A5001881614- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- IL-33, ST2, and ILC Pathways
- Psoriasis: Treatment and Pathogenesis
- Viral-associated cancers and disorders
- Calcium signaling and nucleotide metabolism
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Asthma and respiratory diseases
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Advanced Breast Cancer Therapies
- Dermatology and Skin Diseases
- Endometriosis Research and Treatment
- NF-κB Signaling Pathways
- Multiple Myeloma Research and Treatments
- Retinoids in leukemia and cellular processes
- CAR-T cell therapy research
- Vibrio bacteria research studies
- Chronic Myeloid Leukemia Treatments
- Allergic Rhinitis and Sensitization
- Nuclear Structure and Function
University Children's Hospital Zurich
2014-2022
University of Zurich
2009-2021
Oslo University Hospital
2013-2017
University of Oslo
2013-2017
ETH Zurich
2006
There is increasing interest in the chronic lymphocytic leukemia (CLL) microenvironment and mechanisms that may promote CLL cell survival proliferation. A role for T helper (Th) cells has been suggested, but current evidence only circumstantial. Here we show patients had memory Th were specific endogenous antigens. These activated autologous proliferation vitro human → mouse xenograft experiments. Moreover, efficient antigen-presenting could endocytose process complex proteins through...
Abstract Forkhead box p3 (FOXP3) is known to program the acquisition of suppressive capacities in CD4+ regulatory T cells (Treg), whereas its role CD8+ unknown. The current study investigates whether FOXP3 also acts as a Treg master switch peripheral blood and tonsillar cells. Single-cell analyses reveal existence FOXP3+CD8+ population human tonsils, are rarely detected blood. Tonsillar exhibit phenotype with high CTLA-4 CD45RO low CD127 CD69 expression. Interestingly, mostly CD25negative...
Abstract Impaired functional activity of T regulatory cells has been reported in allergic patients and results an increased susceptibility to autoimmune diseases. The master regulator cell differentiation, the transcription factor FOXP3, is required for both their development function. Despite its key role, relatively little known about molecular mechanisms regulating foxp3 gene expression. In present study, effect Th1 cytokines on human differentiation was analyzed at epigenetic expression...
The SUN proteins are a conserved family of in eukaryotes. Human UNC84A (Sun1) is homolog Caenorhabditis elegans UNC‐84, protein involved nuclear anchorage and migration. We have analyzed targeting to the envelope (NE) show that N‐terminal 300 amino acids crucial for efficient NE localization whereas C‐terminal domain not required. Furthermore, we demonstrate by combining RNA interference with immunofluorescence fluorescence recovery after photobleaching analysis anchoring dependent on lamin...
Abstract Chronic lymphocytic leukemia (CLL) is a B cell malignancy associated with increased levels of inflammatory cytokines. Similarly, expression CD38 on CLL cells correlates survival and proliferation, but the mechanisms that regulate cytokines remain unclear. We have recently demonstrated patients CLL-specific Th support proliferation. In this article, we show attract such cells, thereby establishing an Ag-dependent collaboration. Blocking experiments performed in vitro as wells vivo,...
Abstract The process of Th cell differentiation toward polarized effector T cells tailors specific immunity against invading pathogens while allowing tolerance commensal microorganisms, harmless allergens, or autologous Ags. Identification the mechanisms underlying this polarization is therefore central to understand how immune system confers and tolerance. present study demonstrates that retinoic acid receptor-related orphan receptor C2 (RORC2), a key transcription factor in Th17...
Background Chronic rhinosinusitis (CRS) is characterized by epithelial activation and chronic T-cell infiltration in sinonasal mucosa nasal polyps. IL-33 a new cytokine of the IL-1 family that has pro-inflammatory Th2 type induction property. The role pathomechanisms CRS its interaction with other T cell subsets remain to be fully understood. Methods main trigger for mRNA expression primary human cells was determined multiple stimulated cultures. effects on naïve, Th0 memory T-cells studied...
Abstract Around 30–50% of classical Hodgkin lymphoma (cHL) cases in immunocompetent individuals from industrialized countries are associated with the B-lymphotropic Epstein-Barr virus (EBV). Although natural killer (NK) cells exhibit anti-viral and anti-tumoral functions, virtually nothing is known about quantitative qualitative differences NK patients EBV+ cHL vs. EBV- cHL. Here, we prospectively investigated 36 without immune suppression or overt immunodeficiency at diagnosis. All 10 25...
Endemic Burkitt lymphoma (eBL) is characterized by an oncogenic IGH/c-MYC translocation and Epstein-Barr virus (EBV) positivity, epidemiologically linked to Plasmodium falciparum malaria. Both EBV malaria are thought contribute eBL inducing the expression of activation-induced cytidine deaminase (AID), enzyme involved in translocation. AID/apolipoprotein B mRNA editing catalytic polypeptide-like (AID/APOBEC) family enzymes have recently emerged as potent mutagenic sources a variety cancers,...
Abstract Endemic Burkitt lymphoma (eBL) is an aggressive B cell cancer characterized by IgH/c-myc translocation and the harboring of Epstein–Barr virus (EBV). Evidence accumulates that CD4 + T cells might contribute to eBL pathogenesis. Here, we investigate presence in primary tissue their potential dichotomous impact on EBV-infected pre-eBL model using ex vivo material vitro co-cultures. In addition, establish a novel method study effect employing CRISPR/Cas9 knock-in approach introduce tag...
Abstract B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common childhood malignancy. The two-step BCP-ALL pathogenesis requires in utero-induced chromosomal aberrations and additional mutagenic events for overt leukemia. In mouse models, activation-induced cytidine deaminase (AID/ AICDA ) was suggested to contribute by off-target activity. role of AID patients, however, remains unclear. Moreover, usually not expressed B-cells but germinal center B-cells, where it induced...
Malaria is still a major health problem in sub-Saharan Africa and south-east Asia, but research on malaria low-income countries can be challenge due to the lack of laboratory equipment. In addition, severe mainly affects very young children, which limits amount blood available for purposes. Thus, there need protocols that yield maximum information from minimum blood, are operable basically equipped laboratories. A protocol tandem B T helper (Th) cell isolation directly whole...