Alexandre F.R. Stewart
A5001575849- Genetic Associations and Epidemiology
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Cardiomyopathy and Myosin Studies
- Congenital heart defects research
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- BRCA gene mutations in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Cardiovascular Function and Risk Factors
- Music History and Culture
- Advanced biosensing and bioanalysis techniques
- Genomics and Rare Diseases
- Cardiovascular Effects of Exercise
- Protein Tyrosine Phosphatases
- Cardiac electrophysiology and arrhythmias
- Muscle Physiology and Disorders
- Advanced Causal Inference Techniques
- RNA and protein synthesis mechanisms
University of Ottawa
2016-2025
Institute of Infection and Immunity
2020-2025
Canadian Heart Research Centre
2010-2024
Ottawa University
2024
Great Ormond Street Hospital for Children NHS Foundation Trust
2023
University College London
2023
Centre Alexandre Koyré
2011-2020
WinnMed
2020
University of Leicester
2010-2017
University of Pennsylvania
2010-2017
Coronary heart disease (CHD) is a major cause of death in Western countries. We used genome-wide association scanning to identify 58-kilobase interval on chromosome 9p21 that was consistently associated with CHD six independent samples (more than 23,000 participants) from four Caucasian populations. This interval, which located near the CDKN2A and CDKN2B genes, contains no annotated genes not established risk factors such as plasma lipoproteins, hypertension, or diabetes. Homozygotes for...
Background The interrogation of proteomes ("proteomics") in a highly multiplexed and efficient manner remains coveted challenging goal biology medicine. Methodology/Principal Findings We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 µL serum or plasma). Our current assay measures 813 with low limits detection (1 pM median), 7 logs overall dynamic range (∼100 fM–1 µM), 5% median...
Making sense of rapidly evolving evidence on genetic associations is crucial to making genuine advances in human genomics and the eventual integration this information into practice medicine public health. Assessment strengths weaknesses evidence, hence ability synthesize it, has been limited by inadequate reporting results. The STrengthening REporting Genetic Association studies (STREGA) initiative builds Reporting Observational Studies Epidemiology (STROBE) Statement provides additions 12...
Atrial fibrillation is the most common type of cardiac arrhythmia and a leading cause cardiovascular morbidity, particularly stroke. The gap-junction protein connexin 40 expressed selectively in atrial myocytes mediates coordinated electrical activation atria. We hypothesized that idiopathic has genetic basis tissue-specific mutations GJA5, gene encoding 40, may predispose atria to fibrillation.We sequenced GJA5 from genomic DNA isolated resected tissue peripheral lymphocytes 15 patients...
Coronary heart disease (CHD), the commonest cause of death worldwide, is highly heritable, but DNA sequence variations associated with elevated cardiovascular risk are largely unknown. The investigators planned a genome-wide associational study based on 100,000 single nucleotide polymorphisms and involving 3 sequential case-control comparisons made at nominal significance threshold P < 0.025. population included more than 23,000 participants from 4 Caucasian populations. Cases had severe,...
Genetic studies might provide new insights into the biological mechanisms underlying lipid metabolism and risk of CAD. We therefore conducted a genome-wide association study to identify novel genetic determinants low-density lipoprotein cholesterol (LDL-C), high-density (HDL-C), triglycerides.We combined data from 8 studies, comprising up 17 723 participants with information on circulating concentrations. did independent replication in 37 774 populations also population Indian Asian descent....
We have investigated the functional significance of conserved sequences within 9p21.3 risk locus for coronary artery disease (CAD) and determined relationship to expression ANRIL whole genome gene expression.We demonstrate that a sequence has enhancer activity variant significantly increases reporter in primary aortic smooth muscle cells. Whole blood RNA short variants was increased by 2.2-fold whereas long decreased 1.2-fold healthy subjects homozygous allele. Expression levels were...
Abstract Interrogation of the human proteome in a highly multiplexed and efficient manner remains coveted challenging goal biology. We present new aptamer-based proteomic technology for biomarker discovery capable simultaneously measuring thousands proteins from small sample volumes (15 [mu]L serum or plasma). Our current assay allows us to measure ~800 with very low limits detection (1 pM average), 7 logs overall dynamic range, 5% average coefficient variation. This is enabled by generation...
A high-resolution analysis of copy number variation in patients with ADHD reveals new gene associations, few de novo mutations, and overlap genes implicated other disorders such as autism.
Abstract Making sense of rapidly evolving evidence on genetic associations is crucial to making genuine advances in human genomics and the eventual integration this information practice medicine public health. Assessment strengths weaknesses evidence, hence ability synthesize it, has been limited by inadequate reporting results. The STrengthening REporting Genetic Association studies (STREGA) initiative builds Reporting OBservational Studies Epidemiology (STROBE) Statement provides additions...
Mackerels, tunas, and billfishes (suborder Scombroidei Teleostei) provide an ideal taxonomic context in which to examine the evolution of endothermy. Multiple origins diverse strategies for endothermy exist among these fish. Here a molecular phylogeny has been determined by direct sequencing portion mitochondrial cytochrome b gene. The distribution endothermic species within this proposed genealogy indicates that ability warm brain retina arose independently three lineages, each time...
Making sense of rapidly evolving evidence on genetic associations is crucial to making genuine advances in human genomics and the eventual integration this information practice medicine public health. Assessment strengths weaknesses evidence, hence ability synthesize it, has been limited by inadequate reporting results. The STrengthening REporting Genetic Association studies (STREGA) initiative builds Reporting OBservational Studies Epidemiology (STROBE) Statement provides additions 12 22...
Lung cancer is the leading cause of deaths worldwide. New diagnostics are needed to detect early stage lung because it may be cured with surgery. However, most cases diagnosed too late for curative Here we present a comprehensive clinical biomarker study and first large-scale application new aptamer-based proteomic technology discover blood protein biomarkers in disease.We conducted multi-center case-control archived serum samples from 1,326 subjects four independent studies non-small cell...