A5067726123- Muscle Physiology and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Neurogenetic and Muscular Disorders Research
- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Nerve injury and regeneration
- Zebrafish Biomedical Research Applications
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Metabolism, Diabetes, and Cancer
- Hematopoietic Stem Cell Transplantation
- Axon Guidance and Neuronal Signaling
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Diabetes and associated disorders
- GDF15 and Related Biomarkers
- Single-cell and spatial transcriptomics
- Adipokines, Inflammation, and Metabolic Diseases
- Extracellular vesicles in disease
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Signaling Pathways in Disease
- Neuroscience and Neural Engineering
- Hypothalamic control of reproductive hormones
Stanford University
2020-2024
Palo Alto University
2024
University of Michigan
2018-2024
Neurosciences Institute
2021
Joslin Diabetes Center
2008-2015
Harvard University
2008-2015
Harvard Stem Cell Institute
2008-2015
Howard Hughes Medical Institute
2010-2015
Brigham and Women's Hospital
2013
Boston University
2006
Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting blood contains humoral "rejuvenating" factors can restore regenerative function. Here, we demonstrate the circulating protein growth differentiation factor 11 (GDF11) rejuvenating for skeletal muscle. Supplementation of systemic GDF11 levels, which normally decline with age, heterochronic parabiosis or delivery recombinant protein, reversed functional...
Brown fat is specialized for energy expenditure and has therefore been proposed to function as a defense against obesity. Despite recent advances in delineating the transcriptional regulation of brown adipocyte differentiation, cellular lineage specification developmental cues specifying brown-fat cell fate remain poorly understood. In this study, we identify isolate subpopulation adipogenic progenitors (Sca-1 + /CD45 − /Mac1 ; referred Sca-1 progenitor cells, ScaPCs) residing murine fat,...
Integrative organ crosstalk regulates key aspects of energy homeostasis, and its dysregulation may underlie metabolic disorders such as obesity diabetes. To test the hypothesis that between liver pancreatic islets modulates β cell growth in response to insulin resistance, we used liver-specific receptor knockout (LIRKO) mouse, a unique model exhibits dramatic islet hyperplasia. Using complementary vivo parabiosis transplantation assays, well vitro culture approaches, demonstrate humoral,...
Axotomized spinal motoneurons (MNs) lose presynaptic inputs following peripheral nerve injury; however, the cellular mechanisms that lead to this form of synapse loss are currently unknown. Here, we delineate a critical role for neuronal kinase dual leucine zipper (DLK)/MAP3K12, which becomes activated in axotomized neurons. Studies with conditional knockout mice indicate DLK signaling activation injured MNs triggers induction phagocytic microglia and loss. Aspects DLK-regulated response...
Pax7 is a key regulator of skeletal muscle stem cells and required along with Pax3 to generate precursors. We have identified collection genes induced by either or in C2C12 cells. Two notable Pax3/7 targets are the inhibitory helix-loop-helix (HLH) proteins inhibitor DNA binding (Id) 2 Id3, both which coordinately expressed quiescent satellite myogenic after ectopic expression Pax7. Ectopic activates luciferase reporter driven Id3 promoter, maximal induction this requires conserved site...
Abstract The thymus reaches its maximum size early in life and then begins to shrink, producing fewer T cells with increasing age. This thymic decline is thought contribute age-related cell lymphopenias hinder recovery after bone marrow transplantation. Although several cellular molecular processes have been implicated involution, their relative contributions are not known. Using heterochronic parabiosis, we observe that young circulating factors sufficient drive regeneration of the aged...
Abstract The neuromuscular junction (NMJ) is a specialized synapse that formed by motor axon innervation of skeletal muscle fibers. maintenance motor-muscle connectivity critical for the preservation tone and generation movement. Injury can induce robust regenerative response in axons, but severe trauma or chronic denervation resulting from neurodegenerative disease typically leads to inefficient repair poor functional recovery. guidance molecule Semaphorin3A (Sema3A) has been implicated as...
Abstract The spinal cord is a fascinating structure responsible for coordinating all movement in vertebrates. Spinal motor neurons control the activity of virtually every organ and muscle throughout body by transmitting signals that originate cord. These are remarkably heterogeneous their innervation targets. However, because represent only small fraction cells within difficult to isolate, full complement neuron subtypes remains unknown. Here we comprehensively describe molecular...
The ventromedial hypothalamus (VMH) is an oestrogen‐responsive area known to facilitate female sexual behaviour in the rat. VMH innervated by noradrenergic neurones projecting from brain stem, and it has been demonstrated that noradrenaline receptor activation plays a role expression of lordosis reflex. Noradrenaline shown be released within after receives vaginocervical stimulation (VCS) male during mating. VCS also required induce twice‐daily surges prolactin (PRL) characteristic early...
Abstract Understanding spinal cord generation and assembly is essential to elucidate how motor behavior controlled disorders arise. The cellular landscape of the human remains, however, insufficiently explored. Here, we profiled midgestation with single cell-resolution discovered, even at this fetal stage, remarkable heterogeneity across within cell types. Glia displayed diversity related positional identity along dorso-ventral rostro-caudal axes, while astrocytes specialized transcriptional...
ABSTRACT Astrocytes are emerging as key regulators of neuronal synaptic network maturation and function, through control pruning. This is important, because individuals with ASD have excess glutamatergic synapses in the cortex, but biological mechanisms underlying this phenotype remain unclear. Here, we used human cortical organoids (hCO) derived from induced pluripotent stem cells (hiPSCs), to examine effect hypoxia on synapse engulfment astrocytes at postnatal-equivalent stages...
(Cell Reports 3, 401–410; January 31, 2013) In the original version of this article, affiliation authors Jennifer L. Shadrach and Amy J. Wagers was written incorrectly as Howard Hughes Medical institute, Chevy Chase, MD 20815-6789, USA. The correct is Institute, Harvard University, Cambridge, MA 02138, This has since been corrected online, appears here well. regret error. Liver-Derived Systemic Factors Drive β Cell Hyperplasia in Insulin-Resistant StatesEl Ouaamari et al.Cell ReportsJanuary...