A5079102502- Enzyme Structure and Function
- Hydrogels: synthesis, properties, applications
- Protein Structure and Dynamics
- Protein Kinase Regulation and GTPase Signaling
- Nuclear Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- PI3K/AKT/mTOR signaling in cancer
- Glycosylation and Glycoproteins Research
- Heme Oxygenase-1 and Carbon Monoxide
- Hemoglobin structure and function
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Protein purification and stability
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Neonatal Health and Biochemistry
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
RIKEN Advanced Science Institute
2009-2025
RIKEN Center for Emergent Matter Science
2017-2025
Pioneer (Japan)
2021-2025
RIKEN
2013-2024
Saitama University
2021-2022
Pioneer (United States)
2019-2020
Nippon Soken (Japan)
2020
Chinese Academy of Sciences
2017
Changchun Institute of Applied Chemistry
2017
Jilin University
2017
Significance Cells use chemical signals for intracellular communication in our bodies. Inositol 1,4,5-trisphosphate (IP 3 ) is a signal that binds to the IP receptor R) release calcium ions from endoplasmic reticulum. The distance ligand-binding sites channel within R longest among known ligand-gated ion channels, and fundamental question of how -binding physically opens remains unanswered. Here, we solved -bound unbound structures large cytosolic domains by X-ray crystallography clarified...
Pirin is a newly identified nuclear protein that interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and factor I (NFI). The crystal structure of human at 2.1-Å resolution shows it to be member functionally diverse cupin superfamily. comprises two β-barrel domains, an Fe(II) cofactor bound within cavity N-terminal domain. These findings suggest enzymatic role for Pirin, most likely in biological redox reactions involving oxygen, provide compelling evidence requires...
We identified a novel, nontoxic mushroom protein that specifically binds to complex of sphingomyelin (SM), major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains an SM- Chol-dependent manner decorated specific lipid colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. use nakanori as lipid-domain–specific probe revealed altered distribution dynamics SM/Chol on the Niemann-Pick type C fibro-blasts,...
In this study, we determined the crystal structure of N-terminal importin-β-binding domain (IBB)-truncated human importin-α1 (ΔIBB-h-importin-α1) at 2.63 Å resolution. The ΔIBB-h-importin-α1 reveals a novel closed homodimer. homodimer exists in an autoinhibited state which both major and minor nuclear localization signal (NLS) binding sites are completely buried homodimerization interface, arrangement that restricts NLS binding. Analytical ultracentrifugation studies revealed is equilibrium...
Abstract The generation of metal surfaces with biological properties, such as cell‐growth‐enhancing and differentiation‐inducing abilities, could be potentially exciting for the development functional materials use in humans, including artificial dental implants joint replacements. However, currently immobilization proteins on metals are limited. In this study, we have used a mussel‐inspired bioorthogonal approach to design 3,4‐hydroxyphenalyalanine‐containing recombinant insulin‐like...
The CO-sensing transcriptional activator CooA contains a six-coordinate protoheme as CO sensor. Cys75 and His77 are assigned to the fifth ligand of ferric ferrous hemes, respectively. In this study, we carried out alanine-scanning mutagenesis EXAFS analyses determine coordination structure heme in CooA. Pro2 is thought be sixth hemes CooA, which consistent with crystal (Lanzilotta, W. N., Schuller, D. J., Thorsteinsson, M. V., Kerby, R. L., Roberts, G. P., Poulos, T. L. (2000) Nat. Struct....
Abstract Initiated by the specific binding between D‐glucose and biological receptors, human body has a delicate metabolic system to regulate blood levels, but failing release insulin would induce hyperglycemia or type I diabetes. While delivery is an effective form of therapy, self‐regulated triggering for on‐demand supplementation remains inadequate. Here, biomimetic glucose trigger‐insulin system, that is, bidentate β‐cyclodextrin‐based hydrogel with preloaded presented; dual shows...
FixL is a heme-based O2 sensor protein involved in two-component system of symbiotic bacterium. In the present study, iron coordination structure heme domain Rhizobium meliloti FixLT (RmFixLT, soluble truncated FixL) was examined using Fe K-edge extended x-ray absorption fine (EXAFS) and resonance Raman spectroscopic techniques. EXAFS analyses, interatomic distances angles Fe-ligand bond displacement from plane were obtained for RmFixLT Fe2+, Fe2+O2, Fe2+CO, Fe3+, Fe3+F−, Fe3+CN− states. An...
The crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IF03080 has been determined at 2.0 A resolution by X-ray method. enzyme consists N-terminal catalytic and C-terminal β-helix domains. On structural comparison between present structurally-known liganded enzyme, some changes were observed around active site. In Y216 serves as fifth ligand for site zinc ion. binding, may move to form a hydrogen-bond with carbonyl oxygen P1 residue peptide. D191 in flexible...
VEGF isoforms immobilised by photo-reactive gelatin (AzPhe-gelatin) enhance cell migration and proliferation.