A5071974166- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- Advanced Fluorescence Microscopy Techniques
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Phylogenetic Studies
- Mitochondrial Function and Pathology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
Baylor College of Medicine
2019-2024
Metastasis has been considered as the terminal step of tumor progression. However, recent genomic studies suggest that many metastases are initiated by further spread other metastases. Nevertheless, corresponding pre-clinical models lacking, and underlying mechanisms elusive. Using several approaches, including parabiosis an evolving barcode system, we demonstrated bone microenvironment facilitates breast prostate cancer cells to metastasize establish multi-organ secondary We uncovered this...
SUMMARY Metastasis has been considered as the terminal step of tumor progression. However, recent clinical studies suggest that many metastases are seeded from other metastases, rather than primary tumors. Thus, some can further spread, but corresponding pre-clinical models lacking. By using several approaches including parabiosis and an evolving barcode system, we demonstrated bone microenvironment facilitates breast prostate cancer cells to metastasize establish multi-organ secondary...
Abstract Accurate detection of somatic mutations in single tumor cells is greatly desired as it allows us to quantify the single-cell mutation burden and construct mutation-based phylogenetic tree. Here we developed scNanoSeq chemistry profiled 842 from 21 human breast cancer samples. The majority trees comprise a characteristic stem evolution followed by clonal sweep. We observed subtype-dependent lengths evolution. To explain this phenomenon, propose that differences are related different...
Somatic mutations continuously accumulate in the human genome, posing vulnerabilities towards aging and increased risk of various diseases. However, accurate detection somatic at whole genome scale is still challenging. By tagging independently sequencing two complementary strands DNA, recent development duplex-sequencing methods has greatly improved accuracy, however, limited coverage compromised compatibility with existing platforms have constrained broad applications these methods. To...
Abstract Accurate detection of somatic mutations in single tumor cells is greatly desired as it allows us to quantify the single-cell mutation burden and construct mutation-based phylogenetic tree. Here we developed scNanoSeq chemistry profiled 842 from 21 human breast cancer samples. The majority trees comprise a characteristic stem evolution followed by clonal sweep. We observed subtype-dependent lengths evolution. To explain this phenomenon, propose that differences are related different...
Abstract In the studies of single-cell genomics, large endeavor has been focused on detection permanent changes in genome. On other hand, spontaneous DNA damage frequently occurs and results transient single-stranded to genome until they are repaired. So far, successful profiling these dynamic not demonstrated by whole-genome amplification methods. Here we reported a novel WGA method: Linearly Produced Semiamplicon based Split Amplification Reaction (LPSSAR), which allows, for first time,...