A5041877631- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Radiomics and Machine Learning in Medical Imaging
- Bacterial Genetics and Biotechnology
- Cardiovascular Health and Risk Factors
- Liver Disease Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Bioinformatics and Genomic Networks
- Health, Environment, Cognitive Aging
- Machine Learning in Bioinformatics
- Diabetes Treatment and Management
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Occupational and environmental lung diseases
- Gene expression and cancer classification
- Monoclonal and Polyclonal Antibodies Research
- Liver Diseases and Immunity
- Endoplasmic Reticulum Stress and Disease
- Nutrition, Genetics, and Disease
- Medical Imaging and Pathology Studies
- Cancer, Lipids, and Metabolism
- Heart Failure Treatment and Management
- RNA and protein synthesis mechanisms
SomaLogic (United States)
2015-2025
University of Basel
2023
University of Westminster
2023
Prevention of Organ Failure
2021
University of California, San Francisco
2017-2018
National Research Council
2018
University of Colorado Denver
2014
Heidelberg University
2014
University Hospital Heidelberg
2014
University of Colorado Cancer Center
2014
Background The interrogation of proteomes ("proteomics") in a highly multiplexed and efficient manner remains coveted challenging goal biology medicine. Methodology/Principal Findings We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 µL serum or plasma). Our current assay measures 813 with low limits detection (1 pM median), 7 logs overall dynamic range (∼100 fM–1 µM), 5% median...
Abstract Interrogation of the human proteome in a highly multiplexed and efficient manner remains coveted challenging goal biology. We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 [mu]L serum or plasma). Our current assay allows us to measure ~800 with very low limits detection (1 pM average), 7 logs overall dynamic range, 5% average coefficient variation. This is enabled by generation...
Lung cancer is the leading cause of deaths worldwide. New diagnostics are needed to detect early stage lung because it may be cured with surgery. However, most cases diagnosed too late for curative Here we present a comprehensive clinical biomarker study and first large-scale application new aptamer-based proteomic technology discover blood protein biomarkers in disease.We conducted multi-center case-control archived serum samples from 1,326 subjects four independent studies non-small cell...
Pseudomonas aeruginosa produces two secreted phospholipase C (PLC) enzymes. The expression of both PLCs is regulated by Pi. One the hemolytic, and one nonhemolytic. Low-stringency hybridization studies suggested that genes encoding these shared DNA homology. This information was used to clone plcN, gene 77-kilodalton nonhemolytic PLC, PLC-N. A fragment plcN mutate chromosomal copy generation a interruption mutation. mutant 55% less total PLC activity than wild type, confirming successful...
Background Malignant pleural mesothelioma (MM) is an aggressive, asbestos-related pulmonary cancer that increasing in incidence. Because diagnosis difficult and the disease relatively rare, most patients present at a clinically advanced stage where possibility of cure minimal. To improve surveillance detection MM high-risk population, we completed series clinical studies to develop noninvasive test for early detection. Methodology/Principal Findings We conducted multi-center case-control...
Abstract Understanding the genetic architecture of host proteins interacting with SARS-CoV-2 or mediating maladaptive response to COVID-19 can help identify new repurpose existing drugs targeting those proteins. We present a discovery study 179 such among 10,708 individuals using an aptamer-based technique. 220 DNA sequence variants acting in cis (MAF 0.01-49.9%) and explaining 0.3-70.9% variance 97 these proteins, including 45 no previously known protein quantitative trait loci (pQTL) 38...
A reliable, individualized, and dynamic surrogate of cardiovascular risk, synoptic for key biologic mechanisms, could shorten the path drug development, enhance cost-effectiveness improve patient outcomes. We used highly multiplexed proteomics to address these objectives, measuring about 5000 proteins in each 32,130 archived plasma samples from 22,849 participants nine clinical studies. machine learning derive a 27-protein model predicting 4-year likelihood myocardial infarction, stroke,...
Abstract Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple proteins interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, can be vigorously washed remove background proteins, providing potential superior signal‐to‐noise ratios and lower limits quantification biological matrices. Data presented here a 17‐plex photoaptamer array exhibiting detection below 10 fM...
The phospholipase C (PLC) operon of Pseudomonas aeruginosa consists plcS, which encodes a heat-labile secreted hemolysin, and two in-phase, overlapping genes, plcR1 plcR2, may encode Pi-regulatory genes. A 2.8-kilobase-pair deletion mutation in this was constructed, tetracycline resistance (Tcr) cartridge replaced the deleted sequences. mutant strain PAO1 obtained through recombination between flanking regions mutated cloned PLC homologous chromosomal regions. its replacement by Tcr...
Lung cancer remains the most common cause of cancer-related mortality. We applied a highly multiplexed proteomic technology (SOMAscan) to compare protein expression signatures non small-cell lung (NSCLC) tissues with healthy adjacent and distant from surgical resections. In this first report SOMAscan tissues, we highlight 36 proteins that exhibit largest differences between matched tumor non-tumor tissues. The concentrations twenty increased sixteen decreased in tissue, thirteen which are...
ABSTRACT New non-sputum biomarker tests for active tuberculosis (TB) diagnostics are of the highest priority global TB control. We performed in-depth proteomic analysis using 4,000-plex SOMAscan assay on 1,470 serum samples from seven countries where is endemic. All were patients with symptoms and signs suggestive pulmonary that systematically confirmed or ruled out by culture clinical follow-up. HIV coinfection was present in 34% samples, 25% sputum smear negative. Serum protein biomarkers...
CT screening for lung cancer is effective in reducing mortality, but there are areas of concern, including a positive predictive value 4% and development interval cancers. A blood test that could manage these limitations would be useful, such tests has been impaired by variations collection may lead to poor reproducibility across populations.Blood-based proteomic profiles were generated with SOMAscan technology, which measured 1033 proteins. First, preanalytic variability was evaluated...
OBJECTIVE Coronary artery disease (CAD) is a major challenge in patients with type 2 diabetes (T2D). computed tomography angiography (CCTA) provides detailed anatomic map of the coronary circulation. Proteomics are increasingly used to improve diagnostic and therapeutic algorithms. We hypothesized that protein panel differentially associated T2D CAD. RESEARCH DESIGN AND METHODS In CAPIRE (Coronary Atherosclerosis Outlier Subjects: Protective Novel Individual Risk Factors Evaluation—a cohort...
HFrEF is a heterogenous condition with high mortality. We used serial assessments of 4210 circulating proteins to identify distinct novel protein-based subphenotypes and investigate underlying dynamic biological mechanisms. Herewith we aimed gain pathophysiological insights fuel opportunities for personalised treatment.In 382 patients, performed trimonthly blood sampling during median follow-up 2.1 [IQR:1.1-2.6] years. selected all baseline samples two closest the primary endpoint (PEP;...
Early detection of adverse effects novel therapies and understanding their mechanisms could improve the safety efficiency drug development. We have retrospectively applied large-scale proteomics to blood samples from ILLUMINATE (Investigation Lipid Level Management Understand its Impact in Atherosclerotic Events), a trial torcetrapib (a cholesterol ester transfer protein inhibitor), that involved 15 067 participants at high cardiovascular risk. was terminated median 550 days because...
OBJECTIVE To assess the effects of empagliflozin, a selective sodium–glucose cotransporter 2 (SGLT2) inhibitor, on broad biological systems through proteomics. RESEARCH DESIGN AND METHODS Aptamer-based proteomics was used to quantify 3,713 proteins in 144 paired plasma samples obtained from 72 participants across spectrum glucose tolerance before and after 4 weeks empagliflozin 25 mg/day. The biology significantly changed by (at false discovery rate–corrected P < 0.05) discerned...
Detecting NASH remains challenging, while at-risk (steatohepatitis and F≥ 2) tends to progress is of interest for drug development clinical application. We developed prediction models by supervised machine learning techniques, with data biomarkers stage grade patients NAFLD.Learning were collected in the Liver Investigation: Testing Marker Utility Steatohepatitis metacohort (966 biopsy-proven NAFLD adults), staged graded according CRN. Conditions trial definition (NAS ≥ 4;53%), (NASH F...