities by elimination existing FMRFamide. report that is directed presence preexisting zones on motor axon are enriched for Moreover, there was a further enrichment levels at contacting beginning 1 hr lasting 24...
Protein synthesis at distal synaptic sites is thought to play a critical role in long-term plasticity preexisting connections. We tested whether protein neuritic processes contributes the formation of new connections by<i>Aplysia</i> neurons regenerating cell culture after removing their bodies. Removal either sensory neuron (SN) or motor L7 body did not affect during next 48–72 hr period. Increases efficacy removal SN was accompanied by neurite growth and an increase number varicosities...
Abstract Alzheimer’s disease (AD) is characterized by significant clinical and molecular heterogeneity, influenced genetic demographic factors. Using an unbiased, network-driven approach, we analyzed the cerebrospinal fluid (CSF) proteome from 431 individuals (483 samples), including 111 African American participants, to identify core protein modules associated with AD, race, sex, age. Our analysis revealed ten co-expression linked distinct biological pathways cell types, many of which...
Glucosinolate are one of the vital secondary metabolites in alfalfa ( Medicago sativa L.), and primarily present as β-D-glucosinolate derivatives, improving resistance response to biotic abiotic stresses alfalfa. CYP79 (Cytochrome P450 monooxygenases) CYP83 gene families play an important role core structure biosynthesis glucosinolate. Nevertheless, a comprehensive exploration family members has thus far not been study. The types glucosinolate were qualitative quantitative analysis by...
Abstract Background Amyotrophic Lateral Sclerosis (ALS), a neurodegenerative disease involving loss of motor neurons, typically results in death within 3–5 years onset. Although roughly 10% cases can be linked to specific inherited mutation (e.g., C9orf72 hexanucleotide repeat expansion or SOD1 mutation), the cause(s) most are unknown. Consequently, there is critical need for biomarkers that reflect onset and progression across ALS subgroups. Methods We employed tandem mass tag spectrometry...
Long-term facilitation of sensorimotor synapses Aplysia in culture by serotonin (5-HT) is accompanied two changes: an increase the number sensory cell branches and varicosities contacting major axons target motor L7, a downregulation adhesion molecules (apCAM) from surface presynaptic cell. We tested hypothesis that changes may be linked; 5-HT-induced decrease apCAM levels neurites defasciculate each other make more attractive substrate for new growth synapses. used developing cultures to...
Gene expression of figwort mosaic virus (FMV), a caulimovirus, was investigated by electroporation Nicotiana edwardsonii cell suspension protoplasts with cloned viral constructs in which reporter gene inserted at various positions on the genome. The results showed that genome FMV contains two promoters; one is used for production full-length RNA and another initiates synthesis separate monocistronic VI. Evidence provided transcript, probable template reverse transcription, can serve as...
Activation of several signaling pathways contributes to long-term synaptic plasticity, but how brief stimuli produce coordinated activation these is not understood. In Aplysia , the facilitation (LTF) sensory neuron synapses by 5-hydroxytryptamine (serotonin; 5-HT) requires kinases, including mitogen-activated protein kinase (MAPK). The 5-HT-enhanced secretion neuron-specific neuropeptide sensorin mediates MAPK. We find that stimulus-induced two pathways, phosphoinositide 3-kinase (PI3K) and...
The active zone (AZ) is a thickening of the presynaptic membrane where exocytosis takes place. Chemical synapses contain neurotransmitter-loaded synaptic vesicles (SVs) that at rest are tethered away from release site, but after inflow Ca +2 elicited by an action potential translocate to AZ their neurotransmitter load. We report tissue-type plasminogen activator (tPA) stored outside cerebral cortical neurons, either intermixed with small clear-core or in direct contact membrane. found...
Abnormal expression of circRNAs (circular RNAs), a subclass non-coding RNAs, has been documented in numerous human diseases. Herein, we explored whether act as ceRNAs (competing endogenous RNAs) to modulate the pathological process-insulin resistance, well dyslipidemia MetS (Metabolic Syndrome). The profile serume and control samples was characterized by circRNA deep sequencing. We identified circRNF111 key downregulated involved MetS. decreased serum directly linked excessive insulin...
Melatonin (MT) is a vital hormone factor in plant growth and development, yet its potential to influence the graft union healing process has not been reported. In this study, we examined effects of MT on oriental melon scion grafted onto squash rootstock. The studies indicate that exogenous treatment promotes lignin content stems by increasing enzyme activities hydroxycinnamoyl CoA ligase (HCT), hydroxy cinnamaldehyde dehydrogenase (HCALDH), caffeic acid/5-hydroxy-conifer aldehyde...
Tissue-type plasminogen activator (tPA) is a serine proteinase released by the presynaptic terminal of cerebral cortical neurons following membrane depolarization (Echeverry et al., 2010). Recent studies indicate that release tPA triggers synaptic vesicle cycle and promotes exocytosis (Wu 2015) endocytic retrieval (Yepes 2016) glutamate-containing vesicles. Here we used electron microscopy, proteomics, quantitative phosphoproteomics, biochemical analyses with extracts postsynaptic density...