A5053887032- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Galectins and Cancer Biology
- PARP inhibition in cancer therapy
- Electrostatic Discharge in Electronics
- Oral microbiology and periodontitis research
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- Integrated Circuits and Semiconductor Failure Analysis
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- Cancer Mechanisms and Therapy
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- PI3K/AKT/mTOR signaling in cancer
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Fungal and yeast genetics research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genetic and phenotypic traits in livestock
- Electron Spin Resonance Studies
AstraZeneca (United Kingdom)
2018-2024
European Molecular Biology Laboratory
2015-2017
UiT The Arctic University of Norway
2010-2012
Universidade Federal do Ceará
2005-2011
Abstract Extracellular vesicles (EVs) have important roles in physiology, pathology, and more recently been identified as efficient carriers of therapeutic cargoes. For study EVs, a single-step, rapid scalable isolation strategy is necessary. Chromatography techniques are widely used for biological material clinical applications EVs net negative charge, anion exchange chromatography (AIEX) strong candidate column based EV isolation. We isolated by AIEX compared them to ultracentrifugation...
Non-homologous end joining (NHEJ) is one of two critical mechanisms utilized in humans to repair DNA double-strand breaks (DSBs). Unrepaired or incorrect DSBs can lead apoptosis cancer. NHEJ involves several proteins, including the Ku70/80 heterodimer, DNA-dependent protein kinase catalytic subunit (DNA-PKcs), X-ray cross-complementing 4 (XRCC4), XRCC4-like factor (XLF), and ligase IV. These core proteins bind ligate damaged ends. However, details structural assembly these remain unclear....
The target of rapamycin (TOR) kinase assembles into two distinct multiprotein complexes, conserved across eukaryote evolution. In contrast to TOR complex 1 (TORC1), TORC2 activity is not inhibited by the macrolide rapamycin. Here, we present structure Saccharomyces cerevisiae determined electron cryo-microscopy. contains six subunits assembling a 1.4 MDa rhombohedron. Tor2 and Lst8 form common core both complexes. Avo3/Rictor unique TORC2, but interacts with same HEAT repeats that are...
Lectins are mainly described as simple carbohydrate-binding proteins. Previous studies have tried to identify other binding sites, which possible recognize plant hormones, secondary metabolites, and isolated amino acid residues. We report the crystal structure of a lectin from Canavalia gladiata seeds (CGL), describing new pocket, may be related pathogen resistance activity in ConA-like lectins; site where non-protein amino-acid, α-aminobutyric (Abu), is bound. The overall native CGL...
BackgroundPLCγ enzymes are key nodes in cellular signal transduction and their mutated rare variants have been recently implicated development of a range diseases with unmet need including cancer, complex immune disorders, inflammation neurodegenerative diseases. However, molecular nature activation the impact dysregulation mechanisms by mutations, remain unclear; both critically dependent on comprehensive characterization intact PLCγ enzymes.MethodsFor structural studies we applied cryo-EM,...
Poly(ADP-ribose) polymerase 1 (PARP1) is an abundant nuclear enzyme that plays important roles in DNA repair, chromatin organization and transcription regulation. Although binding activation of PARP1 by damage sites has been extensively studied, little known about how binds to long stretches undamaged it could shape architecture. Here, using single-molecule techniques, we show condenses undamaged, kilobase-length subject sub-piconewton mechanical forces. Stepwise decondensation at high force...
Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection anti-inflammatory systemic injection. Protein sequencing was obtained Tandem Mass Spectrometry crystal structure solved X-ray crystallography using a Synchrotron radiation source. Molecular...
Atomic force microscopy (AFM) has demonstrated the ability to resolve single DNA molecules in liquid at a spatial resolution that is sufficient visualize double helix structure and variations therein. Such can be due inherent configurational flexibility may related to, e.g., sequence, ionic screening, supercoiling, or protein binding. These AFM experiments require adhered solid preferably flat support. For high-resolution, in-liquid studies so far, such adhesion commonly been achieved using...
A sala de curativos é o setor uma unidade saúde onde são dispensados os cuidados aos clientes com feridas. O enfermeiro como gestor do cuidado responsável pelo sucesso desse processo. objetivo geral desta pesquisa analisar, por meio revisão bibliográfica, as práticas e desafios enfrentados pela enfermagem na gestão da em Unidades Básicas Saúde, foco organização cuidado, uso recursos qualidade assistência. Este estudo foi conduzido integrativa, permitindo análise abrangente sobre a atuação...
Aim: Initial colonization of the tooth surface by streptococci involves attachment these bacteria to adsorbed salivary components acquired pellicle. In dental biofilm this adhesion may also involve lectin‐like components, present on organisms, which bind complementary carbohydrates tooth. Therefore, work aimed evaluate potential six lectins, extracted from seeds Leguminosae family members, inhibit adherence five species pellicle in vitro. Methods and Results: The lectins used were Canavalia...
The initial colonization of the tooth by streptococci involves their attachment to adsorbed components acquired pellicle. Avoiding this adhesion may be successful in preventing caries at early stages. Salivary mucins are glycoproteins that when absorbed onto hydroxyapatite provide binding sites for certain bacteria. Algal lectins especially interesting oral antiadhesion trials because great stability and high specificity mucins. This work aimed evaluate potential two algal inhibit adherence...
Light absorption of $\ensuremath{\alpha}$-glycine crystals grown by slow evaporation at room temperature was measured, indicating a $5.11\ifmmode\pm\else\textpm\fi{}0.02\phantom{\rule{0.3em}{0ex}}\mathrm{eV}$ energy band gap. Structural, electronic, and optical properties were obtained first-principles quantum mechanical calculations using density functional theory within the generalized gradient approximation in order to understand this result. To take into account contribution core...
When activated by amino acid starvation, the stress sensing protein kinase GCN2 phosphorylates eukaryotic initiation factor 2 alpha, inhibiting translation to conserve energy and facilitate cell survival. Amino particularly of tryptophan arginine, affects immune tolerance suppressing differentiation proliferation T-cells via activation kinase. In addition, pathway mediates cancer survival directly within context metabolic stress. Here, we report first crystal structures human domain (KD) in...
The carbohydrate-binding activity of the algal lectins from closely related red marine species Bryothamnion triquetrum (BTL) and seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect their membrane glyco-receptors. These interacted cells tested in a dose-dependent manner. Moreover, fluorescence spectra both clearly differentiated as shown by FACS profiles. Furthermore, observed confocal microscopy, BTL BSL bound surface glycoproteins underwent intense...