A5083960916- Osteoarthritis Treatment and Mechanisms
- DNA Repair Mechanisms
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Knee injuries and reconstruction techniques
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Tracheal and airway disorders
- RNA Research and Splicing
- Cardiac, Anesthesia and Surgical Outcomes
- Anesthesia and Sedative Agents
- Mesenchymal stem cell research
- Telomeres, Telomerase, and Senescence
- Airway Management and Intubation Techniques
- Musculoskeletal pain and rehabilitation
- Voice and Speech Disorders
- Lower Extremity Biomechanics and Pathologies
- Foot and Ankle Surgery
- Mechanical Circulatory Support Devices
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cytokine Signaling Pathways and Interactions
- Connective tissue disorders research
- Galectins and Cancer Biology
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2024
Istituto Ortopedico Rizzoli
2014-2024
University of Padua
2023-2024
Policlinico S.Orsola-Malpighi
2024
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2016-2023
Orthopedic Institute
2014
Azienda Sanitaria Ospedaliera S.Croce e Carle Cuneo
2004
University of Bologna
1995-2002
Hahnemann University Hospital
1995
ABSTRACT Cytotoxicity assays provide an in vitro evaluation of the lytic activity NK and T cells against tumors or transformed cells. However, none these methods allow recovery supernatants after assay. We standardized a microcytotoxicity test using calcein-acetoxymethyl (calcein-AM) dye that requires very small quantities while maintaining same sensitivity as traditional 51 Cr The assay is applicable to resting well activated human effector uses different targets such cell lines are...
In this study we investigated whether purified NK cells, derived from a group of nonagenarian healthy subjects, were able to produce the chemokines MIP-1alpha, RANTES and IL-8, also characterized effect IL-12 or IL-2 immunomodulatory cytokines (that are among most effective inducers lytic activity soluble factor secretion) on induction, in vitro, these modulation corresponding receptors. This provides evidence that human cells subjects over 90 years old retain ability synthesize Rantes IL-8...
Adolescent idiopathic scoliosis (AIS) is a progressive deformity of the spine. Scoliotic curves progress until skeletal maturity leading, in rare cases, to severe deformity. While Cobb angle straightforward tool initial curve magnitude measurement, assessing risk progression at time diagnosis may be more challenging. Epigenetic and genetic markers are potential prognostic tools predict progression. The aim this study review available literature regarding epigenetic factors associated with...
To link matrix metalloproteinase 13 (MMP-13) activity and extracellular (ECM) remodeling to alterations in regulatory factors leading a disruption chondrocyte homeostasis.MMP-13 expression was ablated primary human chondrocytes by stable retrotransduction of short hairpin RNA. The effects MMP-13 knockdown on key regulators differentiation (SOX9, runt-related transcription factor 2 [RUNX-2], beta-catenin) angiogenesis (vascular endothelial growth [VEGF]) were scored at the protein level (by...
Mesenchymal stromal cells are emerging as an extremely promising therapeutic agent for tissue regeneration due to their multi-potency, immune-modulation and secretome activities, but safety remains one of the main concerns, particularly when in vitro manipulation, such cell expansion, is performed before clinical application. Indeed, it well documented that expansion reduces replicative potential some multi-potency promotes senescence. Furthermore, during aging there a decrease DNA synthesis...
Mechanical stimulation appears to play a key role in cartilage homeostasis maintenance, but it can also contribute osteoarthritis (OA) pathogenesis. Accumulating evidence suggests that loading the physiological range contributes tissue integrity whereas excessive or reduced have catabolic effects. However, how mechanical stimuli regulate joint is still not completely elucidated and few data are available on human cartilage. We aimed at investigating OA response ex vivo intensity. Cartilage...
In muscle cells subjected to mechanical stimulation, LINC complex and cytoskeletal proteins are basic preserve cellular architecture maintain nuclei orientation positioning. this context, the role of lamin A/C remains mostly elusive. This study demonstrates that in human myoblasts stretching, recruits desmin plectin nuclear periphery, allowing a proper spatial nuclei. Interestingly, Emery-Dreifuss Muscular Dystrophy (EDMD2) exposed recruitment nucleus were impaired, suggesting functional is...
The Proviral Integration site for the Moloney murine leukemia virus (PIM)-1 kinase and its family members (PIM-2 PIM-3) regulate several cellular functions including survival, proliferation, apoptosis. Recent studies showed their involvement in pathogenesis of rheumatoid arthritis RA, while no are available on psoriatic (PsA) axial spondyloarthritis (axSpA). main objective this study is to assess expression PIM kinases inflammatory arthritides, correlation with proinflammatory cytokines,...
Age-related alterations of DNA repair could be involved in the accumulation genetic damage with age. Few data suggest a possible alteration age mismatch system, evidenced by acquisition microsatellite instability. We aimed to point out implication this system Peripheral blood cell from 226 participants, 110 young (25–35 years), 58 old (85–97 and centenarian was analyzed at five polymorphic loci (CD4, p53, VWA31, TPOX, FES/FPS) age-related instabilities or modifications allele frequencies....
Different sources of mesenchymal stromal cells can be considered for regenerative medicine applications. Here we analyzed human adipose-derived from infrapatellar fat pad (IFPSC) osteoarthritis patients, representing a very interesting candidate cartilage regeneration. No data are available concerning IFPSC stability after in vitro expansion. Indeed, replicative potential and multipotency progressively decrease during culture passages while DNA damage cell senescence increase, thus possibly...
SUMMARY We investigated the effect of proinflammatory cytokine interleukin 17 (IL-17) on lysis osteosarcoma cells by human NK cells. and U-2 OS, MG-63, HOS cell lines express IL-17 receptor, highest amount being found OS. Pre-incubation with did not affect cytotoxicity against osteosarcomas, that was increased when OS were pre-incubated IL-17. In treated FACS analysis demonstrated an expression fibronectin among panel adhesion molecules assayed, treatment anti-fibronectin antibodies...
The cell membrane is a primary and fundamental player in most cellular processes, fatty acids form major structural component of membranes. aim this study was to compare the acid profiles different human blood leukocytes selected lines, identify effects vitro culture on profiles, test medium supplements for their effect profiles. Different classes were isolated from analysed compared. After culturing immortalised leukocytes, Finally, lipid formulations developed used supplementing an effort...
Introduction Obesity can worsen fibromyalgia (FM) and very low-calorie ketogenic diet (VLCKD) is a potential therapeutic option for diseases that share clinical pathophysiological features with FM. In this pilot interventional study, we investigated the effects of VLCKD in obese women Methods Female patients FM body mass index (BMI) ≥ 30 kg/m 2 were eligible VLCKD. The phase (T0 to T8) was followed by progressive reintroduction carbohydrates (T8 T20). Changes BMI, Fibromyalgia Impact...