A5088086867- Ovarian cancer diagnosis and treatment
- Autophagy in Disease and Therapy
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- PARP inhibition in cancer therapy
- Genetic Syndromes and Imprinting
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Cancer Mechanisms and Therapy
- Cancer, Lipids, and Metabolism
- Cancer Research and Treatment
- Cancer Treatment and Pharmacology
- Renal and related cancers
- Renal cell carcinoma treatment
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Colorectal Cancer Treatments and Studies
- Prenatal Screening and Diagnostics
- Cancer Research and Treatments
- Advanced Biosensing Techniques and Applications
- Colorectal Cancer Surgical Treatments
- Cancer-related gene regulation
The University of Texas MD Anderson Cancer Center
2016-2025
Shenzhen University
2025
Fuzhou University
2023-2025
Sun Yat-sen University
2008-2025
China Pharmaceutical University
2025
China Medical University
2009-2025
University of Science and Technology of China
2022-2024
First Affiliated Hospital of Xiamen University
2020-2024
Jiangxi University of Traditional Chinese Medicine
2024
Donghua University
2023-2024
The role of autophagy in oncogenesis remains ambiguous, and mechanisms that induce regulate its outcome human cancers are poorly understood. maternally imprinted Ras-related tumor suppressor gene aplasia Ras homolog member I (ARHI; also known as DIRAS3) is downregulated more than 60% ovarian cancers, here we show re-expression ARHI multiple cancer cell lines induces by blocking PI3K signaling inhibiting mammalian target rapamycin (mTOR), upregulating ATG4, colocalizing with cleaved...
Specification of Hsp70 action in cellular protein metabolism may occur through the formation specialized Hsp70:Hsp40 pairs. To test this model, we compared ability purified Sis1 and Ydj1 to regulate ATPase protein-folding activity Ssa1 Ssb1/2 proteins. could both functionally interact with Ssa1, but not proteins, refold luciferase. Interestingly, Ydj1:Ssa1 promote up four times more luciferase folding than Sis1:Ssa1. This functional difference was explored be accounted for by differences...
Imprinted tumor suppressor genes may be particularly important in the pathogenesis of ovarian cancer. Two imprinted genes, paternally expressed 3 (PEG3) and aplasia Ras homologue member I (ARHI), are most frequently down-regulated cancers on gene expression arrays.PEG3 ARHI levels were evaluated with real-time reverse-transcriptase polymerase chain reaction (PCR) analysis. Promoter methylation was measured by pyrosequencing, loss heterozygosity (LOH) detected PCR-LOH assays.PEG3 75% 88% 40...
Epigenetic therapy has had a significant impact on the management of hematologic malignancies, but its role in treatment ovarian cancer remains to be defined. The authors previously demonstrated that and breast cells with DNA methyltransferase histone deacetylase (HDAC) inhibitors can up-regulate expression imprinted tumor suppressors. In this study, demethylating agents HDAC were tested for their ability induce re-expression suppressor genes, inhibiting growth culture xenografts.Ovarian...
Signal transducer and activator of transcription 3 (Stat3), a target for anticancer drug design, is activated by recruitment to phosphotyrosine residues on growth factor cytokine receptors via its SH2 domain. We report here structure-activity relationship studies phosphopeptide mimics targeted the domain Stat3. Inclusion methyl group β-position pTyr mimic 4-phosphocinnamide enhanced affinity 2- 3-fold. Bis-pivaloyloxymethyl prodrugs containing β-methylcinnamide, dipeptide scaffolds Haic...
Abstract Early detection of ovarian cancer remains an important unmet medical need. Effective screening could reduce mortality by 10%–30%. Used individually, neither serum CA125 nor transvaginal sonography (TVS) is sufficiently sensitive or specific. Two-stage strategies have proven more effective, where a significant rise above woman's baseline prompts TVS and abnormal sonogram surgery. Two major trials documented that this strategy has adequate specificity, but sensitivity for early-stage...
The Normal Risk Ovarian Screening Study (NROSS) tested a two-stage screening strategy in postmenopausal women at conventional hereditary risk where significantly rising cancer antigen (CA)-125 prompted transvaginal sonography (TVS) and abnormal TVS surgery to detect ovarian cancer.
Ydj1 is a member of the Hsp40 (DnaJ-related) chaperone family that facilitates cellular protein folding by regulating Hsp70 ATPase activity and binding unfolded polypeptides. contains four conserved subdomains appear to represent functional units. To define action these regions, protease-resistant fragments mutants were analyzed for activities exhibited unmodified protein. The mutant proteins unable support growth yeast at elevated temperatures found have alterations in J-domain (Ydj1 H34Q),...
Purpose: Salt-inducible kinase 2 (SIK2) is a centrosome required for mitotic spindle formation and potential target ovarian cancer therapy. Here, we examine the effects of novel small-molecule SIK2 inhibitor, ARN-3236, on sensitivity to paclitaxel in cancer.Experimental Design: expression was determined tissue samples cell lines. ARN-3236 tested its efficiency inhibit growth enhance cultures xenografts siRNA were compared their ability produce nuclear-centrosome dissociation, splitting,...
Poly(adenosine diphosphate ribose) polymerase (PARP) inhibitors exhibit promising activity against ovarian cancers, but their efficacy can be limited by acquired drug resistance. This study explores the role of autophagy in regulating sensitivity cancer cells to PARP inhibitors.Induction was detected punctate LC3 fluorescence staining, LC3I LC3II conversion on Western blot analysis, and electron microscopy. Enhanced growth inhibition apoptosis were observed when used with hydroxychloroquine,...
Oncogenic RAS mutations drive cancers at many sites. Recent reports suggest that dimerization, multimerization, and clustering correlate strongly with activation of signaling. We have found re-expression DIRAS3, a RAS-related small GTPase tumor suppressor is downregulated in multiple cancers, inhibits RAS/mitogen-activated protein kinase (MAPK) signaling by interacting directly RAS-forming heteromers, disrupting clustering, inhibiting Raf activation, transformation growth cancer cells...
The LC3/GABARAP family of proteins is involved in nearly every stage autophagy. Inhibition a promising approach to blocking autophagy, which sensitizes advanced cancers DNA-damaging chemotherapy. Here, we report the structure-based design stapled peptides that inhibit GABARAP with nanomolar affinities. Small changes staple structure produced very different binding modes and functional differences paralog selectivity, ranging from highly GABARAP-specific broad inhibition both subfamilies....