Renal cell carcinoma (RCC) is the most lethal type of genitourinary cancer due to its occult onset and resistance chemotherapy radiation. Recently, accumulating evidence has suggested stains, inhibitors 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase, were associated with risk reduction cancer. In present study, we aimed investigate potential effects simvastatin on RCC cells underlying mechanisms by which exerted actions. With viability, colony formation, flow cytometric apoptosis...

The aim of this study was to establish comprehensive and practical nomograms, based on significant clinicopathological parameters, for predicting the overall survival (OS) disease-specific (DSS) patients with clear cell renal carcinoma (ccRCC).The data 35,151 ccRCC patients, diagnosed between 2004 2014, were obtained from database Surveillance, Epidemiology, End Results (SEER) program. Kaplan-Meier method Cox proportional hazards regression model used evaluate prognostic effects multiple...

Long non-coding RNAs (lncRNAs) refer to longer than 200 nucleotides in length.Numerous studies have confirmed that lncRNAs are involved almost all biological processes [7].As for tumors, play an important regulatory role tumor proliferation, survival, metastasis, and so on [8][9][10][11].Recently, the Genetics Epigenetics Research Laboratory of University Texas has demonstrated closely regulating various human cancers [12].The "star molecules" such as MALAT1, HOTAIR, H19 function oncogenes...

Background The present study aims to investigate the gene expression changes in papillary renal cell carcinoma(pRCC) and screen several genes associated pathways of carcinoma progression. Methods RNA sequencing (RNA-seq) data set was downloaded from TCGA (The Cancer Genome Atlas). We identified differentially expressed mRNAs between cancer normal tissues performed annotation figure out functions they were enriched in. Then, we constructed a risk score that relied on 5-mRNA. optimal value for...

Abstract Background The ETS transcription factor GABPA has long been thought of as an oncogenic and recently suggested a target for cancer therapy due to its critical effect on telomerase activation, but the role in clear cell renal carcinoma (ccRCC) is unclear. In addition, ccRCC characterized by metabolic reprograming with aberrant accumulation L-2-hydroxyglurate (L-2HG), oncometabolite that shown promote development progression inducing DNA methylation, however, downstream effectors...

Abstract Background Cancer-associated fibroblasts (CAFs) drive cancer progression and treatment failure on one hand, while their tumor-restraining functions are also observed the other. Recent single cell RNA sequencing (scRNA-seq) analyses demonstrates heterogeneity of CAFs defines molecular subtypes CAFs, which help explain different functions. However, it remains unclear whether these CAF have same or biological/clinical implications in prostate (PCa) other malignancies. Methods PCa cells...

The androgen receptor (AR) plays a pivotal role in prostatic carcinogenesis, and it also affects the transition from hormone sensitive prostate cancer (HSPC) to castration-resistant (CRPC). Particularly, persistent activation of appearance splicing variant 7 (AR-V7), could partly explain failure deprivation therapy (ADT). In present study, we reported that huaier extract, derived officinal fungi, has potent antiproliferative effects both HSPC CRPC cells. Mechanistically, extract...

Abstract Rationale: Inflammatory myofibroblastic tumor (IMT) is a rare intermediate soft tissue tumor. Rarely occurring in the urinary bladder, MTs composed of myofibroblast differentiated spindle cells and accompanied by numerous inflammatory cells, plasma and/or Lymphocytes. Patient concerns: A 28-year-old female, with history 2 cesarean sections 8 years ago ago, was admitted to emergency department for 7-day ongoing gross hematuria clots. Diagnoses: cystoscopy showed near bladder neck,...

RNA binding protein (RBPs) dysregulation has been reported in various malignant tumors and plays a pivotal role tumor carcinogenesis progression.However, the underlying mechanisms renal cell carcinoma (RCC) are still unknown.In present study, we performed bioinformatics analysis using data from TCGA database to explore expression prognostic value of RBPs.We identified 125 differently expressed RBPs between normal tissue RCC patients, including 87 upregulated 38 downregulated RBPs.Eight...

Telomerase activation is required for malignant transformation. Recent advances in high-throughput technologies have enabled the generation of complex datasets, thus providing alternative approaches to exploring telomerase biology more comprehensively, which has proven be challenging due need laborious assays test activity. To solve these issues, several groups analyzed TCGA pan-cancer tumor datasets by investigating reverse transcriptase (TERT), catalytic subunit activity, or its...

Tumor development and progression are influenced by macrophages of the surrounding microenvironment. To investigate influences an inflammatory tumor microenvironment on growth metastasis prostate cancer, present study used a co-culture model cancer (PCa) cells with tumor-associated macrophage (TAM)-conditioned medium (MCM). MCM promoted PCa cell (LNCaP, DU145 PC-3) growth, xenograft in nude mice consistently demonstrated that could promote growth. also stimulated migration invasion vitro....

Previous studies have demonstrated that metformin (Met) may reduce the risk of cancer development. In present study, anti-cancer effects Met in A498 cells were investigated. It was found inhibited cell proliferation a time- and dose-dependent manner, as well induced activation AMP-activated protein kinase. also promoted apoptosis mechanistic suggested this mediated by downregulation B-cell lymphoma 2 concurrent upregulation Bcl-2-associated X protein. addition, it observed G1 cycle arrest...

Abstract Background Laparoscopic adrenalectomy for pheochromocytoma is associated with high risk of intraoperative hemodynamic instability. Our study aimed to identify predictive factors instability during laparoscopic resection pheochromocytoma. Methods Between January 2011 and December 2021, 136 patients underwent unilateral The were divided into 2 groups depending on the presence or absence surgery. Intraoperative parameters compared between groups. Patient demographic characteristics...

Abstract Background Clear cell renal carcinoma (ccRCC) displays sex-biased incidence, outcomes, molecular alterations and treatment efficacy; however, clinical managements are largely identical in male female patients. Moreover, many biomarkers have been identified as predictors for ccRCC outcomes response to therapeutic drugs, such multitargeted tyrosine-kinase receptor (TKR) inhibitors, but little is known about their sex-specificity. Dyskerin (DKC1), encoded by the DKC1 gene within Xq28,...

Trametes robiniophila Murr (Huaier) has been used as an adjuvant therapy of tumor in traditional Chinese medicine for many years, but the underlying mechanisms are largely unknown. In present study, we tested inhibitory effect Huaier extract on renal cancer 786-O cells and explored possible mechanisms.

Cancer cell metabolism responsive to androgen deprivation therapy (ADT) may be involved in the development and progression of prostate cancer ultimate failure androgen-deprivation therapy. To investigate regulation effects on androgen-independent growth cancer, an established LNCaP-s model that resembles clinical scenario castration-resistant (CRPC), was used this current study. This line cultured from androgen-sensitive LNCaP parental cells, androgen-reduced condition, resembling assess...

Abstract Background On the basis of some significant clinical parameters, we had an intent to establish nomograms for estimating prognosis patients with squamous cell carcinoma urinary bladder (SCCB), including overall survival (OS) and cancer-specific (CSS). Methods The data 1210 diagnosed SCCB between 2004 2014,were obtained from Surveillance, Epidemiology, End Results (SEER) database. Cox proportional hazards regression model was applied evaluate association variables survival. Nomograms...

l-2-Hydroxyglutarate (l-2-HG) is a functionally compartmentalized metabolite involved in various physiological processes. However, its subcellular distribution and mitochondrial transport remain unclear owing to technical limitations. In the present study, an ultrasensitive l-2-HG biosensor, sfLHGFR

Studies investigating the contribution of Cytochrome P4502E1 (CYP2E1) polymorphisms to etiology urinary cancer draw inconsistent conclusions. Thus, we performed a meta-analysis evaluate association between CYP2E1 Rsa I/Pst I and Dra susceptibility.Meta-analysis based on eligible case-control studies that assess with was conducted. Subgroup analyses ethnicity type were also carried out. Odds ratios (OR) 95% confidence intervals (95% CI) calculated strength associations two polymorphisms....