A5100445130- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Spectroscopy and Chemometric Analyses
- DNA and Nucleic Acid Chemistry
- Optical Coherence Tomography Applications
- Nanoplatforms for cancer theranostics
- Molecular Biology Techniques and Applications
- Sarcoma Diagnosis and Treatment
- Click Chemistry and Applications
- Medical Imaging Techniques and Applications
- Respiratory viral infections research
- Advanced Electron Microscopy Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Epigenetics and DNA Methylation
- Gold and Silver Nanoparticles Synthesis and Applications
- Autophagy in Disease and Therapy
- Reproductive Biology and Fertility
- Virus-based gene therapy research
Center for Systems Biology
2017-2024
Harvard University
2017-2024
Nanjing University
2020-2024
The University of Texas MD Anderson Cancer Center
2009-2024
Chinese Academy of Sciences
2016-2024
Shenzhen University
2017-2024
Shenzhen Institutes of Advanced Technology
2023-2024
Collaborative Innovation Center of Advanced Microstructures
2024
Guangzhou Quality Supervision, Inspection and Research Institute
2021-2024
Zhujiang Hospital
2023
Transformative technologies are enabling the construction of three dimensional (3D) maps tissues with unprecedented spatial and molecular resolution. Over next seven years, NIH Common Fund Human Biomolecular Atlas Program (HuBMAP) intends to develop a widely accessible framework for comprehensively mapping human body at single-cell resolution by supporting technology development, data acquisition, detailed mapping. HuBMAP will integrate its efforts other funding agencies, programs,...
We report the development of multiplexed cellular super-resolution imaging using DNA-barcoded binders.
To decipher the molecular mechanisms of biological function, it is critical to map composition individual cells or even more importantly tissue samples in context their environment situ. Immunofluorescence (IF) provides specific labeling for profiling. However, conventional IF methods have finite multiplexing capabilities due spectral overlap fluorophores. Various sequential imaging been developed circumvent this limit but are not widely adopted common limitation requiring multirounds slow...
The relentless evolution of SARS-CoV-2 poses a significant threat to public health, as it adapts immune pressure from vaccines and natural infections. Gaining insights into potential antigenic changes is critical but challenging due the vast sequence space. Here, we introduce Machine Learning-guided Antigenic Evolution Prediction (MLAEP), which combines structure modeling, multi-task learning, genetic algorithms predict viral fitness landscape explore via in silico directed evolution. By...
In this work, a novel MXene-Au nanoparticle (Ti3C2@Au) was synthesized with high molar extinction coefficient, strong fluorescence quenching ability, ultrahigh antibody affinity, stability, and good dispersibility, it used to develop colorimetric-fluorescence dual-mode lateral flow immunoassay (LFIA). The detection limits of method for the dexamethasone in milk, beef, pork were 0.0018, 0.12, 0.084 μg/kg "turn-off" mode (colorimetric signal), 0.0013, 0.080, 0.070 "turn-on" (fluorescent...
There is currently a lack of efficacious treatments for patients with chemo-resistant small-cell lung cancer (SCLC), leading to poor prognoses. We examined SCLC cell line using genome-wide CRISPR/Cas9 screening and identified serine/threonine kinase division cycle 7 (CDC7) as potential synergistic target. Silencing CDC7 in cells decreased the IC50 improved efficacy chemotherapy. Based on highest single agent model, inhibitor XL413 had effect both cisplatin etoposide cells, but no such...
Antibodies have long served as vital tools in biological and clinical laboratories for the specific detection of proteins. Conventional methods employ fluorophore or horseradish peroxidase-conjugated antibodies to detect signals. More recently, DNA-conjugated emerged a promising technology, capitalizing on programmability amplification capabilities DNA enable highly multiplexed ultrasensitive protein detection. However, nonspecific binding has impeded widespread adoption this approach. Here,...
Abstract Autophagy can sustain or kill tumor cells depending upon the context. The mechanism of autophagy-associated cell death has not been well elucidated and autophagy enhanced inhibited sensitivity cancer to cytotoxic chemotherapy in different models. ARHI ( DIRAS3 ), an imprinted suppressor gene, is downregulated 60% ovarian cancers. In culture, re-expression induces within 72 h. xenografts, arrests growth autophagy, but does engrafted cells. When levels are reduced after 6 weeks,...
The development of super-resolution fluorescence microscopy has overcome the resolution limit conventional confocal (∼250 nm), enabling more comprehensive and accurate studies behavior nanomaterials in intracellular microenvironment.
Abstract With exceptional photochromic and photoluminescent properties, spiropyrans have demonstrated significant potential for advanced information encryption anti‐counterfeiting applications. However, its inherent water insolubility leads to incompatibility with aqueous polymers, even more, ease of leaching from the matrix hinders formation stable stimuli‐responsive platforms through direct blending all polymers. Here, fabrication amphiphilic spiropyran‐octapeptide molecules is reported...
Abstract The generation of metal surfaces with biological properties, such as cell‐growth‐enhancing and differentiation‐inducing abilities, could be potentially exciting for the development functional materials use in humans, including artificial dental implants joint replacements. However, currently immobilization proteins on metals are limited. In this study, we have used a mussel‐inspired bioorthogonal approach to design 3,4‐hydroxyphenalyalanine‐containing recombinant insulin‐like...
Mitochondrial respiratory dysfunction has intimate relationship with redox regulation. The key mechanism about how the mitochondrial respiration-defective cells survive oxidative stress is still elusive. Here, we report that transcription factor zinc-finger protein 143 (ZNF143) expression and glutathione peroxidase (GPX) activity are markedly increased in respiratory-defective induced by dominant-negative DNA polymerase γ (POLGdn). In this work, investigation of cellular antioxidant (GSH)...
Abstract Background Tissue-clearing techniques have recently been developed to make tissues transparent for three-dimensional (3D) imaging at different scales, including single-cell resolution. However, current tissue-clearing workflows several disadvantages, complex protocols, time-consuming application, and fluorescence quenching. Additionally, they can be used mainly clearing larger-volume samples, preventing wide easy applicability in conventional experimental approaches. In this study,...
Abstract Gene heterogeneity leads to the differences in cellular behaviors a wide range, such as tumor drug‐resistant mutation, epithelial‐mesenchymal transition, and migration, posing significant challenges development of biomedicine. Traditional gene analysis methods, polymerase chain reaction, employ mass cells source, resulting that properties from specific single cell are hidden massive information. Recent decades have seen emerging single‐cell techniques with their unprecedented...
Cell misuse and cross-contamination can affect the accuracy of cell research results result in wasted time, manpower material resources. Thus, line identification is important necessary. At present, commonly used methods need staining culturing. There therefore a to develop new method for rapid automated lines. Raman spectroscopy has become one emerging techniques field microbial identification, with advantages being noninvasive providing molecular information biological samples, which...
An improved sensitivity of an ATP detection assay was achieved by using allosteric probe and exonuclease III digestion product-triggered signal amplification.
We reduce the intensity noise of laser light by using an electro-optic modulator and acousto-optic in series. The reduces at high frequency (10 kHz to 1 MHz), while sets average power low (up 10 kHz). is then used trap single sodium atoms optical tweezer, where lifetime limited parametric heating due twice trapping frequency. With our eater, reduced up 15 dB these frequencies atom tweezer increased order magnitude around 6 seconds. Our technique general acts directly on beam, expanding...