The course of transformation DHEA, androstenediol and epiandrosterone in Mortierella isabellina AM212 culture was investigated. mentioned substrates underwent effective hydroxylation; 5-ene substrates--DHEA androstenediol--were transformed into a mixture 7α- 7β- allyl alcohols, while converted (mainly), 11α- 9α- monohydroxy derivatives. Ketoconazole cycloheximide inhibition studies suggest the presence constitutive substrate-induced hydroxylases M. isabellina. On basis time analysis...

Research on steroid biooxidation is being pursued for development of processes exploitable by the pharmaceutical industry, and also purpose preparation potentially useful analogues which are otherwise inaccessible. The use enzymes provides high regio- stereoselectivity reaction to be performed. In this work a review recent important findings related microbial enzymatic biotransformations steroidal compounds including hydroxylation, Baeyer-Villiger oxidation, dehydrogenation sterol side-chain...

The biotransformation of androsterone (1), epiandrosterone (2), androstanedione (3) and DHEA (dehydroepiandrosterone) (4) by Penicillium lanosocoeruleum—a fungal species not used in biotransformations so far—were described. All the substrates were converted high yield (70%–99%) into D ring δ-lactones. oxidation 1 produced 3α-hydroxy-17a-oxa-D-homo-5α-androstan-17-one (5). 2 led to 3β-hydroxy-17a-oxa-D-homo-5α-androstan-17-one (6). 3 resulted formation (5)...

α-Tetralone and β-tetralone were subjected to biotransformation by 14 fungal strains. Enantiomeric purity of the products depended on reaction time. 3-Day transformation α-tetralone in Absidia cylindrospora culture gave S-(+)-1,2,3,4-tetrahydro-1-naftol 92 % ee, whereas longer time resulted decrease ee value. same strain predominantly S-(-)-1,2,3,4-tetrahydro-2-naftol, after 9 days reaction, R-enantiomer with 85 was isolated. Transformation Chaetomium sp. KCh 6651 pure...

The biotransformation of steroid compounds is a promising, environmentally friendly route to new pharmaceuticals and hormones. One the reaction types common in metabolic fate steroids Baeyer-Villiger oxidation, which case cyclic ketones, such as steroids, leads lactones. Fungal enzymes catalyzing this reaction, monooxygenases (BVMOs), have been shown possess broad substrate scope, selectivity, catalytic performance competitive chemical being far more green. This study covers series...

Metabolism of steroids in healthy and unhealthy human organs is the subject extensive clinical biomedical studies. For this kind investigations, it essential that reference samples new derivatives natural, physiologically active (especially those difficult to achieve chemical synthesis) become available. This study demonstrated for first time transformation 7‐oxo‐ DHEA —a natural metabolite , using Syncephalastrum racemosum cells. The single‐pulse fermentation substrate produced two hydroxy...

This research aimed at obtaining new derivatives of pregn-1,4-diene-3,20-dione (Δ

Whole cells of the fungus Fusarium culmorum were used to carry out bioreduction 1- and 2-tetralones their derivatives with a methoxy group in aromatic ring. The process led corresponding (S)-alcohols. microbial reduction system exhibited moderate activity low enantioselectivity against 1-tetralones, but high selectivity 2-tetralones. Reduction 1-tetralones was accompanied by oxidation resulting alcohols (re-oxidation), this not observed transformations effects parameters such as...

Patchoulol was subjected to transformation by Absidia coerulea AM93 and Mucor hiemalis AM450 strains. Both micro-organisms, which displayed differing vulnerability the fungistatic action of patchoulol, were capable selective hydroxylations substrate. The major constituents mixtures after (8S)-8-hydroxypatchoulol (9R)-9-hydroxypatchoulol; carried out M. resulted in additional formation (3R)-3-hydroxypatchoulol. mixture (9R)-9-hydroxypatchoulol can be used synthesis patchoulenol, a compound...

Metabolic activities of microorganisms to modify the chemical structures organic compounds became an effective tool for production high-valued steroidal drugs or their precursors. Currently research efforts in steroids pharmaceutical interest are focused on either optimization existing processes identification novel potentially useful bioconversions. Previous studies demonstrated that P. lanosocoeruleum KCH 3012 metabolizes androstanes corresponding lactones with high yield. In order explore...

Keywords The course of transformations the pharmacological steroids: testosterone propionate, 4-chlorotestosterone acetate, 17β-estradiol diacetate and their parent alcohols in Fusarium culmorum AM282 culture was compared. results show that this microorganism is capable regioselective hydrolysis ester bonds. Only 4-ene-3-oxo steroid esters were hydrolyzed at C-17. 17β-Estradiol underwent C-3 as a result, estrone - main metabolite estradiol absent reaction mixture. resulting from oxidation...

Numerous steroids are essential plant, animal, and human hormones. The medical industrial applications of these hormones require the identification new synthetic routes, including biotransformations. metabolic fate a steroid can be complicated; it may transformed into variety substituted derivatives. This because molecule adopt several possible orientations in binding pocket receptor or an enzyme. present study, based on docking molecular dynamics, shows that is indeed for to bind site two...