A5080595673- Viral Infections and Immunology Research
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Insect Resistance and Genetics
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Caveolin-1 and cellular processes
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Glycosylation and Glycoproteins Research
- Mosquito-borne diseases and control
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Signaling Pathways in Disease
National Institute of Allergy and Infectious Diseases
2020
National Institutes of Health
2020
University of California, Irvine
2015-2018
SomaLogic (United States)
2010
Background The interrogation of proteomes ("proteomics") in a highly multiplexed and efficient manner remains coveted challenging goal biology medicine. Methodology/Principal Findings We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 µL serum or plasma). Our current assay measures 813 with low limits detection (1 pM median), 7 logs overall dynamic range (∼100 fM–1 µM), 5% median...
Abstract Interrogation of the human proteome in a highly multiplexed and efficient manner remains coveted challenging goal biology. We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 [mu]L serum or plasma). Our current assay allows us to measure ~800 with very low limits detection (1 pM average), 7 logs overall dynamic range, 5% average coefficient variation. This is enabled by generation...
Repurposing FDA-approved drugs that treat respiratory infections caused by coronaviruses, such as SARS-CoV-2 and MERS-CoV, could quickly provide much needed antiviral therapies. In the current study, potency cellular toxicity of four fluoroquinolones (enoxacin, ciprofloxacin, levofloxacin, moxifloxacin) were assessed in Vero cells A549 engineered to overexpress ACE2, entry receptor. All suppressed replication at high micromolar concentrations both cell types, with enoxacin demonstrating...
Protein production, genomic RNA replication, and virion assembly during infection by picornaviruses like human rhinovirus poliovirus take place in the cytoplasm of infected cells, making them quintessential cytoplasmic pathogens. However, a growing body evidence suggests that picornavirus replication is promoted number host proteins localized normally within cell nucleus. To systematically identify such nuclear proteins, we focused on those appear to re-equilibrate from nucleus HeLa cells...
Despite being intensely studied for more than 50 years, a complete understanding of the enterovirus replication cycle remains elusive. Specifically, only handful cellular proteins have been shown to be involved in RNA these viruses. In an effort isolate and identify additional that function enteroviral replication, we generated multiple recombinant polioviruses containing affinity tags within 3' or 5' noncoding region genome. These viruses retained sequences genome while remaining viable...
Caveolin-1 is a target for academic and pharmaceutical research due to its many cellular roles associated diseases. We report peptide WL47 (1), small, high-affinity, selective disrupter of caveolin-1 oligomers. Developed optimized through screening analysis synthetic libraries, ligand 1 has 7500-fold improved affinity compared T20 parent an 80% decrease in sequence length. Ligand will permit targeted study function.
Powassan virus (POWV) is the only tick-borne flavivirus (TBFV) known to circulate in North America. Although there are relatively few documented cases of POWV disease, investigations into justified due increasing incidence infection and significant case fatality rate associated with this virus. To better describe molecular biology replication cycle mammalian cells, we performed genome-scale screens uncover host factors required for viral replication. Putative proviral were identified by...