A5011976234- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Adaptive optics and wavefront sensing
- Japanese History and Culture
- Ion channel regulation and function
- Astronomy and Astrophysical Research
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Connexins and lens biology
- Calibration and Measurement Techniques
- Superconducting Materials and Applications
- Electric Motor Design and Analysis
- Astronomical Observations and Instrumentation
- Stellar, planetary, and galactic studies
- Neural dynamics and brain function
- Rangeland Management and Livestock Ecology
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced MEMS and NEMS Technologies
- Genetic diversity and population structure
- Growth Hormone and Insulin-like Growth Factors
- Vestibular and auditory disorders
- Action Observation and Synchronization
- Tourism, Volunteerism, and Development
Juntendo University
2012-2025
Toyo University
2023
Kyushu University
1994-2022
Tokyo University of Foreign Studies
2019-2021
Niigata University
1986-2021
Waseda University
2021
Musashino Art University
2021
National Astronomical Observatory of Japan
1994-2020
National Clonal Germplasm Repository
2011-2019
Kyoto University
2006-2017
Type 2 and type 3 inositol 1,4,5-trisphosphate receptors (IP3R2 IP3R3) are intracellular calcium-release channels whose physiological roles unknown. We show exocrine dysfunction in IP3R2 IP3R3 double knock-out mice, which caused difficulties nutrient digestion. Severely impaired calcium signaling acinar cells of the salivary glands pancreas mutants ascribed secretion deficits to a lack release. Despite normal caloric intake, were hypoglycemic lean. These results reveal as key molecules...
Attachment between mothers and infants is the most primitive primary form of human social relationship. Many reports have suggested that orbitofrontal cortex (OFC) plays a significant role in this attachment; however, only select few provide experimental neurophysiological evidence. In present study, to determine neural substrates underlying emotional attachment infants, we measured their prefrontal activation by using near-infrared spectroscopy. We used movie stimuli could robustly induce...
Topoisomerase inhibitors such as camptothecin and etoposide are used anti-cancer drugs induce double-strand breaks (DSBs) in genomic DNA cycling cells. These DSBs often covalently bound with polypeptides at the 3' 5' ends. Such modifications must be eliminated before DSB repair can take place, but it remains elusive which nucleases involved this process. Previous studies show that CtIP plays a critical role generation of single-strand overhang "clean" DSBs, thus initiating homologous...
We examined the properties of [Ca 2+ ] i changes that were evoked by backpropagating action potentials in pyramidal neurons hippocampal slices from rat. In presence metabotropic glutamate receptor (mGluR) agonists t- ACPD, DHPG, or CHPG, spikes caused Ca waves initiated proximal apical dendrites and spread over this region soma. Consistent with previously described synaptic responses (Nakamura et al., 1999a), pharmacological experiments established attributable to release internal stores...
An overview of the current status 8.2 m Subaru Telescope constructed and operated at Mauna Kea, Hawaii, by National Astronomical Observatory Japan is presented. The basic design concept verified performance telescope system are described. Also given instrument package offered to astronomical community, operation, some future plans. reported in a number SPIE papers as summer 2002 incorporated with updates included 2004 February. However, readers encouraged check most updated through home...
The expression of calreticulin, a Ca(2+)-binding chaperone the endoplasmic reticulum, is elevated in embryonic heart, and because impaired cardiac development, knockout Calreticulin gene lethal during embryogenesis. downregulated after birth. Here we have investigated physiological consequences continued high calreticulin postnatal by producing transgenic mice that overexpress protein heart. These animals exhibit decreased systolic function inward I(Ca,L), low levels connexin43 connexin40,...
In this study, we investigated the effects of a short-term and long-term high-fat diet (HFD) on morphological functional features fast-twitch skeletal muscle. Male C57BL/6J mice were fed HFD (60% fat) for 4 weeks (4-week HFD) or 12 (12-week HFD). Subsequently, extensor digitorum longus muscle was isolated, composition fiber type, expression levels proteins involved in contraction, force production electrical stimulation analyzed. The 12-week HFD, but not 4-week resulted decreased tetanic 100...
Postsynaptic [Ca2+]i increases result from Ca2+ entry through ligand-gated channels, voltage-gated or release intracellular stores. We found that these sources have distinct spatial distributions in hippocampal CA1 pyramidal neurons. Large amplitude regenerative of IP3-sensitive stores the form waves were almost exclusively on thick apical shaft. Smaller events did not extend more than 15 microm into oblique dendrites. These synaptically activated initiated at points where stimulated...
beta-Amylase of sweet potato (Ipomoea batatas L.), which constitutes about 5% the total soluble protein tuberous root, is absent or present in only small amounts organs other than roots normal, field-grown plants. However, when leaf-petiole cuttings from such plants were supplied with a solution that contained sucrose, accumulation beta-amylase was induced both leaf and petiole portions explants. The sucrose-induced occurred concomitant starch sporamin, most abundant storage root....
An mGluR5-selective diffusion barrier enriches mGluR5 in astrocytic processes, enabling compartmentalized calcium signaling.
Although it has long been believed that glial cells play a major role in transmitter uptake at synapses the CNS, relative contribution of and neuronal to reuptake synaptically released glutamate unclear. Recent identification diverse transporter subtypes provides an opportunity examine this issue. To monitor activity, we optically detected induced changes membrane potential from hippocampal CA1 field slice preparations using voltage-sensitive dye, RH155. In presence ionotropic...
In this paper, we present the design and test performance of COMICS, mid-IR instrument for 8.2 m Subaru Telescope at Mauna Kea. The has both imaging long slit grating spectroscopy capabilities in 8-26 micrometers wavelength range. camera section, there are selectable three sets lens assembly, one 10 imaging, another 20 other pupil imaging. This section an SBRC 320 X 240 Si:As IBC array serves as a viewer pixel scale 0.130 arcsec. spectrograph is designed to have fiber arrays. Five arrays...
This paper describes the first light and subsequent test observations with 8.2 m aperture Subaru Telescope constructed at summit of Mauna Kea. Following engineering light, which started 1998 December, astronomical were carried out in 1999 January 4 testing instruments under seeing conditions 0″.2*#x2013;0″.5 for near-infrared 0″.3–0″.6 optical wavelengths. The actively supported primary mirror was shown to achieve an overall imaging performance 0″.1 (FWHM) or better absence any atmospheric...
Rapid and convenient structural analysis of neutral glycosphingolipids (GSLs) was achieved by direct coupling thin-layer chromatography (TLC) to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-TOF) MS/MS. Positions unstained GSL spots on developed TLC plates were determined comparison orcinol-stained references. A matrix solution 2,5-dihydroxybenzoic acid (DHB) in acetonitrile/water (1:1 v/v) then added directly the spots, GSLs analyzed MALDI-QIT-TOF...
The sugar-inducible expression of genes for sporamin and [beta]-amylase in leaf explants sweet potato (Ipomoea batatas) that a [beta]-glucuronidase-fusion gene, with the promoter gene leaves tobacco (Nicotiana tabacum), requires Ca2+ signaling (M. Ohto, K. Hayashi, M. Isobe, Nakamura [1995] Plant J 7: 297-307), it was inhibited by staurosporin K252a, inhibitors protein kinases. Autophosphorylation activities several potential kinases were significantly higher younger than mature leaves....
A new ganglioside showing choleragenoid-binding activity was purified from mouse spleen and characterized. From the results of sugar-composition analysis, enzymatic hydrolysis, a permethylation study, 1 H-NMR spectroscopy, negative-ion fast atom bombardment mass spectrometry, structure determined to be as follows: This contains terminal tetrasaccharide identical with that II 3 NeuGcα-Gg 4 Cer (GM1(NeuGc)). By means TLC-immunobinding assay an enzyme-linked immunosorbent assay, demonstrated...
In previous studies it was demonstrated that there are three variations as to the expression of liver gangliosides in inbred strains mice; first group expresses GM3(NeuGc) a major component, second group, GM2(NeuGc), and third GM1 (NeuGc), GD1a(NeuGc). present study, we attempted determine which organs, if any, exhibit same polymorphic those observed liver. Thus, spleen, thymus, heart, lung, kidney, testis, erythrocytes, well liver, were examined using TLC-mapping technique or by...