A5079705598- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- HIV/AIDS Research and Interventions
- Bacteriophages and microbial interactions
- Viral Infections and Outbreaks Research
- Pancreatic function and diabetes
- Blood groups and transfusion
- Vibrio bacteria research studies
- HIV, Drug Use, Sexual Risk
- Adolescent Sexual and Reproductive Health
- Alcohol Consumption and Health Effects
- Vaccine Coverage and Hesitancy
- HIV/AIDS Impact and Responses
- Molecular Biology Techniques and Applications
- Cytomegalovirus and herpesvirus research
The Francis Crick Institute
2023-2025
Abstract Hepatitis B virus (HBV) whole genome sequencing (WGS) is currently limited as the DNA viral loads (VL) of many clinical samples are below threshold required to generate full genomes using current methods. We developed two pan-genotypic enrichment methods, probe-based capture and tiled amplicon PCR (HEP-TILE) for HBV WGS. demonstrate mock that both methods (genotypes A-J). Using samples, we HEP-TILE amplification successfully amplifies at lowest VL tested (30 IU/ml), products can be...
Chronic hepatitis B virus (HBV) infection is a major risk factor for hepatocellular carcinoma (HCC). In African populations, HCC frequently presents at an advanced stage with poor outcomes. We applied whole genome sequencing (WGS) to compare HBV genomes in individuals and without HCC. identified adults infection, complicating HCC, Cape Town, South Africa. generated WGS using pan-genotypic probe-based enrichment followed by Illumina sequencing. Compared the non-HCC group, patients were more...
Background: The World Health Organization (WHO) 2024 Hepatitis B virus (HBV) treatment guidelines expand eligibility for nucleos(t)ide analogue in individuals with chronic HBV infection. For countries to implement these guidelines, there is a critical need understand the population who are eligible. While drug resistance (HBVDR) uncommon, monitoring any potential relevant public health consideration. Methods: We studied rural Uganda describe proportion of eligible based on WHO guidelines....
Abstract Markers of liver and metabolic disease have not been well described for African populations but could be crucial to inform population health interventions that reduce morbidity mortality. We studied a in rural Uganda describe the baseline longitudinal distributions function tests, investigate how liver, cardiovascular parameters are associated with all-cause Demographic laboratory data were collected through General Population Cohort (GPC) South-Western Uganda. summarised cohort...
Diagnosing and treating chronic hepatitis B virus (HBV) infection are key interventions to support progress towards elimination of viral by 2030. Although nucleos/tide analogue (NA) therapy is typically highly effective, challenges remain for load (VL) suppression, including medication access, incomplete adherence drug resistance. We present a case long-term HBV HIV coinfected adult prescribed with sequential NA regimens, episodes breakthrough viraemia. Multiple factors contribute...
ABSTRACT Hepatitis B Virus (HBV) genome sequencing can be used to provide more complete genetic information at the population and individual level shed light on limitations of current interventions, inform new strategies for elimination. HBV is challenging due partially dsDNA genome, high diversity, low viral loads presence large amounts host material in clinical samples. Here we describe design use a pan-genotypic panel 74 specific capture-probes nuclease treatment improving efficiency. We...
Hepatitis B virus (HBV) whole genome sequencing (WGS) is currently limited as the DNA viral loads (VL) of many clinical samples are below threshold required to generate full genomes using current methods. We developed two pan-genotypic enrichment methods, probe-based capture and tiled amplicon PCR (HEP-TILE) for HBV WGS. demonstrate mock that both methods (genotypes A-J). Using samples, we HEP-TILE amplification successfully amplifies at lowest VL tested (30 IU/ml), products can be sequenced...
Background: Hepatitis B virus (HBV) is a neglected public health threat with poor community awareness and access to prevention, despite having safe effective vaccine. There are still gaps in diagnosis treatment, particularly the World Health Organization (WHO) African region. New WHO HBV guidelines, for first time, include use of dual therapy treatment (Tenofovir (TDF) Emtricitabine or Lamivudine (XTC) due challenges accessing TDF monotherapy. TDF/XTC also recommended as Pre-Exposure...
ABSTRACT Aim Chronic hepatitis B virus (HBV) infection is a major risk factor for hepatocellular carcinoma (HCC) particularly in African populations, whom malignancy frequently presents at an advanced stage with poor outcomes. We derived HBV whole genome sequences (WGS) from individuals HCC and compared them to without HCC. Methods: identified adults infection, complicating HCC, Cape Town, South Africa utilized pan-genotypic probe-based enrichment followed by Illumina sequencing derive WGS....